LOCUS       sequence-258164-        1761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK121
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(539..573)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(644..1303)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1218..1237
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1485..1761,1..312)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
       61 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      361 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct
      421 caaacgggtg aaacaaaacg gttgacaaca tgaagtaaac acggtacgat gtaccacatg
      481 aaacgacagt gagtcaatac agaaacagag gagatattac atatgtgaga ccagaccaat
      541 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      601 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      661 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      721 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      781 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      841 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
      901 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      961 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1021 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1081 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1141 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1201 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1261 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     1321 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1381 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     1441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1741 tcgtgtctta ccgggttgga c
//
