LOCUS       sequence-25822-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pDUET-ctCPR-BovAMO
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    92..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..153
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctgnggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
       61 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      121 tagaaataat tttgtttaac tttaagaagg agatatacca tggcgctgga taaactggat
      181 ctgtatgtga tcattacgct ggtagttgcg gttgctgctt atttcgcgaa gaaccagttt
      241 ctggaccagc cgcaggacac cggcttcctg aacaccgatt ctggcagcaa ctctcgtgac
      301 gtactgtcta ccctgaagaa gaacaacaag aacactctgc tgctgttcgg cagccagact
      361 ggcaccgctg aggattacgc gaacaaactg tctcgtgaac tgcattcccg cttcggtctg
      421 aagactatgg ttgcggattt cgcagactac gactgggaca atttcggcga catcaccgaa
      481 gacatcctgg tgttctttat cgtggccacg tacggcgaag gcgaaccgac cgataacgca
      541 gatgaatttc acacgtggct gacggaggag gctgacaccc tgtccaccct gaagtacacc
      601 gttttcggtc tgggtaacag cacctatgag ttcttcaacg ctatcggtcg taaatttgac
      661 cgtctgctgt ctgaaaaggg tggtgatcgt ttcgcgnnnn nnntnnnnna nggcaaaaac
      721 ggtnnnngtt nnnnnnanag aaaaatttca tggccttgga aaagacnacg tttttcnnnn
      781 tgcnnctgaa annaacnaan tctnnanttt tcgagnaaaa agaactgaag tac
//
