LOCUS       sequence-258188-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for cloning identified individual SPECS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Wu MR, Nissim L, Stupp D, Pery E, Binder-Nissim A, Weisinger K, 
            Enghuus C, Palacios SR, Humphrey M, Zhang Z, Maria Novoa E, Kellis 
            M, Weiss R, Rabkin SD, Tabach Y, Lu TK
  TITLE     A high-throughput screening and computation platform for identifying
            synthetic promoters with enhanced cell-state specificity (SPECS).
  JOURNAL   Nat Commun. 2019 Jun 28;10(1):2880. doi: 10.1038/s41467-019-10912-8.
  PUBMED    31253799
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-28"; volume: "10(1):2880. doi"; pages: " 
            10.1038/s41467-019-10912-8"
COMMENT     Sequence Label: Accessory plasmid 2
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1113..1122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1119..1832
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1932..2049
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2347..2391)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2576..2809
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3302..3427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3474..3654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3672..3870)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3697..3717)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3872..4251
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4423..4442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4517..4621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4622..4690,4691..5482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4840..4859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6142..6161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6395..6412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6529..6550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6565..6570,6571..6588,6589..6595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6603..6619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6608..6630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6627..6643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6627..6643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6692..6813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6703..6722)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6757..6776
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6943..7317)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7336..7383)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7431..7760)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7445..7580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7499..7518)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7745..7765
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7774..8202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7884..7905)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8096..8115
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8248..8472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8461..8478
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8504..8684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg ccctgatgct cttcgtccag atcatcctga
      781 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
      841 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
      901 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
      961 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga ggcgcgccag acgctagcgg
     1021 ggggctataa aagggggtgg gggcgttcgt cctcactcta gatctgcgat ctaagtaagc
     1081 ttggcattcc ggtactgttg gtaaaccagc tcgccaccat ggtgtctaag ggcgaagagc
     1141 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     1201 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     1261 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     1321 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     1381 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     1441 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     1501 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
     1561 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     1621 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg
     1681 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg
     1741 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     1801 tccctagcaa actggggcac aaacttaatt gataaaccgg ttgcagtccc gggtaagcgg
     1861 gactctgggg ttcgaaatga ccgaccaagc attaaccaaa ctggatctct gctgtccctg
     1921 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     1981 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     2041 cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga
     2101 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     2161 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     2221 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     2281 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     2341 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     2401 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     2461 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     2521 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     2581 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     2641 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     2701 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     2761 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     2821 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     2881 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     2941 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     3001 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     3061 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     3121 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     3181 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     3241 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     3301 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     3361 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     3421 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     3481 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     3541 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     3601 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     3661 ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     3721 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     3781 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     3841 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     3901 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     3961 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     4021 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     4081 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     4141 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     4201 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
     4261 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     4321 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     4381 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     4441 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     4501 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4621 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4681 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4741 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4801 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4861 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4921 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4981 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5041 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5101 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5161 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5221 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5281 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5341 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5401 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5461 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5521 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6421 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6481 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6541 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6601 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     6661 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     6721 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6781 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     6841 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     6901 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc
     6961 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     7021 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     7081 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     7141 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     7201 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     7261 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt
     7321 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     7381 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     7441 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     7501 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     7561 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     7621 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     7681 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7741 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
     7801 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7861 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7921 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7981 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8041 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8101 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8161 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     8221 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     8281 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     8341 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     8401 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     8461 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctgctaga gattttccac
     8521 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     8581 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     8641 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca gtccggatgc
     8701 agctctcggg ccatgtgatg aaatgctagg cggctgtcaa acctccactc taatacttct
     8761 ctctccgggt catccatccc atgcaggctc acagggtgta acaagcgggt gttctctcct
     8821 tcattggctt cttctacctt ctcttgctca actggtacta gcttgtagca ccatccaaag
     8881 gtcagtggat atctgatccc tggccctggt gtgtagttct gccaatcagg gaagtagcct
     8941 tgtgtgtggt agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct
     9001 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     9061 ggtcttaaag gtacctgagg tgtgact
//
