LOCUS       sequence-258174-        7707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 6_pAAV-ProA37-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2021..2030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2027..2953
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2957..2959,2960..2962,2963..3676)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2957..2959,2960..2962,2963..3673)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3002..3023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3263..3282)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3610..3631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3704..4292
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3757..3777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4175..4186)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4324..4800
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4523..4542)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4840..4980
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4840..4969
                     /label=AAV2 ITR
     rep_origin      5055..5510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5142..5161)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5352..5373
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5527..5546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5646..5668
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5706..5724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5792..5896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5897..5965,5966..6757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6115..6134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6928..7516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7417..7436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7578..7707
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtgagccg agttagctct cttacagctg tgtggttcca ttgtcattaa
       61 taagtgcgag tctctcctac ttcctctgtg acttttgctt cttactcatt ttacaagatc
      121 accacgtgtc ttccctgggt tctaagcctt aattggtcac tggtgtgtac agggcacttc
      181 tcaacctgtg ggtcacgatc cttccacagg ggccacatat cagatatcct gcctatcagg
      241 tatttacgat acgattccta acagtagcac tattacaggt atgaagtagc agtgaaaaga
      301 attttatggt tgggggtcgc caccacatga ggagccgtat taggaaggtt gagaacggct
      361 ggtctaaggc atatagctga tgatgtcaaa agacatatca tccatggggg acagctgttg
      421 ctccttccgt cttctccttc gctgggtcct gggcccttgg cttccagcag gctgtgaggt
      481 aaatgtggga tatgaagctg tatcctgagt caattttacc tgacaagcca agggaaggaa
      541 gcagatggtc aggaaggggc cagccctcag gagacttcct tcctccccag ctaggctaca
      601 cctcatcatt tacttcagcc tccccagata gcgccaccag ccggggggac atgcaagcta
      661 gagggtgaca tttcatgccc aaacccaggg aagtgagtcg cccagctagc tgaggccagt
      721 gtgtgctagc gttctgcttg cttctcccac tctccacaca gtggcctctc tacagggcac
      781 ctctacaggg cgtccaactc agccggcagc acattgcctg gcctcggctg gcctccatga
      841 tacctcattc ttgcatttcc acgtgaacat cgagttttgg ggactgcccc ctctgcttta
      901 ggatgctgcc catcagcaga actattctgg tcaaagtgtg atcctaccca tcagaccctc
      961 ccgtgtttca ggcggtctga ggctttgtca gaactctgtg gaatctccct ctgggaggca
     1021 ggctctccct ccgattgatt gatggctccc agccatctcc ttctctcacc ttgagacttg
     1081 ggatggggtg aagtcggggg agtcccagtt tcttggtcca ctgtttcaag agtctgttgg
     1141 ctagagggaa gggcacaagc ttctcttcag agaaatcttt attcctgcag ccaggttcat
     1201 ccccgcactg ccccttggct gtaaaatccc cagcccttgt ttcagtacaa agtccagtct
     1261 ctctgcctta acttagtaga gaaaatgctc tggttagaac agtcttgaat aaatatgaca
     1321 ttttagcagc ttgacacacc aaacaggtgc ctaaggcgcc caaggtacct tgatggtctt
     1381 gtctttgttg ttcaagatgc ggtagccttc ggaagagata cctggcagtg caagtccaca
     1441 ttaggacgca gggaaaagag gggcgtgtgg gtggggggtg cggggggcgg gtgtctacga
     1501 atagctgcag cccaggattg gccctgtaga tcctcaaggc tggtcagatc ttttcccttc
     1561 atatcccagc tggttggaag caccctggct gctgccaaac tgctccccac agaccccaga
     1621 ggggcggctg cccgcagcct ggtttctaat ttcaaaatca cgccacgttc tgcaaacgtt
     1681 gtgtgtttta ggtcaaataa acaactctgc aaacatgtaa ataagctgct ctgcactgat
     1741 tgatcctggg agagggtcag cgagggaggg aaggggaggg agggagggag ggaccgaagg
     1801 gggggaaggc agcggaaagt aggggcaggc ggtgagattc cacagctgag ccaccacctc
     1861 ccgcccaggc cgaatgaaga tgcacaattt ggattggagg ggaagggtca ggagctgctg
     1921 acctttgggc tcaggcccag gccgctggcc acagtagctc ttggtccagt cggcgccgga
     1981 ggtgtatcaa gcgctcatcc cgccctctcc agtcggatcc gccaccatgg attatggagg
     2041 cgccctgagt gccgttgggc gcgagctgct atttgtaacg aacccagtag tcgtcaatgg
     2101 ctctgtactt gtgcctgagg accagtgtta ctgcgcgggc tggattgagt cgcgtggcac
     2161 aaacggtgcc caaacggcgt cgaacgtgct gcaatggctt gctgctggct tctccatcct
     2221 actgcttatg ttttacgcct accaaacatg gaagtcaacc tgcggctggg aggagatcta
     2281 tgtgtgcgct atcgagatgg tcaaggtgat tctcgagttc ttcttcgagt ttaagaaccc
     2341 gtccatgctg tatctagcca caggccaccg cgtccagtgg ttgcgttacg ccgagtggct
     2401 tctcacctgc ccggtcattt gcattcacct gtcaaacctg acgggcttgt ccaacgacta
     2461 cagcaggcgc accatgggtc tgcttgtgtc tgatattggc acaattgtgt ggggcgccac
     2521 ttccgccatg gccaccggat acgtcaaggt catcttcttc tgcctgggtc tgtgttatgg
     2581 tgctaacacg ttctttcacg ctgccaaggc ctacatcgag ggttaccaca ccgtgccgaa
     2641 gggccggtgt cgccaggtgg tgactggcat ggcttggctc ttcttcgtat catggggtat
     2701 gttccccatc ctgttcatcc tcggccccga gggcttcggc gtcctgagcg tgtacggctc
     2761 caccgtcggc cacaccatca ttgacctgat gtcgaagaac tgctggggtc tgctcggcca
     2821 ctacctgcgc gtgctgatcc acgagcatat cctcatccac ggcgacattc gcaagaccac
     2881 caaattgaac attggtggca ctgagattga ggtcgagacg ctggtggagg acgaggccga
     2941 ggctggcgcg gtacccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3001 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3061 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3121 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     3181 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3241 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3301 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3361 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3421 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3481 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3541 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3601 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3661 cgagctgtac aagtgataag aattcgatat caagcttatc gataatcaac ctctggatta
     3721 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3781 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3841 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3901 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3961 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4021 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4081 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     4141 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4201 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4261 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     4321 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     4381 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4441 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4501 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4561 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4621 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4681 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4741 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4801 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     4861 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4921 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     4981 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     5041 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5101 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     5161 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5221 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     5281 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5341 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     5401 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     5461 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     5521 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5581 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5641 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5701 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5761 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5821 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5881 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6001 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6061 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7561 gccttttgct cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     7621 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     7681 tggccaactc catcactagg ggttcct
//
