LOCUS       sequence-258159-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector encoding the receptor MOR170-1 and the 
            barcoded CRE reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung 
            R, de March C, Bloom JS, Matsunami H, Kosuri S
  TITLE     A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions 
            with RNA Sequencing.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):254-260.e6. doi: 
            10.1016/j.cels.2019.02.009. Epub 2019 Mar 20.
  PUBMED    30904378
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.009"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "254-260.e6"
COMMENT     Sequence Label: pB-MultiplexedReporter-MOR170-1
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    106..124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    1690..1811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2057..2268
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2281..2549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     complement(3093..3113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    3155..3276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4039..4073
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4564..4580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4564..4580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4577..4599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4588..4604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4612..4618,4619..4636,4637..4642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4657..4678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4795..4812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4966..5554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5046..5065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5725..6516,6517..6585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6348..6367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6586..6690)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6716..7171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6853..6874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7065..7084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7298..7320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7312..7329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7313..7329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   7459..7521
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        8267..8298
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    9489..9623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gtaccagacc catcaagtgc cacctgacgt ctccctatca gtgatagaga agtcgacacg
       61 tctcgagctc cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata
      121 gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc tagaacgtct
      181 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
      241 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
      301 tgatagagaa ggtaccccct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      361 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctgag
      421 ctcgtttagt gaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      481 tgctaacgca gtcagtgctt ctggccgcca ccatgagacc ccagatcctg ctgctcctgg
      541 ccctgctgac cctaggcctg gctcatatga atggcacaga aggccctaac ttctacgtgc
      601 ccttctccaa tgcgacgggt gtggtacgcg aattcacgcg tatgccactc aatgcacaaa
      661 agactatgga aaatgattct tctgtgtctg agtttattct tatgggactg acagaccaac
      721 ctgagctcca gctgccctta tttgttctgt tcttggtgaa ctacacagtc actgtgatgg
      781 gaaatttgag cttaatgaat ctcatttgcc taaactcaaa ccttcacacc cccatgtact
      841 ttttcatctt caacctgtcc ttcattgact tctgttattc aatggtcttt acccccaaaa
      901 tgctgatggg ctttgttgta gagaagaaca tcatctcctt cagaggatgc atgactcagc
      961 tgtttttctt cctatttttt gtgaactctg agagttatgt gctgacagca atggcctatg
     1021 atcgctatgt ggccatctgt cagccactgc tctacaaagc tgttatgtca cctggcatct
     1081 gctttctatt gatattttgt acttatttga tggggcttgt tagtgcctta ttccatacag
     1141 gttttatgat aaggctcaac ttttgtgatt ctaatgttat caaccactat atgtgtgata
     1201 tcttccctct ctttcggctc tcctgcagta gcacctatct cactgagctt gtgagctctg
     1261 ctgtggtggg tacagctatt atcttatgtt gcctcattat cttaatctct tatggtatga
     1321 tcctttacaa tatcattcat atgtcatcag gtaagggttg gtccaaagcc ttgggcactt
     1381 gtggttctca cattataact gttagcttgt tctatgtaac tgggatgctt gcttatgtca
     1441 agccatcatc tgctgagact gtgggacagg ggaaaatttt ctcagtattt tacacttttt
     1501 tggtgcccat gctgaatcct cttatttaca gcctcaggaa caaggatgtc aaacttgctg
     1561 tgaaaaaaac ctggaagaga ctcacatgtt aagcggccgc ttcgagcaga catgataaga
     1621 cctgcaggtg gaaagactcc tcagcagggc cgactcttgt ccatgagcgg ccgcttcgag
     1681 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1741 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1801 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     1861 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg
     1921 gctggcgtaa tagcgaagag gggatcccca ggaagctcct ctgtgtcctc ataaacccta
     1981 acctcctcta cttgagagga cattccaatc atagggcggc cgcgaaggat ctgcgatcgc
     2041 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2101 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2161 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2221 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     2281 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     2341 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     2401 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     2461 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     2521 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gcttattcgt
