LOCUS       sequence-258224-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac 
            trasposon plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG
  TITLE     Ultrasound imaging of gene expression in mammalian cells.
  JOURNAL   Science. 2019 Sep 27;365(6460):1469-1475. doi: 
            10.1126/science.aax4804.
  PUBMED    31604277
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1469-1475"
COMMENT     Sequence Label: mARG1 cassette 3
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1391..1472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2362..2380)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2363..2380)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2453..2802
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2497..2515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2533..2551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2569..2587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2605..2623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2641..2659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      2816..2825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3140..3196
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      3323..3332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3327..3336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3980..4036
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4319..4375
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5201..5257
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    5572..5693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5663..5682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6456..6490
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6981..6997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6981..6997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6994..7016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7005..7021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7029..7035,7036..7053,7054..7059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7074..7095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7212..7229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7383..7971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7463..7482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8142..8933,8934..9002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8765..8784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gttttccccg aaaagtgcca
     1381 cctgacgtcg gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1441 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1501 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1561 gtatggctga ttatgatcct ctagacatat gctgcagtca cttgtacagc tcatccatgc
     1621 ccagggtgat gccagcggcg gtccgaaatt ccagcagcac catgtggtcc cgcttctcgt
     1681 tggggtcctt gctcagcacg ctctgggtgc tcaggtagtg gctatcaggc agcagcacgg
     1741 ggccatctcc gatgggggtg ttctgctggt agtggtcggc cagctgcacg ctgccatctt
     1801 ccacgttgtg ccggatcttg aagttcactt tgatgccgtt tttctgcttc acggccatga
     1861 tgtagatgtt gtggctgttg aagttgtact ccagcttgtg gcccaggatg ttgccgtcct
     1921 ctttgaagtc cacgcccttc agctcgatcc ggttcacgag ggtgtcgccc tcgaacttca
     1981 cttcggctct ggtcttgtag gtgccgtcgt ccttgaagaa gatggtccgt tcctgcacgt
     2041 agccctcggg catggcgctc ttgaagaaat cgtgctgctt catgtggtcg gggtatctgg
     2101 cgaagcactg cacgccgtga gacagtgtgg tcacgagggt aggccaaggc acgggcagct
     2161 tgccggtggt gcagatgaac ttcagggtca gcttgccatt tgtggcgtcg ccttcgccct
     2221 ctccccgcac agagaacttg tggccgttca cgtcgccatc cagttccacc aggatgggca
     2281 ccacgccggt gaacagttcc tcgcccttgg acaccatggt gaagggtact ggatccgagc
     2341 tcggtacctg caggcgtacc ttctatagtg tcacctaaat gcgatctgac ggttcactaa
     2401 acgagctctg cttatatagg cctcccaccg tacacgccac ctcgacatac tcgagtttac
     2461 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
     2521 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
     2581 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
     2641 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
     2701 tgtcgaggta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagcaattcc acaacacttt tgtcttatac ttggtaccta tgcatgccac
     2821 catggccgtg gaacacaaca tgcagagcag caccatcgtg gacgtgctgg aaaagatcct
     2881 ggacaagggc gtcgtgatcg ccggggacat cacagtggga atcgccgacg tggaactgct
     2941 gaccatcaag atccggctga tcgtggccag cgtggacaag gccaaagaaa tcggcatgga
     3001 ttggtgggag aacgacccct acctgagcag caagggcgcc aacaacaagg ccctggaaga
     3061 ggaaaacaag atgctgcacg agcggctgaa aacactggaa gagaagatcg agacaaagcg
     3121 cggtgccccg ggatctggcg caacaaattt tagtctttta aagcaggcag gagacgtcga
     3181 ggaaaaccct ggacccgtga gcgagacaaa cgagacaggc atctacatct tcagcgccat
