LOCUS       sequence-258281-        9326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of both AsCas12a and CRISPR arrays on single 
            transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
  TITLE     Multiplexed genome engineering by Cas12a and CRISPR arrays encoded 
            on single transcripts.
  JOURNAL   Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6.
  PUBMED    31406383
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6."
COMMENT     Sequence Label: pCI118-SiT-Cas12a-[Ind]
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    273..291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    309..327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    344..362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    380..398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        410..448
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     493..512
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        493..511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             559..4479
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4199..4208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4480..4527
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4534..4614
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4917..4934)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4923..5147
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5193..5621
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5280..5299)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5490..5511
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5630..5650)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5635..5964
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5815..5950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5877..5896
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6031..6825
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6085..6104)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6695..6714
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6999..7120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7036..7055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7090..7109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7169..7185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7169..7185)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7182..7204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7193..7209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7217..7223,7224..7241,7242..7247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7262..7283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7400..7417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7571..8159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7651..7670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8330..9121,9122..9190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8953..8972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9191..9295)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttcgcgttg acattgatta
      181 ttgactagtg gcatggacga gctgtacaag tagagcgctg ctagcctcga gtttactccc
      241 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      301 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
      361 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
      421 acggtgggag gcctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      481 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
      541 aagcttggta ccgccaccat gacacagttc gagggcttta ccaacctgta tcaggtgagc
      601 aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat ccaggagcag
      661 ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa gcccatcatc
      721 gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct ggattgggag
      781 aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac aaggaacgcc
      841 ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat cggccggaca
      901 gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg cctgttcaag
      961 gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac aaccgagcac
     1021 gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg cttttatgag
     1081 aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca ccgcatcgtg
     1141 caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct gatcaccgcc
     1201 gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat cttcgtgagc
     1261 acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac acagacccag
     1321 atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac cgagaagatc
     1381 aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac agcccacatc
     1441 atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc cgataggaac
     1501 accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca gtccttctgc
     1561 aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc cctgtttaac
     1621 gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct ggagacaatc
     1681 agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga gcggagaatc
     1741 tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg cagcctgaag
     1801 cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct gagcgaggcc
     1861 ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga tcagccactg
     1921 cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct ggacagcctg
     1981 ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga ggtggacccc
     2041 gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct gagcttctac
     2101 aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt caagctgaac
     2161 tttcagatgc ctacactggc ctctggctgg gacgtgaata aggagaagaa caatggcgcc
     2221 atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca gaagggcagg
     2281 tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga taagatgtac
     2341 tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca gctgaaggcc
     2401 gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa tttcatcgag
     2461 cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga gccaaagaag
     2521 tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga ggccctgtgc
     2581 aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac ctctatcgat
     2641 ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta tgccgagctg
     2701 aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat catggatgcc
     2761 gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc caagggccac
     2821 cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc agagaacctg
     2881 gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc taagtccagg
     2941 atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct gaaggatcag
     3001 aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa tcacagactg
     3061 tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac caaggaggtg
     3121 tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt ccacgtgcct
     3181 atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag ggtgaatgcc
     3241 tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga gagaaacctg
     3301 atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag cctgaacacc
     3361 atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag ggtggcagca
     3421 aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta tctgagccag
     3481 gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt gctggagaac
     3541 ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt gtaccagcag
     3601 ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta tccagcagag
     3661 aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc ctttgccaag
     3721 atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc taagatcgat
     3781 cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca cgagagccgc
     3841 aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg cgacttcatc
     3901 ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg ctttatgcct
     3961 gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg cacccctttc
     4021 atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg cagataccgg
     4081 gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat cgtgttcagg
     4141 gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc catcgacacc
     4201 atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc cacaggcgag
     4261 gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc ccggtttcag
     4321 aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc cctgaagggc
     4381 cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa cggcatctcc
     4441 aatcaggact ggctggccta catccaggag ctgcgcaaca aaaggccggc ggccacgaaa
     4501 aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc agattacgct
     4561 tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta tgcctaagaa
     4621 ttcgattcgt cagtagggtt gtaaaggttt ttcttttcct gagaaaacaa ccttttgttt
     4681 tctcaggttt tgctttttgg cctttcccta gctttaaaaa aaaaaaagca aaactcaccg
     4741 aggcagttcc ataggatggc aagatcctgg tattggtctg cgaaatttct actcttgtag
     4801 atggagacgc aaattctaat ccagcttcaa cagctatttc acaagctttc atgatttctc
     4861 gtctctaatt tctactcttg tagattctag agggcccgtt taaacccgct gatcagcctc
     4921 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4981 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5041 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5101 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5161 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5221 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5281 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5341 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5401 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5461 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5521 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5581 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5641 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5701 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5761 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5821 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5881 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5941 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6001 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6061 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6121 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6181 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6241 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6301 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6361 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6421 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6481 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6541 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6601 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6661 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6721 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6781 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6841 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6901 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6961 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7141 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7201 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7261 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7321 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7381 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7501 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7561 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7741 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7801 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7861 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7921 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7981 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8041 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8101 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8161 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8221 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8281 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8341 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8401 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8461 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8521 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8581 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8641 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8701 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8761 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8821 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8881 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8941 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9001 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9061 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9301 ttccccgaaa agtgccacct gacgtc
//
