LOCUS       sequence-258248-        6597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma Targeted, High photocurrent, low-light sensitive 
            channelrhodopsin (ChRger2) for optogenetic activation with systemic 
            delivery or for low-light activation. Driven by human Synapsin I 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH
  TITLE     Machine learning-guided channelrhodopsin engineering enables 
            minimally invasive optogenetics.
  JOURNAL   Nat Methods. 2019 Oct 14. pii: 10.1038/s41592-019-0583-8. doi: 
            10.1038/s41592-019-0583-8.
  PUBMED    31611694
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Oct 14. pii: 10.1038/s41592-019-0583-8. doi: 
            10.1038/s41592-019-0583-8."
COMMENT     Sequence Label: pAAV-hSyn-STChRger2-TS-EYFP
FEATURES             Location/Qualifiers
     source          1..6597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             723..761
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             join(1980..1982,1983..2696)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2022..2043)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2283..2302)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2630..2651
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2724..3312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2777..2797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3195..3206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3344..3820
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3543..3562)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3860..4000
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3860..3989
                     /label=AAV2 ITR
     rep_origin      4075..4530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4162..4181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4372..4393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4547..4566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4666..4688
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4726..4744)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4812..4916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4917..4985,4986..5777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5135..5154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5948..6536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6437..6456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgccaccatg agcagactgg
      661 tcgccgcttc ttggctgctg gctctcctcc tctgcggaat taccagcaca acaacagcct
      721 ctgcccacat cgtgatggtg gacgcctaca agcccaccaa gagcgcccca gcagcttctt
      781 ctacagacgg aacagccgcc gcagcagtgt ctcactacgc catgaacggc ttcgacgagc
      841 tggctaaagg agccgtggtg ccagaagacc actttgtctg cggaccagcc gacaagtgct
      901 attgctccgc ttggctgcac agcagaggag ccctgtggga gcaggaaaca gccaggggct
      961 tccagtggtt tgccgtgttc ctgtctgccc tgtttctggc tttctacggc tggcacgcct
     1021 ataaggccag cgtgggctgg gaggaggtgt acgtgtgctc cgtggagctg atcaaagtga
     1081 ttctggagat ctatttcgag tttaccagtc ctgcgatgct gttcctgtac ggaggcaaca
     1141 ttaccccatg gcttcgttac gccgagtggc tgctgacatg ccctgtcatt cttatccatc
     1201 tgagcaacat caccggtctg agtgaggcat ataataagcg taccatggct ctgctggtgt
     1261 cagacctggg cactatctgc atgggcgtga cagccgctct ggccactggg tgggtgaagt
     1321 ggctgtttta ctgtatcggc ctggtgtacg gcacccagac attctacaac gcaggaatca
     1381 tctacgtgga ggcgtaccac accgtgccga agggccggtg tcgccaggtg gtgactggca
     1441 tggcttggct cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg
     1501 agggcttcgg cgtcctgagc gtggctgggt ccactattgg ccacaccatc gccgacctgc
     1561 tgtccaagaa tatttgggga ctgctcgggc acttcctgcg catcaaaatc cacgagcata
     1621 tcattatgta cggcgacatt cgcagaccag tgagctccca gtttctgggc cgcaaggtgg
     1681 acgtcctggc cttcgtgaca gaggaggata aacaatctca gcctatcttg aatactaagg
     1741 agatggctcc acagagtaaa ccaccagaag agttggagat gtcatccatg ccatcaccag
     1801 tggcacccct cccagccaga accgagggcg tgattgacat gcgatccatg agttcaatag
     1861 atagcttcat tagttgtgca actgactttc ccgaagcaac cagattcgtg gcggccgcca
     1921 agagcaggat caccagcgag ggcgagtaca tccccctgga ccagatcgac atcaacgtgg
     1981 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2041 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2101 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2161 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     2221 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2281 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2341 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2401 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2461 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2521 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2581 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2641 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataag
     2701 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     2761 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2821 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2881 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2941 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3001 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3061 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3121 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     3181 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3241 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3301 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     3361 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     3421 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     3481 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     3541 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     3601 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     3661 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     3721 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     3781 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
     3841 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3901 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3961 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4021 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4081 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4141 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4201 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4261 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4321 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4381 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4441 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4501 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4561 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4621 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4681 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4741 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