     3241 ccagacagac aaggatgagg aattcggcgc cgtggaagtg gaagggacca aggctgagac
     3301 attcctgatc cggtataagg acgccgccat ggtggccgcc gaagtgccca tgaagatcta
     3361 ccaccccaac cggcagaacc tgctgatgca ccagaatgcc gtggccgcca tcatggacaa
     3421 gaacgacacc gtgatcccca tcagcttcgg caacgtgttc aagagcaaag aggacgtgaa
     3481 ggtgctcctg gaaaacctgt acccccagtt cgagaagctg ttccccgcca tcaagggaaa
     3541 gatcgaagtg ggcctgaagg tgatcggcaa gaaagagtgg ctcgaaaaga aagtgaacga
     3601 gaaccccgag ctggaaaaag tgtccgccag cgtgaagggc aagagcgagg ccgctggcta
     3661 ctacgagaga atccagctgg gcggcatggc ccagaagatg ttcacaagcc tgcagaaaga
     3721 agtgaaaacc gacgtgttca gccccctgga agaagccgcc gaggccgcca aagccaatga
     3781 gcctacaggc gaaacaatgc tgctgaacgc cagcttcctg atcaacagag aggatgaggc
     3841 caagttcgac gagaaagtca atgaggccca cgagaactgg aaggataagg ccgacttcca
     3901 ctacagcggc ccctggcccg cctacaactt cgtgaacatc cggctgaagg tggaagagaa
     3961 gggggcacct ggctcgggag cgaccaactt ctcattactc aaacaagccg gagacgttga
     4021 ggagaatcca ggccctgtgc tgcacaagct cgtgaccgcc cccatcaacc tggtcgtgaa
     4081 gatcggcgag aaggtgcagg aagaggccga caagcagctg tacgacctgc ccaccatcca
     4141 gcagaagctg atccagctgc agatgatgtt cgagctgggc gagatccccg aggaagcctt
     4201 ccaggaaaaa gaggacgaac tgctgatgag atacgagatc gccaagcggc gcgagattga
     4261 gcagtgggaa gaactgaccc agaagcggaa tgaggaaagc ggtgccccgg gatctggcgc
     4321 aacaaatttt agtcttttaa agcaggcagg agacgtcgag gaaaaccctg gacccgtggg
     4381 cgagctgctg tacctctacg gcctgatccc caccaaagag gccgctgcta tcgagccctt
     4441 cccattctac aagggcttcg acggcgagca cagcctgtac cctatcgcct tcgaccaagt
     4501 gaccgccgtg gtgttcaagc tggacgccga cacctacagc gagaaagtga tccaggaaaa
     4561 gatggaacag gacatgagct ggctgcagga aaaggccttc caccaccacg agacagtggc
     4621 cgccctgtat gaggaattca ccatcatccc cctgaagttc tgcaccatct ataagggaga
     4681 ggaatccctg caggccgcca tcgagatcaa caaagagaag atcgaaaact ccctgaccct
     4741 gctgcagggc aacgaggaat ggaacgtgaa gatctactgc gacgacaccg agctgaagaa
     4801 gggcatcagc gagacaaacg agagcgtgaa ggccaagaag caggaaatca gccacctgag
     4861 ccccggcaga cagttcttcg agaagaagaa gattgaccag ctcatcgaga aagagctgga
     4921 actgcacaag aacaaagtgt gcgaggaaat ccacgacaag ctgattgagc tgagcctcta
     4981 cgactccgtg aagaagaact ggtccaagga cgtgacaggc gctgccgaac agatggcctg
     5041 gaacagcgtg ttcctgctgc ccagcctgca gatcaccaag ttcgtgaacg agatcgagga
     5101 actccagcag cggctggaga acaagggatg gaagttcgaa gtgaccggcc cctggcctcc
     5161 ctaccacttc agcagctttg ccggggcacc tggctcggga gcgaccaact tctcattact
     5221 caaacaagcc ggagacgttg aggagaatcc aggccctgtg cagcccgtgt cccaggccaa
     5281 cggcagaatc cacctggatc ccgatcaggc cgaacaggga ctggcccagc tcgtgatgac
     5341 cgtgatcgag ctgctgcggc agatcgtgga acggcacgcc atgagaagag tggaaggcgg
     5401 caccctgacc gacgagcaga tcgagaatct gggaatcgcc ctgatgaacc tggaagagaa
     5461 gatggacgag ctgaaagagg tgttcggact ggacgccgag gacctgaaca tcgacctggg
     5521 ccctctgggc agcctgctgt gaactagttc gataccgtcg accgttaact aaacttgttt
     5581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5701 tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc tcccttccgg
     5761 gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg gttcttatat
     5821 cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg aagtccctaa
     5881 agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt tagggcccat
     5941 tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     6001 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     6061 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     6121 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     6181 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     6241 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     6301 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     6361 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     6421 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     6481 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     6541 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     6601 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     6661 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     6721 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     6781 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     6841 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     6901 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     6961 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7021 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7801 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8281 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9001 at
//
