LOCUS       sequence-258162-        6471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 9_pAAV-ProC5-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        712..908
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      759..894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             921..1847
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1851..1853,1854..1856,1857..2570)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1851..1853,1854..1856,1857..2567)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1896..1917)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2157..2176)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2504..2525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2598..3186
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2651..2671)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3069..3080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3218..3694
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3417..3436)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3734..3874
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3734..3863
                     /label=AAV2 ITR
     rep_origin      3949..4404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4036..4055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4246..4267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4421..4440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4540..4562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4600..4618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4686..4790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4791..4859,4860..5651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5009..5028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5822..6410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6311..6330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggcata agggggaacc aagcttaaac agtcccatgg
      181 ccaagcttct tattcgtggc gcacaagctt ggcgatggcc aaagccaagc ttactgagtg
      241 caactgtcaa gcttcactat cagcgatttc aagcttaaac aattgactgt acaagcttcc
      301 catattacgc tagcaagctt gaaacggtac agagccaagc ttatttatct cgctaaccaa
      361 gctttcctac ttacacgtac aagcttgagg tctaaaaact gcaagctttc gaagtaccag
      421 acccaagctt ggcaactcac tgaaccaagc ttgagcgact gtcggtacaa gcttaagcgg
      481 gctcgtccac aagctttgaa cggggatcgg gcaagcttgt cacattaggc ggccaagctt
      541 caatccactg gaatacaagc ttcctctggt tctaactcaa gcttcttaga cgtgtgaagc
      601 aagctttaaa taatcagaac ccaagcttaa gagataaaga agacaagctt agtcacgctg
      661 ggctccaagc ttaatgatgg atgagctcaa gcttgctcga gatctgcgat ctgcatctca
      721 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      781 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
      841 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      901 tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc
      961 tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt
     1021 gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg
     1081 tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa
     1141 catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg
     1201 tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc
     1261 accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc
     1321 acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg
     1381 tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca
     1441 aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca
     1501 aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg
     1561 gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc
     1621 ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc
     1681 tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc
     1741 atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga
     1801 ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca
     1861 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1921 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1981 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2041 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2101 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2161 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2221 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2281 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2341 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2401 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2461 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2521 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg
     2581 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2641 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2701 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2761 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2821 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2881 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2941 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     3001 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct
     3061 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3121 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3181 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     3241 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     3301 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     3361 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     3421 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     3481 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     3541 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     3601 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     3661 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     3721 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     3781 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3841 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     3901 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     3961 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4021 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4081 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4141 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4201 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4261 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     4321 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4381 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4441 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4501 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4561 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4621 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4681 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4741 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4921 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4981 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5041 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5101 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5161 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5221 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5281 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5341 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5401 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5461 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5521 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5581 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5641 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5701 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5761 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5941 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6181 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6241 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6301 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6361 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6421 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
