LOCUS       sequence-258261-       12753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a 
            BsmbI-flanked GFP cassette for sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE
  TITLE     CRISPR-guided DNA polymerases enable diversification of all 
            nucleotides in a tunable window.
  JOURNAL   Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 
            10.1038/s41586-018-0384-8.
  PUBMED    30069054
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 
            10.1038/s41586-018-0384-8."
COMMENT     Sequence Label: pEvolvR-enCas9-PolI3M-TBD
FEATURES             Location/Qualifiers
     source          1..12753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..78
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             96..107
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             5000..5008
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(7417..8232)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7417..8232)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8140..8159
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8224..8243
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8461..9049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8950..8969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9203..9220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9235..9374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9300..9319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9460..9482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9476..9667)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        9756..9790
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             9797..9808
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9815..10474
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKF*PAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9881..9900)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        10584..10618
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     RBS             10774..10785
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             10792..11505
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      11517..11588
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11604..11631
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        11645..11720
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             11741..11770
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             11786..11803
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11859..11876)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      12029..12075
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(12134..12753,1..4))
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     12665..12684
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 acatcattaa ttcctaattt ttgttgacac tctatcgttg atagagttat tttaccactc
       61 cctatcagtg atagagaaaa gaattcaaaa gatctaaaga ggagaaaaga tctatggaca
      121 agaagtattc tatcggactg gctatcggga ctaatagcgt cgggtgggcc gtgatcactg
      181 acgagtacaa ggtgccctct aagaagttca aggtgctcgg gaacaccgac cggcattcca
      241 tcaagaaaaa tctgatcgga gctctcctct ttgattcagg ggaaaccgct gaagcaaccc
      301 gcctcaagcg gactgctaga cggcggtaca ccaggaggaa gaaccggatt tgttaccttc
      361 aagagatatt ctccaacgaa atggcaaagg tcgacgacag cttcttccat aggctggaag
      421 aatcattcct cgtggaagag gataagaagc atgaacggca tcccatcttc ggtaatatcg
      481 tcgacgaggt ggcctatcac gagaaatacc caaccatcta ccatcttcgc aaaaagctgg
      541 tggactcaac cgacaaggca gacctccggc ttatctacct ggccctggcc cacatgatca
      601 agttcagagg ccacttcctg atcgagggcg acctcaatcc tgacaatagc gatgtggata
      661 aactgttcat ccagctggtg cagacttaca accagctctt tgaagagaac cccatcaatg
      721 caagcggagt cgatgccaag gccattctgt cagcccggct gtcaaagagc cgcagacttg
      781 agaatcttat cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg aacctgattg
      841 ctctttcact tgggctgact cccaatttca agtctaattt cgacctggca gaggatgcca
      901 agctgcaact gtccaaggac acctatgatg acgatctcga caacctcctg gcccagatcg
      961 gtgaccaata cgccgacctt ttccttgctg ctaagaatct ttctgacgcc atcctgctgt
     1021 ctgacattct ccgcgtgaac actgaaatca ccaaggcccc tctttcagct tcaatgatta
     1081 agcggtatga tgagcaccac caggacctga ccctgcttaa ggcactcgtc cggcagcagc
     1141 ttccggagaa gtacaaggaa atcttctttg accagtcaaa gaatggatac gccggctaca
