LOCUS       sequence-258189-       10359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal HIV-1 packaging plasmid for gag and pol expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Berthoux L, Sebastian S, Sokolskaja E, Luban J
  TITLE     Lv1 inhibition of human immunodeficiency virus type 1 is 
            counteracted by factors that stimulate synthesis or nuclear 
            translocation of viral cDNA.
  JOURNAL   J Virol. 2004 Nov;78(21):11739-50. doi: 
            10.1128/JVI.78.21.11739-11750.2004.
  PUBMED    15479815
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.78.21.11739-11750"; journalName: "J Virol"; date: 
            "2004-11"; volume: "78"; issue: "21"; pages: "11739-50"
COMMENT     Sequence Label: p8.9 NdeltaSB
FEATURES             Location/Qualifiers
     source          1..10359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        226..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        530..733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     684..704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     730..754
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1008..2510
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2303..5314
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4999..5116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5888..6121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6306..6350
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6644..6670
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(7293..7311)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7294..7311)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7601..7620)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7834..7853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7953..7975
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        8099..8203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8204..8272,8273..9064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8422..8441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9235..9823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9724..9743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9977..9994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10064..10084)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10069..10359,1..39)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10249..10359,1..25)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
       61 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
      121 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatcctagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      721 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      781 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg
      841 aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc tatagagtct ataggcccac
      901 ccccttggct tcttatgcga cggatcgatc ccgtaataag cttcgaggtc cgcggccggc
      961 cgcgttgacg cgcacggcaa gaggcgaggg gcggccgctg gtgagagatg ggtgcgagag
     1021 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     1081 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1141 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     1201 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     1261 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     1321 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     1381 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     1441 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
     1501 gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
     1561 ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
     1621 tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg cctattgcac
     1681 caggccagat gagagaacca aggggaagtg acatagcagg aactactagt acccttcagg
     1741 aacaaatagg atggatgaca cataatccac ctatcccagt aggagaaatc tataaaagat
     1801 ggataatcct gggattaaat aaaatagtaa gaatgtatag ccctaccagc attctggaca
     1861 taagacaagg accaaaggaa ccctttagag actatgtaga ccgattctat aaaactctaa
     1921 gagccgagca agcttcacaa gaggtaaaaa attggatgac agaaaccttg ttggtccaaa
     1981 atgcgaaccc agattgtaag actattttaa aagcattggg accaggagcg acactagaag
     2041 aaatgatgac agcatgtcag ggagtggggg gacccggcca taaagcaaga gttttggctg
     2101 aagcaatgag ccaagtaaca aatccagcta ccataatgat acagaaaggc aattttagga
     2161 accaaagaaa gactgttaag tgtttcaatt gtggcaaaga agggcacata gccaaaaatt
     2221 gcagggcccc taggaaaaag ggctgttgga aatgtggaaa ggaaggacac caaatgaaag
     2281 attgtactga gagacaggct aattttttag ggaagatctg gccttcccac aagggaaggc
     2341 cagggaattt tcttcagagc agaccagagc caacagcccc accagaagag agcttcaggt
     2401 ttggggaaga gacaacaact ccctctcaga agcaggagcc gatagacaag gaactgtatc
     2461 ctttagcttc cctcagatca ctctttggca gcgacccctc gtcacaataa agataggggg
     2521 gcaattaaag gaagctctat tagatacagg agcagatgat acagtattag aagaaatgaa
     2581 tttgccagga agatggaaac caaaaatgat agggggaatt ggaggtttta tcaaagtaag
     2641 acagtatgat cagatactca tagaaatctg cggacataaa gctataggta cagtattagt
     2701 aggacctaca cctgtcaaca taattggaag aaatctgttg actcagattg gctgcacttt
     2761 aaattttccc attagtccta ttgagactgt accagtaaaa ttaaagccag gaatggatgg
     2821 cccaaaagtt aaacaatggc cattgacaga agaaaaaata aaagcattag tagaaatttg
     2881 tacagaaatg gaaaaggaag gaaaaatttc aaaaattggg cctgaaaatc catacaatac
     2941 tccagtattt gccataaaga aaaaagacag tactaaatgg agaaaattag tagatttcag
     3001 agaacttaat aagagaactc aagatttctg ggaagttcaa ttaggaatac cacatcctgc
     3061 agggttaaaa cagaaaaaat cagtaacagt actggatgtg ggcgatgcat atttttcagt
     3121 tcccttagat aaagacttca ggaagtatac tgcatttacc atacctagta taaacaatga
     3181 gacaccaggg attagatatc agtacaatgt gcttccacag ggatggaaag gatcaccagc
     3241 aatattccag tgtagcatga caaaaatctt agagcctttt agaaaacaaa atccagacat
     3301 agtcatctat caatacatgg atgatttgta tgtaggatct gacttagaaa tagggcagca
     3361 tagaacaaaa atagaggaac tgagacaaca tctgttgagg tggggattta ccacaccaga
     3421 caaaaaacat cagaaagaac ctccattcct ttggatgggt tatgaactcc atcctgataa
