LOCUS       sequence-258221-       10546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac 
            trasposon plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG
  TITLE     Ultrasound imaging of gene expression in mammalian cells.
  JOURNAL   Science. 2019 Sep 27;365(6460):1469-1475. doi: 
            10.1126/science.aax4804.
  PUBMED    31604277
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1469-1475"
COMMENT     Sequence Label: mARG1 cassette 2
FEATURES             Location/Qualifiers
     source          1..10546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1370..1719
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1414..1432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1450..1468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1486..1504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1522..1540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1558..1576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1594..1612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1658..1682
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2681..2737
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2864..2873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2868..2877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3521..3577
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3860..3916
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4742..4798
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5102..5158
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5459..5515
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5834..5890
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6323..6379
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6686..6705)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7116..7237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7153..7172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7207..7226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8000..8034
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8525..8541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8525..8541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8538..8560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8549..8565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8573..8579,8580..8597,8598..8603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8618..8639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8756..8773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8927..9515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9007..9026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9686..10477,10478..10546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10309..10328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtttcactcg agtttactcc
     1381 ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc
     1441 agactttact ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata
     1501 gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc
     1561 ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga gaacgtatgt
     1621 cgaggtaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga
     1681 tcgcctggag caattccaca acacttttgt cttatacttg gtacctatgc atgccaccat
     1741 gaccgtgctg accgacaagc ggaagaaggg cagcggcgcc ttcatccagg acgacgagac
     1801 aaaagaggtg ctgagcagag ccctgagcta cctgaagtcc ggctacagca tccacttcac
     1861 cggacctgcc ggcggaggca agacatctct ggctagagcc ctggccaaga aacggaagcg
     1921 gcccgtgatg ctgatgcacg gcaaccacga gctgaacaac aaggacctga tcggcgattt
     1981 caccggctac accagcaaaa aggtgatcga ccagtacgtg cggagcgtgt acaagaaaga
     2041 cgaacaggtg tccgagaact ggcaggacgg cagactgctg gaagccgtga agaatggcta
     2101 caccctgatc tacgacgagt tcaccagaag caagcccgct accaacaaca tcttcctgag
     2161 catccttgag gagggcgtgc tgcccctgta cggcgtgaag atgaccgacc ctttcgtgcg
     2221 cgtgcacccc gacttcagag tgatctttac cagcaacccc gccgagtatg ccggcgtgta