     1201 tcgacggagg tgcctcccaa gaggaatttt ataagtttat caaacctatc cttgagaaga
     1261 tggacggcac cgaagagctc ctcgtgaaac tgaatcggga ggatctgctg cggaagcagc
     1321 gcactttcga caatgggagc attccccacc agatccatct tggggagctt cacgccatcc
     1381 ttcggcgcca agaggacttc tacccctttc ttaaggacaa cagggagaag attgagaaaa
     1441 ttctcacttt ccgcatcccc tactacgtgg gacccctcgc cagaggaaat agccggtttg
     1501 cttggatgac cagaaagtca gaagaaacta tcactccctg gaacttcgaa gaggtggtgg
     1561 acaagggagc cagcgctcag tcattcatcg aacggatgac taacttcgat aagaacctcc
     1621 ccaatgagaa ggtcctgccg aaacattccc tgctctacga gtactttacc gtgtacaacg
     1681 agctgaccaa ggtgaaatat gtcaccgaag ggatgaggaa gcccgcattc ctgtcaggcg
     1741 aacaaaagaa ggcaattgtg gaccttctgt tcaagaccaa tagaaaggtg accgtgaagc
     1801 agctgaagga ggactatttc aagaaaattg aatgcttcga ctctgtggag attagcgggg
     1861 tcgaagatcg gttcaacgca agcctgggta cttaccatga tctgcttaag atcatcaagg
     1921 acaaggattt tctggacaat gaggagaacg aggacatcct tgaggacatt gtcctgactc
     1981 tcactctgtt cgaggaccgg gaaatgatcg aggagaggct taagacctac gcccatctgt
     2041 tcgacgataa agtgatgaag caacttaaac ggagaagata taccggatgg ggacgcctta
     2101 gccgcaaact catcaacgga atccgggaca aacagagcgg aaagaccatt cttgatttcc
     2161 ttaagagcga cggattcgct aatcgcaact tcatgcaact tatccatgat gattccctga
     2221 cctttaagga ggacatccag aaggcccaag tgtctggaca aggtgactca ctgcacgagc
     2281 atatcgcaaa tctggctggt tcacccgcta ttaagaaggg tattctccag accgtgaaag
     2341 tcgtggacga gctggtcaag gtgatgggtc gccataaacc agagaacatt gtcatcgaga
     2401 tggccaggga aaaccagact acccagaagg gacagaagaa cagcagggag cggatgaaaa
     2461 gaattgagga agggattaag gagctcgggt cacagatcct taaagagcac ccggtggaaa
     2521 acacccagct tcagaatgag aagctctatc tgtactacct tcaaaatgga cgcgatatgt
     2581 atgtggacca agagcttgat atcaacaggc tctcagacta cgacgtggac catatcgtcc
     2641 ctcagagctt cctcgcagac gactcaattg acaataaggt gctgactcgc tcagacaaga
     2701 accggggaaa gtcagataac gtgccctcag aggaagtcgt gaaaaagatg aagaactatt
     2761 ggcgccagct tctgaacgca aagctgatca ctcagcggaa gttcgacaat ctcactaagg
     2821 ctgagagggg cggactgagc gaactggaca aagcaggatt cattaaacgg caacttgtgg
     2881 agactcggca gattactaaa catgtcgccc aaatccttga ctcacgcatg aataccaagt
     2941 acgacgaaaa cgacaaactt atccgcgagg tgaaggtgat taccctgaag tccaagctgg
     3001 tcagcgattt cagaaaggac tttcaattct acaaagtgcg ggagatcaat aactatcatc
     3061 atgctcatga cgcatatctg aatgccgtgg tgggaaccgc cctgatcaag aagtacccag
     3121 cactggaaag cgagttcgtg tacggagact acaaggtcta cgacgtgcgc aagatgattg
     3181 ccaaatctga gcaggagatc ggaaaggcca ccgcaaagta cttcttctac agcaacatca
     3241 tgaatttctt caagaccgaa atcacccttg caaacggtga gatccggaag gcgccgctca
     3301 tcgagactaa tggggagact ggcgaaatcg tgtgggacaa gggcagagat ttcgctaccg
     3361 tgcgcaaagt gctttctatg cctcaagtga acatcgtgaa gaaaaccgag gtgcaaaccg
     3421 gaggcttttc taaggaatca atcctcccca agcgcaactc cgacaagctc attgcaagga
     3481 agaaggattg ggaccctaag aagtacggcg gattcgattc accaactgtg gcttattctg
     3541 tcctggtcgt ggctaaggtg gaaaaaggaa agtctaagaa gctcaagagc gtgaaggaac
     3601 tgctgggtat caccattatg gagcgcagct ccttcgagaa gaacccaatt gactttctcg
     3661 aagccaaagg ttacaaggaa gtcaagaagg accttatcat caagctccca aagtatagcc
     3721 tgttcgaact ggagaatggg cggaagcgga tgctcgcctc cgctggcgaa cttcagaagg
     3781 gtaatgagct ggctctcccc tccaagtacg tgaatttcct ctaccttgca agccattacg
     3841 agaagctgaa ggggagcccc gaggacaacg agcaaaagca actgtttgtg gagcagcata
     3901 agcattatct ggacgagatc attgagcaga tttccgagtt ttctaaacgc gtcattctcg
     3961 ctgatgccaa cctcgataaa gtccttagcg catacaataa gcacagagac aaaccaattc
     4021 gggagcaggc tgagaatatc atccacctgt tcaccctcac caatcttggt gcccctgccg
     4081 cattcaagta cttcgacacc accatcgacc ggaaacgcta tacctccacc aaagaagtgc
     4141 tggacgccac cctcatccac cagagcatca ccggacttta cgaaactcgg attgacctct
     4201 cacagctcgg aggggatggt tctagtgaaa ccccgggaac aagtgagtcg gccacccctg
     4261 aaggtggatc agggggtagc ggatccgttc agatcccgca gaacccgctg attctggttg
     4321 acggatctag ttacctgtac cgtgcttacc atgctttccc gcctttgacc aattctgctg
     4381 gtgaacctac gggagctatg tacggagttc tgaatatgtt gcgttcttta attatgcagt
     4441 acaagcctac ccacgctgct gttgttttcg atgctaaagg taagacgttc cgcgacgagt
     4501 tattcgagca ctataagtct caccgtcctc cgatgcctga tgacttacgc gctcagattg
     4561 agccgctgca tgctatggtg aaggctatgg gtttacctct tttggctgtc agcggtgttg
     4621 aggctgatga tgtcattggc accttagctc gtgaggctga gaaggctggt cgccctgttt
