LOCUS       sequence-25824-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pDUET-ctCPR-A13AMO
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgcggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat accatggcgc tggataaact ggatctgtat
      181 gtgatcatta cgctggtagt tgcggttgct gcttatttcg cgaagaacca gtttctggac
      241 cagccgcagg acaccggctt cctgaacacc gattctggca gcaactctcg tgacgtactg
      301 tctaccctga agaagaacaa caagaacact ctgctgctgt tcggcagcca gactggcacc
      361 gctgaggatt acgcgaacaa actgtctcgt gaactgcatt cccgcttcgg tctgaagact
      421 atggttgcgg atttcgcaga ctacgactgg gacaatttcg gcgacatcac cgaagacatc
      481 ctggtgttct ttatcgtggc cacgtacggc gaaggcgaac cgaccgataa cgcagatgaa
      541 tttcacacgt ggctgacgga ggaggctgac accctgtcca ccctgaagta caccgttttc
      601 ggtctgggta acagcaccta tgagttcttc aacgctatcg gtcgtaaatt tgaccgtctg
      661 ctgtctgaaa agggtggtga tcgtttcgcg gaatatgctg aaggcgatga cggtactggt
      721 actctggatg aagatttcat ggcttggaaa ganacttntt ttttnnnngc acttgaaaaa
      781 cgatcctgaa tttcgagagn a
//
