LOCUS       sequence-258160-        6671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 12_pAAV-ProC2-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        912..1108
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      959..1094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1115..1124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1121..2047
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2051..2053,2054..2056,2057..2770)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2051..2053,2054..2056,2057..2767)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2096..2117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2357..2376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2704..2725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2798..3386
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2851..2871)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3269..3280)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3418..3894
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3617..3636)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3934..4074
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3934..4063
                     /label=AAV2 ITR
     rep_origin      4149..4604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4236..4255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4446..4467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4621..4640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4740..4762
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4800..4818)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4886..4990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4991..5059,5060..5851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5209..5228)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6022..6610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6511..6530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc
      181 aaacgccctc ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc
      241 tccccgatcc ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc
      301 tcctaccctc ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg
      361 cccctgagac ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct
      421 gttgcttggc ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg
      481 ttatgtacac ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga
      541 cctgaccgtc ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata
      601 ttgaatagtc ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg
      661 ggcgtgctgc ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct
      721 cgccactcac ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt
      781 ggaatagatc ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt
      841 aacttcattc ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaagctcga
      901 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      961 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt
     1021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1081 cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct
     1141 gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt
     1201 acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg
     1261 tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct
     1321 tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg
     1381 cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat
     1441 gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac
     1501 ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag
     1561 gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc
     1621 catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa
     1681 cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg
     1741 gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc
     1801 catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt
     1861 cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct
     1921 gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt
     1981 gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg
     2041 cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2101 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2161 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2221 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2281 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2341 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2401 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2461 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2521 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2581 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2641 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2701 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2761 gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3181 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     3241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3301 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3361 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     3421 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3481 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3541 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3601 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3661 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     3721 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3781 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3841 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     3901 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3961 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4021 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4081 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4141 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4201 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4441 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     4501 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     4561 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4621 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4681 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4741 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4801 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6661 tgctcacatg t
//