     4681 tgatttctac cggtgacaag gacatggctc aattggttac cccgaacatc accctgatca
     4741 acaccatgac caacacgatt ctgggtcctg aggaagttgt taacaaatat ggtgttcctc
     4801 cggagttgat tattgacttt cttgctctta tgggcgattc ttcagacaat atcccgggtg
     4861 ttccaggtgt tggagagaag actgctcaag ctctgcttca gggtctgggt ggtttggaca
     4921 ccctttacgc tgaaccggag aagatcgccg gtctgtcttt tcgcggtgct aagaccatgg
     4981 ctgctaaact ggaacagaat aaggaggtcg catacctgtc ttatcaattg gctaccatca
     5041 agacggatgt ggagttagaa cttacgtgcg agcagcttga ggttcaacag cctgctgctg
     5101 aggaactgct gggtcttttt aagaaatacg aatttaagcg ttggaccgcc gacgttgagg
     5161 ctggtaagtg gctgcaagct aagggtgcta agccggctgc taaaccgcaa gaaacgagtg
     5221 tcgctgatga ggctccggag gttaccgcta ccgttatctc ttacgataat tatgttacga
     5281 ttctggacga ggaaacctta aaggcttgga tcgctaaatt agagaaggct cctgttttcg
     5341 ctttcgacac ggaaacggat tctctggaca atattagtgc gaatcttgtt ggtctgagtt
     5401 tcgcaattga accgggtgtt gctgcttaca tccctgtggc acacgactac ctggacgctc
     5461 cggaccagat ttcacgtgaa cgcgctctgg aactgctgaa gcctttatta gaggacgaga
     5521 aagctttgaa agttggtcag aatttgaagt atgctcgtgg aatcttagct aattatggta
     5581 tcgagttgcg cggtatcgct ttcgacacga tgttggaatc ttatatcctg aactctgtcg
     5641 ctggtcgcca tgacatggac tctctggctg agcgctggct gaaacataag acgattacct
     5701 tcgaggaaat cgcaggaaag ggtaagaacc agctcacgtt caatcaaatc gctctggagg
     5761 aagctggtcg ctatgctgct gaggacgctg acgttactct gcaactgcac ttgaagatgt
     5821 ggcctgactt gcagaagcat aagggtccac tgaatgtttt tgaaaacatt gagatgcctt
     5881 tggttccagt tctgtctcgt atcgagcgca atggcgttaa aattgaccca aaggttttac
     5941 ataaccactc agaggaactg acgctgcgct tagccgaatt ggagaaaaag gctaccgaaa
     6001 cgttcggctc gtggtatcag cctaaaggtg gcactgagat gttctgccat ccgcgaacag
     6061 gtaagccact acctaaatac cctcgcatta agacacctaa agttggtggt atctttaaga
     6121 agcctaagaa caaggcacag cgagaaggcc gtgagccttg cgaacttgat acccgcgagt
     6181 acgttgctgg tgctccttac accccagttg aacatgttgt gttcaatctg tcatctacga
     6241 aacaactgca gactatcctg ttcgagaagc aaggtatcaa gccattaaaa aagacccctg
     6301 gcggtgctcc gtctacctct gaggaagttt tggaggagtt agctttggat taccctctgc
     6361 cgaaggttat cttggaatac cgcggtttgg ctaaattgaa gtctacttat acggataaac
     6421 ttcctttgat gattaatcca aagacgggtc gcgttcacac gtcgtaccat caagctgtta
     6481 ccgctaccgg tcgcctgtct tctacggatc cgaatttaca gaatattcct gtgcgcaatg
     6541 aggagggccg ccgcattcgt caagctttta tcgctccgga agactacgtt atcgtttctg
     6601 ctgattattc tcaaaatgaa ttacgtatca tggctcacct gtctcgcgat aagggtctgt
     6661 tgacggcctt tgctgagggt aaggacattc atcgtgctac cgctgctgag gttttcggcc
     6721 tgccgttgga aacggttacg tctgaacagc gtcgctctgc taagcgtatt aatttcggct
     6781 taatctacgg tatgtctgcg tttggcttag ctcgtcagct gaatatcccg cgcaaggaag
     6841 ctcaaaaata tatggatctg tattttgagc gttacccggg tgttttggaa tacatggagc
     6901 gtacgcgcgc gcaagctaag gaacaaggtt atgtggaaac cttggatggt cgtcgcttgt
     6961 acttgcctga cattaagtct tctaacggcg cccgccgcgc tgctgccgag cgcgctgcta
     7021 tcaatgctcc gatgcaaggt actgctgctg atattattaa gcgtgctatg atcgctgtgg
     7081 acgcttggct gcaagctgaa cagcctcgcg ttcgcatgat tatgcaagtt catgacgagt
     7141 tggttttcga ggtgcataag gacgacgtgg acgctgttgc taaacaaatc caccagttga
     7201 tggagaattg cacgcgctta gacgttccgc tgctggttga agttggttct ggtgaaaact
     7261 gggaccaggc tcactaataa tggcctcggt accaaagacg aacaataaga cgctgaaaag
     7321 cgtctttttt cgttttggtc cgctgagcag ttacagagat gttacgaacc actagtgcac
     7381 tgcagtacag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
     7441 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     7501 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     7561 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     7621 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     7681 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     7741 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg
     7801 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     7861 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     7921 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     7981 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     8041 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     8101 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     8161 atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg
