LOCUS       sequence-258197-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of an inverse-response citrate sensor variant 
            with medium binding affinity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Zhao Y, Shen Y, Wen Y, Campbell RE
  TITLE     High-Performance Intensiometric Direct- and Inverse-Response 
            Genetically Encoded Biosensors for Citrate.
  JOURNAL   ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. doi: 
            10.1021/acscentsci.0c00518. Epub 2020 Jul 9.
  PUBMED    32875085
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.0c00518"; journalName: "ACS Cent Sci"; date: 
            "2020-08-26- 26"; volume: "6"; issue: "8"; pages: "1441-1450"
COMMENT     Sequence Label: pBAD-Citroff1-S245
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1044..1065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactggca gaccgaggag cgtctgcatt atcaggtcgg gcaacgggcg
      481 ctgattcagg cgatgcagat ttcggcgatg ccggagctgg tggaggcagt gcagaaacgc
      541 gatctcgcca gaatcaaagc ccttatcggc cccatgcgtt cgttctccga cgccacctac
      601 atcaccgtcg gtgatgccag cggccagcgc ctctatcacg tcaatcctga tgaaatcggc
      661 aaatcgatgg aaggcggcga tagcgatgag gcgttgataa acgctaaaag ctacgtgtca
      721 gtgcgcaaag gcgcgctggg atcgtcgctg cgcggtaaat cgccgattca ggacgcaacc
      781 ggcaaggtga tcggcattgt gtcggtaggc tataccattg agcaactgga accgaatttt
      841 gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcca catccgccac
      901 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
      961 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccca actttcgaaa
     1021 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1081 actctcggca tggtcgagct gtacaagtaa
//