     2281 cgatacccag gacgccctgc tggaccggct gatcaccatg ttcatcgact acaaggacat
     2341 cgaccgggaa accgctatcc tgaccgagaa aactgacgtg gaagaagacg aggcccggac
     2401 catcgtgacc ctggtggcca acgtgcggaa cagaagcggc gacgagaata gcagcggcct
     2461 gagcctgaga gccagcctga tgattgccac cctggccacc cagcaggaca tccctatcga
     2521 tggcagcgac gaggacttcc agaccctgtg catcgacatc ctgcaccacc ccctgaccaa
     2581 gtgcctggac gaagagaacg ccaagagcaa ggccgagaag atcattctcg aagagtgcaa
     2641 gaacatcgac accgaggaga agggtgcccc gggatctggc gcaacaaatt ttagtctttt
     2701 aaagcaggca ggagacgtcg aggaaaaccc tggacccgtg agcgagacaa acgagacagg
     2761 catctacatc ttcagcgcca tccagacaga caaggatgag gaattcggcg ccgtggaagt
     2821 ggaagggacc aaggctgaga cattcctgat ccggtataag gacgccgcca tggtggccgc
     2881 cgaagtgccc atgaagatct accaccccaa ccggcagaac ctgctgatgc accagaatgc
     2941 cgtggccgcc atcatggaca agaacgacac cgtgatcccc atcagcttcg gcaacgtgtt
     3001 caagagcaaa gaggacgtga aggtgctcct ggaaaacctg tacccccagt tcgagaagct
     3061 gttccccgcc atcaagggaa agatcgaagt gggcctgaag gtgatcggca agaaagagtg
     3121 gctcgaaaag aaagtgaacg agaaccccga gctggaaaaa gtgtccgcca gcgtgaaggg
     3181 caagagcgag gccgctggct actacgagag aatccagctg ggcggcatgg cccagaagat
     3241 gttcacaagc ctgcagaaag aagtgaaaac cgacgtgttc agccccctgg aagaagccgc
     3301 cgaggccgcc aaagccaatg agcctacagg cgaaacaatg ctgctgaacg ccagcttcct
     3361 gatcaacaga gaggatgagg ccaagttcga cgagaaagtc aatgaggccc acgagaactg
     3421 gaaggataag gccgacttcc actacagcgg cccctggccc gcctacaact tcgtgaacat
     3481 ccggctgaag gtggaagaga agggggcacc tggctcggga gcgaccaact tctcattact
     3541 caaacaagcc ggagacgttg aggagaatcc aggccctgtg ctgcacaagc tcgtgaccgc
     3601 ccccatcaac ctggtcgtga agatcggcga gaaggtgcag gaagaggccg acaagcagct
     3661 gtacgacctg cccaccatcc agcagaagct gatccagctg cagatgatgt tcgagctggg
     3721 cgagatcccc gaggaagcct tccaggaaaa agaggacgaa ctgctgatga gatacgagat
     3781 cgccaagcgg cgcgagattg agcagtggga agaactgacc cagaagcgga atgaggaaag
     3841 cggtgccccg ggatctggcg caacaaattt tagtctttta aagcaggcag gagacgtcga
     3901 ggaaaaccct ggacccgtgg gcgagctgct gtacctctac ggcctgatcc ccaccaaaga
     3961 ggccgctgct atcgagccct tcccattcta caagggcttc gacggcgagc acagcctgta
     4021 ccctatcgcc ttcgaccaag tgaccgccgt ggtgttcaag ctggacgccg acacctacag
     4081 cgagaaagtg atccaggaaa agatggaaca ggacatgagc tggctgcagg aaaaggcctt
     4141 ccaccaccac gagacagtgg ccgccctgta tgaggaattc accatcatcc ccctgaagtt
     4201 ctgcaccatc tataagggag aggaatccct gcaggccgcc atcgagatca acaaagagaa
     4261 gatcgaaaac tccctgaccc tgctgcaggg caacgaggaa tggaacgtga agatctactg
     4321 cgacgacacc gagctgaaga agggcatcag cgagacaaac gagagcgtga aggccaagaa
     4381 gcaggaaatc agccacctga gccccggcag acagttcttc gagaagaaga agattgacca
     4441 gctcatcgag aaagagctgg aactgcacaa gaacaaagtg tgcgaggaaa tccacgacaa
     4501 gctgattgag ctgagcctct acgactccgt gaagaagaac tggtccaagg acgtgacagg
     4561 cgctgccgaa cagatggcct ggaacagcgt gttcctgctg cccagcctgc agatcaccaa
     4621 gttcgtgaac gagatcgagg aactccagca gcggctggag aacaagggat ggaagttcga
     4681 agtgaccggc ccctggcctc cctaccactt cagcagcttt gccggggcac ctggctcggg
     4741 agcgaccaac ttctcattac tcaaacaagc cggagacgtt gaggagaatc caggccctgt
     4801 gagcctgaag cagagcatgg agaataagga tatcgccctg atcgacatcc tcgacgtgat
     4861 cctggacaag ggagtggcca tcaagggcga cctgatcatc tctatcgccg gcgtggacct
     4921 ggtgtacctg gatctgagag tgctgatctc cagcgtggaa accctggtgc aggccaaaga
     4981 gggcaaccac aagcccatca ccagcgagca gttcgacaag cagaaagagg agctgatgga
     5041 cgccaccggc cagcccagca agtggacaaa tcctctgggc agcggcgctc ccgggtcagg
     5101 tgccacgaat ttttcgttgt tgaagcaagc tggggatgtt gaagagaacc cagggcctgt
     5161 gcagcccgtg tcccaggcca acggcagaat ccacctggat cccgatcagg ccgaacaggg
     5221 actggcccag ctcgtgatga ccgtgatcga gctgctgcgg cagatcgtgg aacggcacgc
     5281 catgagaaga gtggaaggcg gcaccctgac cgacgagcag atcgagaatc tgggaatcgc
     5341 tctgatgaac ctggaggaga agatggacga gctgaaagag gtgttcggac tggacgctga