     8221 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     8281 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     8341 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag gagttcatga ccaaaatccc
     8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8581 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     9241 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     9301 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     9361 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     9421 tgctcccggc atccgcttac agacaagctg tgaccttctc cgggagctgc atgtgtcaga
     9481 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     9541 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     9601 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     9661 tggtcactga tgcctccgtg taagggggat ttctgttcat ccctgaattc gcatctagac
     9721 tgaactggcc gataattgca gacgaacgga tttactttac agctagctca gtcctaggta
     9781 ttatgctagc gctagcaaag aggagaaaag atctatggag aaaaaaatca ctggatatac
     9841 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     9901 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     9961 gaaaaataag cacaagtttt aaccggcctt tattcacatt cttgcccgcc tgatgaatgc
    10021 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
    10081 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
    10141 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
    10201 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt cagccaatcc
    10261 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
    10321 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
    10381 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
    10441 acagtactgc gatgagtggc agggcggggc gtaaggatcc taactcgagc tcggtaccaa
    10501 attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc gctgccaaac
    10561 cagatgtcaa cacagctaca acgtttatag ctagctcagc ccttggtaca atgctagctg
    10621 agacggaaag tgaaacgtga tttcatgcgt cattttgaac attttgtaaa tcttatttaa
    10681 taatgtgtgc ggcaattcac atttaattta tgaatgtttt cttaacatcg cggcaactca
    10741 agaaacggca ggttcggatc ttagctacta gagaaagagg agaaatacta gatgcgtaaa
    10801 ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac
    10861 ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg
    10921 ctgaagttca tctgtactac tggtaaactg ccggttcctt ggccgactct ggtaacgacg
    10981 ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc
    11041 ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt taaggatgac
    11101 ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt
    11161 gagctgaaag gcattgactt taaagaggac ggcaatatcc tgggccataa gctggaatac
    11221 aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg cattaaagcg
    11281 aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga tcactaccag
    11341 caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta tctgagcacg
    11401 caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct gctggagttc
    11461 gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgacc aggcatcaaa
    11521 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
    11581 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacgt
    11641 ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
    11701 aagtggcacc gagtcggtgc tttttttgaa gcttgggccc gaacaaaaac tcatctcaga
    11761 agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt aaacggactc
    11821 cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
    11881 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgaggt ggtcccacct
    11941 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggactccc
    12001 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
    12061 ggcctttcgt tttatctgtt gtttgtcggt gaacgacagg gctggatgca cacactggct
    12121 taagatgaca acgttaagac ccactttcac atttaagttg tttttctaat ccgcatatga
    12181 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
    12241 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
    12301 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
    12361 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
    12421 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
    12481 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
    12541 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
    12601 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
    12661 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
    12721 cgctgttaat cactttactt ttatctaatc tag
//
