LOCUS       sequence-25820-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pDUET-ctCPR-trAMO
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             130..152
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tnnngcgcng gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga
       61 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      121 agaaataatt ttgtttaact ttaagaagga gatataccat ggcgctggat aaactggatc
      181 tgtatgtgat cattacgctg gtagttgcgg ttgctgctta tttcgcgaag aaccagtttc
      241 tggaccagcc gcaggacacc ggcttcctga acaccgattc tggcagcaac tctcgtgacg
      301 tactgtctac cctgaagaag aacaacaaga acactctgct gctgttcggc agccagactg
      361 gcaccgctga ggattacgcg aacaaactgt ctcgtgaact gcattcccgc ttcggtctga
      421 agactatggt tgcggatttc gcagactacg actgggacaa tttcggcgac atcaccgaag
      481 acatcctggt gttctttatc gtggccacgt acggcgaagg cgaaccgacc gataacgcag
      541 atgaatttca cacgtggctg acggaggagg ctgacaccct gtccaccctg aagtacaccg
      601 ttttcggtct gggtaacagc acctatgagt tcttcaacgc tatcggtcgt aaatttgacc
      661 gtctgctgtc tgaaaaaggg tggtgatcgt tnncgcggna ttnnngctnn nnngnnnaga
      721 nnnactgtac ctgnnnancc tgggatgaag gatttcatgg gcttggaaaa gacaacgttt
      781 ttcgtatgca ctgaaagaac cgatctgaaa ttttc
//