     5401 ggatctgaac atcgacctgg gccctctggg cagcctgctg ggtgccccgg gatctggcgc
     5461 aacaaatttt agtcttttaa agcaggcagg agacgtcgag gaaaaccctg gacccgtggc
     5521 cgtggaacac aacatgcaga gcagcaccat cgtggacgtg ctggaaaaga tcctggacaa
     5581 gggcgtcgtg atcgccgggg acatcacagt gggaatcgcc gacgtggaac tgctgaccat
     5641 caagatccgg ctgatcgtgg ccagcgtgga caaggccaaa gaaatcggca tggattggtg
     5701 ggagaacgac ccctacctga gcagcaaggg cgccaacaac aaggctctgg aagaggaaaa
     5761 caagatgctg cacgagcggc tgaaaacact ggaagagaag atcgagacaa agcgcggggc
     5821 acctggctcg ggagcgacca acttctcatt actcaaacaa gccggagacg ttgaggagaa
     5881 tccaggccct gtgagcaccg gccccagctt cagcaccaag gacaacaccc tggaatactt
     5941 cgtgaaggcc agcaacaagc acggctttag cctcgacatc agcctgaacg tgaatggggc
     6001 cgtgattagc ggcaccatga tcagcgccaa agagtacttc gactacctga gcgagacatt
     6061 cgaagagggc agcgaagtgg cccaggccct gtctgagcag tttagcctgg ctagcgaggc
     6121 ctccgagtct aatggcgaag ccgaggccca cttcatccac ctgaagaaca ccaagatcta
     6181 ctgcggcgac agcaagagca cccccagcaa gggcaagatc ttctggcgcg gcaagatcgc
     6241 cgaggtggac ggattcttcc tgggaaaaat cagcgacgcc aagtccacca gcaagaagtc
     6301 cagcggcgct cccgggtcag gtgccacgaa tttttcgttg ttgaagcaag ctggggatgt
     6361 tgaagagaac ccagggcctg tggtgtccaa gggcgaggaa ctgttcaccg gcgtggtgcc
     6421 catcctggtg gaactggatg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg
     6481 cgaaggcgac gccacatacg gaaagctgac cctgaagttc atctgcacca ccggcaagct
     6541 gcccgtgcct tggcctaccc tcgtgaccac actgacctac ggcgtgcagt gcttcgccag
     6601 ataccccgac cacatgaagc agcacgattt cttcaagagc gccatgcccg agggctacgt
     6661 gcaggaacgg accatcttct tcaaggacga cggcaactac aagacaagag ccgaagtgaa
     6721 gttcgagggc gacaccctcg tgaaccggat cgagctgaag ggcatcgact tcaaagagga
     6781 tggcaacatc ctgggccaca agctggagta caactacaac agccacaagg tgtacatcac
     6841 cgccgacaag cagaaaaacg gcatcaaagt gaacttcaag acccggcaca acatcgagga
     6901 cggcagcgtg cagctggccg accactacca gcagaacacc cccatcggag atggccccgt
     6961 gctgctgccc gacaaccact acctgagcac acaaagcgcc ctgagcaagg accccaacga
     7021 gaagcgggac cacatggtgc tgctggaatt tgtgaccgcc gctggcatca ccctgggcat
     7081 ggacgagctg tacaagtgaa ctagttcgtt aactaaactt gtttattgca gcttataatg
     7141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7201 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
     7261 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
     7321 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
     7381 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
     7441 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     7501 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     7561 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     7621 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     7681 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
     7741 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     7801 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     7861 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     7921 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     7981 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     8041 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     8101 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     8161 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     8221 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     8281 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     8341 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     8401 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     8461 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     8521 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8581 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8641 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9361 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcat
//
