LOCUS       sequence-258271-       11727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  frameshift reporter for barcode retrieval (with Zeo selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Feldman D, Tsai F, Garrity AJ, O'Rourke R, Brenan L, Ho P, Gonzalez 
            E, Konermann S, Johannessen CM, Beroukhim R, Bandopadhayay P, 
            Blainey PC
  TITLE     CloneSifter: enrichment of rare clones from heterogeneous cell 
            populations.
  JOURNAL   BMC Biol. 2020 Nov 24;18(1):177. doi: 10.1186/s12915-020-00911-3.
  PUBMED    33234154
REFERENCE   2  (bases 1 to 11727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2020-11-24"; volume: "18(1):177. doi"; pages: " 
            10.1186/s12915-020-00911-3"
COMMENT     Sequence Label: pLenti_TMv2_Zeo
FEATURES             Location/Qualifiers
     source          1..11727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4386..4439
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5162..5218
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5219..5611
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5627..5680
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6419..7007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6472..6492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6890..6901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7010..7026)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7011..7027)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7532..7712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7738..7755)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7744..7968
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8014..8442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8101..8120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8311..8332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8451..8471)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8456..8785
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8636..8771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8698..8717
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8833..8880
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8899..9273
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9403..9524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9440..9459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9494..9513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9573..9589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9573..9589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9586..9608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9597..9613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9621..9627,9628..9645,9646..9651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9666..9687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9804..9821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9975..10563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10055..10074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10734..11525,11526..11594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11357..11376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11595..11699)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgaaccgg ttaatagtga gtagtagtaa gtgataatag
     3901 aaatcgagac gctcagactc agacgtctct gtacaggctc cggcgcaacc aacttctctc
     3961 tcctcaaaca agctggagaa gtggaggaga atcccgggcc ggcaaagctc acgagcgccg
     4021 ttcctgtcct taccgcccgc gacgtggcag gtgctgtcga gttttggaca gatcgcctcg
     4081 gattctctcg cgattttgtc gaggacgact tcgcaggagt ggtcagggac gacgtcaccc
     4141 tgtttatctc tgcagttcaa gaccaagttg tgccggacaa tactctcgcg tgggtgtggg
     4201 ttcggggcct cgacgagctg tacgccgagt ggagcgaagt tgtctcaacc aacttcaggg
     4261 acgcctccgg tcccgctctc acagaaatcg gcgagcagcc ttggggacga gagttcgctc
     4321 tcagggaccc cgcaggcaac tgtgttcatt tcgtcgcaga agaacaggac ctcgagggca
     4381 gtggagaggg cagaggaagt ctgctcacct gcggcgacgt cgaggagaat cctggcccag
     4441 tcagcaaggg cgaggaggac aacgcctctc tcccagcgac acacgagtta cacatctttg
     4501 gctccatcaa cggtgtggac ttcgacctcg tgggtcaggg caccggcaat ccaaacgacg
     4561 gttacgagga gctcaacctc aagtccacca agggcgacct ccagttctcc ccctggattc
     4621 tggtccctca tatcgggtac ggcttccatc agtacctgcc ctacccagac gggatgtcgc
     4681 ctttccaggc cgccatggtg gacggctccg gataccaagt ccatcgcaca atgcagttcg
     4741 aagacggtgc ctcccttact gtcaactacc gctacaccta cgagggaagc cacatcaaag
     4801 gagaggccca ggtcaagggg actggtttcc ctgccgacgg tcctgtcctc accaactcgc
     4861 tcaccgctgc ggactggtgc aggtcgaaga agacttaccc caacgacaaa accatcatca
     4921 gtaccttcaa gtggagttac accactggaa acggcaagcg ctaccggagc actgcgcgga
     4981 ccacctacac ctttgccaag ccaatggcgg ccaactatct caagaaccag ccgatgtacg
     5041 tgttccgcaa gacggagctc aagcactcca agaccgagct caacttcaag gagtggcaaa
     5101 aggcctttac cgacgtcatg ggcatggacg agctctacaa gtaataataa ccggatccgg
     5161 cgcaacaaac ttctctctgc tcaaacaagc cggcgacgtc gaagagaatc ctgggccggc
     5221 caagcctttg tctcaagaag aatccaccct catcgaaaga gcaacggcta caatcaacag
     5281 catccccatc tccgaagact acagcgtcgc cagcgcagct ctctcaagcg acggccgcat
     5341 cttcactggt gtcaacgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     5401 gggcactgct gctgctgcgg cagctggcaa cctcacttgt atcgtcgcga tcggaaacga
     5461 gaacaggggc atcctcagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     5521 tgggatcaaa gccatcgtca aggacagcga cggacagccg acggcagttg ggattcgcga
     5581 attgctgccc tctggttacg tgtgggaggg ctgtacgggc agtggggagg gcagaggaag
     5641 tctgctaacc tgcggtgacg tcgaggagaa tcctggccca gtgtccaagg gcgaggaaga
     5701 taacatggcc atcatcaagg agttcatgag gtttaaggtc cacatggagg gttcagtcaa
     5761 tggccacgag ttcgagattg aaggcgaggg cgagggccgc ccctacgaag ggacacagac
     5821 ggcgaaattg aaggtgacca aaggcgggcc attgcccttc gcatgggaca tcttgtcccc
     5881 tcagtttatg tatggcagca aggcctatgt taagcacccc gctgatatcc cggactactt
     5941 gaagctgtcc tttccagagg ggtttaaatg ggagcgcgtt atgaatttcg aagacggagg
     6001 agtggttacg gtgacgcagg actcatccct gcaggacgga gaatttatat ataaggttaa
     6061 gttgagaggc acaaacttcc caagcgacgg ccctgtgatg cagaagaaaa caatggggtg
     6121 ggaagcttcc agcgagcgca tgtaccccga agatggcgcc ctcaagggcg agataaagca
     6181 aaggctgaaa cttaaggacg gcggtcatta cgacgcggag gtcaagacaa cttacaaggc
     6241 taaaaaaccc gttcagttgc ctggggctta caatgttaat atcaaacttg acatcacaag
     6301 ccacaatgaa gactatacga tcgtggagca gtatgaacga gcggaaggca ggcactcaac
     6361 gggggggatg gacgagcttt acaagtaata ataagaattc gatatcaagc ttatcggtaa
     6421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6781 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6961 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7021 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     7081 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7141 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7201 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7261 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     7321 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     7381 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     7441 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     7501 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     7561 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7681 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7741 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7801 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7861 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7921 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7981 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     8041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8461 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8521 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8581 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8701 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8761 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8821 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     8881 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     8941 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     9001 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     9061 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     9121 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     9181 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     9241 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     9301 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9361 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9541 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9721 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10381 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11701 acatttcccc gaaaagtgcc acctgac
//