     2581 gccgtgttat atgaaaacct tcaacatctc tcagcaggat ctggagctgg tggaggtcgc
     2641 cactgagaag atcaccatgc tctatgagga caacaagcac catgtcgggg cggccatcag
     2701 gaccaagact ggggagatca tctctgctgt ccacattgaa gcctacattg gcagggtcac
     2761 tgtctgtgct gaagccattg ccattgggtc tgctgtgagc aacgggcaga aggactttga
     2821 caccattgtg gctgtcaggc acccctactc tgatgaggtg gacagatcca tcagggtggt
     2881 cagcccctgt ggcatgtgta gagagctgat ctctgactat gctcctgact gctttgtgct
     2941 cattgagatg aatggcaagc tggtcaaaac caccattgag gaactcatcc ccctcaagta
     3001 caccaggaac taagggcaca gcaatcaaaa gtagtcgaca atcaacctct ggattacaaa
     3061 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3121 gctgctttaa tgcctttgta tcatgcgtta actaaacttg tttattgcag cttataatgg
     3181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3241 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg
     3301 atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa gacatccctg
     3361 tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac
     3421 caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca
     3481 ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc
     3541 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3601 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     3661 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     3721 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga
     3781 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     3841 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     3901 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     3961 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     4021 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
     4081 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
     4141 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
     4201 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
     4261 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
     4321 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
     4381 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
     4441 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
     4501 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
     4561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     6721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7141 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     7201 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     7261 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     7321 acggccagtg agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact
     7381 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
     7441 aacacgcagc tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     7501 catgtgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     7561 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     7621 aatttattta tgtttattta tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa
     7681 gtaacaaaac ttttatgagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     7741 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     7801 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     7861 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     7921 ggggaaaagg cctccacggc cactagtatt atgcccagta catgacctta tgaaccgtcg
     7981 tgacgtcaga cagaccacgc gatcgctcga gtccgccggt caatccggtg acgtcacggg
     8041 cctcttcgct attacgccag ctggcgaaag ggggttgacg tcacattaaa tcggccaacg
     8101 cgcggggaga ggcggtgacg tcaacaggca tcgtggtgtc acgctcgtcg tgacgtcagt
     8161 cgctttaact ggccctggct ttggcagcct gtgctcagcc tgacgtcaga ggcctgacgt
     8221 cagagagcct gacgtcagag agcctgacgt cagagagcgg agactctaga gggtatataa
     8281 tggaagctcg aattccagct tggcattccg gtactgttgg taaaatggtg tccaagggcg
     8341 aagaggacaa catggccatc atcaaagaat tcatgcggtt caaggtgcac atggaaggca
     8401 gcgtgaacgg ccacgagttc gagattgagg gcgagggcga aggcagaccc tacgagggaa
     8461 cacagaccgc caagctgaaa gtgaccaagg gcggacccct gcccttcgcc tgggatatcc
     8521 tgagccccca gtttatgtac ggcagcaagg cctacgtgaa gcaccccgcc gacatccccg
     8581 actacctgaa gctgagcttc cccgagggct tcaagtggga gagagtgatg aacttcgagg
     8641 acggcggcgt cgtgaccgtg acccaggata gctctctgca ggacggcgag ttcatctaca
     8701 aagtgaagct gcggggcacc aacttcccca gcgacggacc cgtgatgcag aaaaagacca
     8761 tgggctggga ggccagcagc gagagaatgt accctgagga tggcgccctg aagggcgaga
     8821 tcaagcagcg gctgaaactg aaggatggcg gccactacga cgccgaagtg aaaaccacct
     8881 acaaggccaa gaaacccgtg cagctgcctg gcgcctacaa cgtgaacatc aagctggaca
     8941 tcaccagcca caacgaggac tacaccatcg tggaacagta cgagcgggcc gagggcagac
     9001 actctacagg cggaatggac gagctgtaca agtgaacgcg tgtgctgtgg gctcctcgac
     9061 gcatctgatt aaagggggta ccttatgttc acaactggcg tgaatgtaac tgtattcagc
     9121 gatgacccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     9181 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     9241 agaaccggtg cgatgacaca ctagtccaca caggcataga gtgtctgcta ttaataacta
     9301 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     9361 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     9421 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     9481 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9601 atgtatctta tcatgtctgg atccccagga agctcctctg tgtcctcata aaccctaacc
     9661 tctggatccg aggacattcc aatcataggg
//
