LOCUS       sequence-258267-       11286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  frameshift reporter for barcode retrieval.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Feldman D, Tsai F, Garrity AJ, O'Rourke R, Brenan L, Ho P, Gonzalez 
            E, Konermann S, Johannessen CM, Beroukhim R, Bandopadhayay P, 
            Blainey PC
  TITLE     CloneSifter: enrichment of rare clones from heterogeneous cell 
            populations.
  JOURNAL   BMC Biol. 2020 Nov 24;18(1):177. doi: 10.1186/s12915-020-00911-3.
  PUBMED    33234154
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2020-11-24"; volume: "18(1):177. doi"; pages: " 
            10.1186/s12915-020-00911-3"
COMMENT     Sequence Label: pLenti_TMv2
FEATURES             Location/Qualifiers
     source          1..11286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3945..3998
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4248..4257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4721..4777
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4778..5170
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5186..5239
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5978..6566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6031..6051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6449..6460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6569..6585)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6570..6586)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7091..7271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7297..7314)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7303..7527
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7573..8001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7660..7679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7870..7891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8010..8030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8015..8344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8195..8330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8257..8276
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8392..8439
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8458..8832
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8962..9083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8999..9018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9053..9072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9132..9148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9132..9148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9145..9167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9156..9172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9180..9186,9187..9204,9205..9210))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9225..9246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9363..9380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9534..10122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9614..9633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10293..11084,11085..11153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10916..10935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11154..11258)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgaaccgg ttaatagtga gtagtagtaa gtgataatag
     3901 aaatcgagac gctcagactc agacgtctct gtacaggcag tggagagggc agaggaagtc
     3961 tgctcacctg cggcgacgtc gaggagaatc ctggcccagt cagcaagggc gaggaggaca
     4021 acgcctctct cccagcgaca cacgagttac acatctttgg ctccatcaac ggtgtggact
     4081 tcgacctcgt gggtcagggc accggcaatc caaacgacgg ttacgaggag ctcaacctca
     4141 agtccaccaa gggcgacctc cagttctccc cctggattct ggtccctcat atcgggtacg
     4201 gcttccatca gtacctgccc tacccagacg ggatgtcgcc tttccaggcc gccatggtgg
     4261 acggctccgg ataccaagtc catcgcacaa tgcagttcga agacggtgcc tcccttactg
     4321 tcaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag gtcaagggga
     4381 ctggtttccc tgccgacggt cctgtcctca ccaactcgct caccgctgcg gactggtgca
     4441 ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag taccttcaag tggagttaca
     4501 ccactggaaa cggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc
     4561 caatggcggc caactatctc aagaaccagc cgatgtacgt gttccgcaag acggagctca
     4621 agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gacgtcatgg
     4681 gcatggacga gctctacaag taataataac cggatccggc gcaacaaact tctctctgct
     4741 caaacaagcc ggcgacgtcg aagagaatcc tgggccggcc aagcctttgt ctcaagaaga
     4801 atccaccctc atcgaaagag caacggctac aatcaacagc atccccatct ccgaagacta
     4861 cagcgtcgcc agcgcagctc tctcaagcga cggccgcatc ttcactggtg tcaacgtata
     4921 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     4981 agctggcaac ctcacttgta tcgtcgcgat cggaaacgag aacaggggca tcctcagccc
     5041 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatcgtcaa
     5101 ggacagcgac ggacagccga cggcagttgg gattcgcgaa ttgctgccct ctggttacgt
     5161 gtgggagggc tgtacgggca gtggggaggg cagaggaagt ctgctaacct gcggtgacgt
     5221 cgaggagaat cctggcccag tgtccaaggg cgaggaagat aacatggcca tcatcaagga
     5281 gttcatgagg tttaaggtcc acatggaggg ttcagtcaat ggccacgagt tcgagattga
     5341 aggcgagggc gagggccgcc cctacgaagg gacacagacg gcgaaattga aggtgaccaa
     5401 aggcgggcca ttgcccttcg catgggacat cttgtcccct cagtttatgt atggcagcaa
     5461 ggcctatgtt aagcaccccg ctgatatccc ggactacttg aagctgtcct ttccagaggg
     5521 gtttaaatgg gagcgcgtta tgaatttcga agacggagga gtggttacgg tgacgcagga
     5581 ctcatccctg caggacggag aatttatata taaggttaag ttgagaggca caaacttccc
     5641 aagcgacggc cctgtgatgc agaagaaaac aatggggtgg gaagcttcca gcgagcgcat
     5701 gtaccccgaa gatggcgccc tcaagggcga gataaagcaa aggctgaaac ttaaggacgg
     5761 cggtcattac gacgcggagg tcaagacaac ttacaaggct aaaaaacccg ttcagttgcc
     5821 tggggcttac aatgttaata tcaaacttga catcacaagc cacaatgaag actatacgat
     5881 cgtggagcag tatgaacgag cggaaggcag gcactcaacg ggggggatgg acgagcttta
     5941 caagtaataa taagaattcg atatcaagct tatcggtaat caacctctgg attacaaaat
     6001 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6061 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6121 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6181 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6241 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6301 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6361 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6421 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6481 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6541 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     6601 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6661 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6721 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6781 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6841 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     6901 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     6961 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7021 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     7081 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7261 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7321 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7381 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7441 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7501 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7561 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7981 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8161 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8221 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8281 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8341 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8401 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8461 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     8521 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     8581 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     8641 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     8701 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     8761 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     8821 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8881 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8941 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9061 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9121 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9181 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9241 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9301 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9661 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9721 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9781 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9841 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9901 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9961 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10021 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10081 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10141 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10201 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10261 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10321 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10381 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10441 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10501 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10561 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10621 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10681 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10741 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10801 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10861 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10921 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10981 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11041 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11101 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11161 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11221 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11281 cctgac
//
