LOCUS       sequence-258177-        5288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: VII_pAAV-ProB8-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..5288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(755..757,758..760,761..1474)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(755..757,758..760,761..1471)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(800..821)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1061..1080)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1408..1429
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1541..1561)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1959..1970)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2037..2054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2043..2250
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2338..2467
                     /label=AAV2 ITR
     primer_bind     complement(2486..2503)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2486..2502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2495..2517)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2644..3099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2731..2750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2941..2962
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3125..3229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3230..3298,3299..4090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3448..3467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4261..4849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4750..4769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5137..5158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5173..5178,5179..5196,5197..5203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5211..5227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5216..5238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5235..5251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5235..5251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc agacttttcc aacttgccac atcctgagag
      241 atgagccagg acaaagaacc agtaatagct cctggagcag cacatctgtt ttgccaggat
      301 tatcccttgg atctcttaaa accgagacct tgtaatctga agactcaact tgggctgtac
      361 ccttaacctt cagctctatg atgcaagtga gtccacagga ccggaggctt tgagatgagc
      421 ttttcagaag ggaggagttg gccgcttgct cccagagctc cagcacctgc attcttctgg
      481 ctatgtcaga agccagatca tttccctcgt taaaaacaaa aacaaaaaaa caaacaaaca
      541 aaatgttagt ctttgccctt tatctgcctg gcaaagcttt taattggctt gatctgtcat
      601 tccgctagac ataaagggga caatccccgg attaggaagg agctctccag ctcgggtaag
      661 gagtctcaag gcaaggtagg caagcaccac cggtccgcac tctcgcccag cttttacggg
      721 aagaagagag atatccatca cactggcggc cgccatggtg agcaagggcg aggagctgtt
      781 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      841 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      901 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      961 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1021 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1081 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1141 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1201 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1261 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1321 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1381 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1441 gatcactctc ggcatggacg agctgtacaa gtaataagct tggatccaat caacctctgg
     1501 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1561 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1621 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1681 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1741 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     1801 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     1861 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     1921 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     1981 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcga gatctgcctc
     2041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2221 ttgggaagac aatagcaggc atgctgggga ctcgagttaa gggcgaattc ccgataagga
     2281 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     2341 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2401 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2461 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2521 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2581 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2641 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2701 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2761 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2821 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2881 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2941 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3001 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3061 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3121 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3181 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3241 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3301 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3361 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3421 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3481 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3541 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3601 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3661 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3721 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3781 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3841 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3901 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3961 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4021 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4081 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4141 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4201 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4261 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4321 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4381 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4441 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4501 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4561 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4621 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4681 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4741 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4801 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4861 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4921 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4981 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5041 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5101 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5161 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5221 ataacaattt cacacaggaa acagctatga ccatgattac gccagattta attaaggcct
     5281 taattagg
//
