LOCUS       sequence-25819-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pDUET-ctCPR-sAMO
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..93
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cntgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc
       61 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      121 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atggcgctgg ataaactgga
      181 tctgtatgtg atcattacgc tggtagttgc ggttgctgct tatttcgcga agaaccagtt
      241 tctggaccag ccgcaggaca ccggcttcct gaacaccgat tctggcagca actctcgtga
      301 cgtactgtct accctgaaga agaacaacaa gaacactctg ctgctgttcg gcagccagac
      361 tggcaccgct gaggattacg cgaacaaact gtctcgtgaa ctgcattccc gcttcggtct
      421 gaagactatg gttgcggatt tcgcagacta cgactgggac aatttcggcg acatcaccga
      481 agacatcctg gtgttcttta tcgtggccac gtacggcgaa ggcgaaccga ccgataacgc
      541 agatgaattt cacacgtggc tgacggagga ggctgacacc ctgtccaccc tgaagtacac
      601 cgttttcggt ctgggtaaca gcacctatga gtctcctnca acgcctatcg gtcgtaaatt
      661 tgaccgtctg ctgtctgaaa nggtgggtga tcgtttccgc gnnntatgct gaagngatga
      721 cggtactggt actctggatg aagatttcat ggcttgaaaa gacaacgttt tcgatgcact
      781 gaagaacgat ctgaatttcg aggaaaaaag aactgaagtt
//