     3481 atggacagta cagcctatag tgctgccaga aaaggacagc tggactgtca atgacataca
     3541 gaaattagtg ggaaaattga attgggcaag tcagatttat gcagggatta aagtaaggca
     3601 attatgtaaa cttcttaggg gaaccaaagc actaacagaa gtagtaccac taacagaaga
     3661 agcagagcta gaactggcag aaaacaggga gattctaaaa gaaccggtac atggagtgta
     3721 ttatgaccca tcaaaagact taatagcaga aatacagaag caggggcaag gccaatggac
     3781 atatcaaatt tatcaagagc catttaaaaa tctgaaaaca ggaaagtatg caagaatgaa
     3841 gggtgcccac actaatgatg tgaaacaatt aacagaggca gtacaaaaaa tagccacaga
     3901 aagcatagta atatggggaa agactcctaa atttaaatta cccatacaaa aggaaacatg
     3961 ggaagcatgg tggacagagt attggcaagc cacctggatt cctgagtggg agtttgtcaa
     4021 tacccctccc ttagtgaagt tatggtacca gttagagaaa gaacccataa taggagcaga
     4081 aactttctat gtagatgggg cagccaatag ggaaactaaa ttaggaaaag caggatatgt
     4141 aactgacaga ggaagacaaa aagttgtccc cctaacggac acaacaaatc agaagactga
     4201 gttacaagca attcatctag ctttgcagga ttcgggatta gaagtaaaca tagtgacaga
     4261 ctcacaatat gcattgggaa tcattcaagc acaaccagat aagagtgaat cagagttagt
     4321 cagtcaaata atagagcagt taataaaaaa ggaaaaagtc tacctggcat gggtaccagc
     4381 acacaaagga attggaggaa atgaacaagt agataaattg gtcagtgctg gaatcaggaa
     4441 agtactattt ttagatggaa tagataaggc ccaagaagaa catgagaaat atcacagtaa
     4501 ttggagagca atggctagtg attttaacct accacctgta gtagcaaaag aaatagtagc
     4561 cagctgtgat aaatgtcagc taaaagggga agccatgcat ggacaagtag actgtagccc
     4621 aggaatatgg cagctagatt gtacacattt agaaggaaaa gttatcttgg tagcagttca
     4681 tgtagccagt ggatatatag aagcagaagt aattccagca gagacagggc aagaaacagc
     4741 atacttcctc ttaaaattag caggaagatg gccagtaaaa acagtacata cagacaatgg
     4801 cagcaatttc accagtacta cagttaaggc cgcctgttgg tgggcgggga tcaagcagga
     4861 atttggcatt ccctacaatc cccaaagtca aggagtaata gaatctatga ataaagaatt
     4921 aaagaaaatt ataggacagg taagagatca ggctgaacat cttaagacag cagtacaaat
     4981 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     5041 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     5101 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggaaaggacc
     5161 agcaaagctc ctctggaaag gtgaaggggc agtagtaata caagataata gtgacataaa
     5221 agtagtgcca agaagaaaag caaagatcat cagggattat ggaaaacaga tggcaggtga
     5281 tgattgtgtg gcaagtagac aggatgagga ttaacacatg gaattctgca acaactgctg
     5341 tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactcg acagaggaga
     5401 gcaagaaatg gagccagtag atcctagact agagccctgg aagcatccag gaagtcagcc
     5461 taaaactgct tgtaccaatt gctattgtaa aaagtgttgc tttcattgcc aagtttgttt
     5521 catgacaaaa gccttaggca tctcctatgg caggaagaag cggagacagc gacgaagagc
     5581 tcatcagaac agtcagactc atcaagcttc tctatcaaag cagtaagtag tacatgtaat
     5641 gcaacctata atagtagcaa tagtagcatt agtagtagca ataataatag caatagttgt
     5701 gtggtccata gtaatcatag aatataggaa aatggccgct gatcttcaga cctggaggag
     5761 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     5821 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     5881 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     5941 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6001 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     6061 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     6121 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     6181 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     6241 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     6301 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     6361 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     6421 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     6481 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     6541 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcct
     6601 tggcacttat ctgggacgat ctgcggagcc tgtgcctctt cagctaccac cgcttgagag
     6661 acttactctt gattgtaacg aggattgtgg aacttctggg acgcaggggg tgggaagccc
     6721 tcaaatattg gtggaatctc ctacaatatt ggagtcagga gctaaagaat agtgctgtta
     6781 gcttgctcaa tgccacagcc atagcagtag ctgaggggac agatagggtt atagaagtag
     6841 tacaaggagc ttgtagagct attcgccaca tacctagaag aataagacag ggcttggaaa
     6901 ggattttgct ataagctcga ggccgccccg gtgaccttca gaccttggca ctggaggtgg
     6961 cccggcagaa gcgcggcatc gtggatcagt gctgcaccag catctgctct ctctaccaac
     7021 tggagaacta ctgcaactag gcccaccact accctgtcca cccctctgca atgaataaaa
     7081 cctttgaaag agcactacaa gttgtgtgta catgcgtgca tgtgcatatg tggtgcgggg
     7141 ggaacatgag tggggctggc tggagtggcg atgataagct gtcaaacatg agaattaatt
     7201 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7261 tggtttctta gtctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     7321 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     7381 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     7441 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     7501 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     7561 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     7621 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     7681 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     7741 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     7801 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7861 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7921 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7981 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     8041 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9121 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9361 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9901 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9961 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    10021 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
    10081 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10141 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10201 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10261 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10321 gaggccgagg ccgcctcggc ctctgagcta ttccagaag
//
