LOCUS       sequence-258285-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express both AsCas12a fused to [Activ] activator domain and CRISPR 
            arrays on a single transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
  TITLE     Multiplexed genome engineering by Cas12a and CRISPR arrays encoded 
            on single transcripts.
  JOURNAL   Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6.
  PUBMED    31406383
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6."
COMMENT     Sequence Label: pCE059-SiT-Cas12a-[Activ]
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        233..1411
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          464..1402
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1359..1379
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1446..5366
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5086..5095
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5367..5414
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5733..6104
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             6111..6191
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     6388..6404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6388..6404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    6465..7053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6518..6538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6936..6947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   7140..7280
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7140..7269
                     /label=AAV2 ITR
     rep_origin      7355..7810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7442..7461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7652..7673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7827..7846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7946..7968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8006..8024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8092..8196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8197..8265,8266..9057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8415..8434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9228..9816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9717..9736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaatt aatgcaaaga tggataaagt tttaaacaga
      181 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      241 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      301 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      361 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      421 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      481 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      541 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      601 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      661 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      721 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      781 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      841 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
      901 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
      961 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1021 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1081 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1141 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1201 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1261 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1321 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1381 aagttttttt cttccatttc aggtgtcgtg agctagcgtt taaacttaag cttggtaccg
     1441 ccaccatgac acagttcgag ggctttacca acctgtatca ggtgagcaag acactgcggt
     1501 ttgagctgat cccacagggc aagaccctga agcacatcca ggagcagggc ttcatcgagg
     1561 aggacaaggc ccgcaatgat cactacaagg agctgaagcc catcatcgat cggatctaca
     1621 agacctatgc cgaccagtgc ctgcagctgg tgcagctgga ttgggagaac ctgagcgccg
     1681 ccatcgactc ctatagaaag gagaaaaccg aggagacaag gaacgccctg atcgaggagc
     1741 aggccacata tcgcaatgcc atccacgact acttcatcgg ccggacagac aacctgaccg
     1801 atgccatcaa taagagacac gccgagatct acaagggcct gttcaaggcc gagctgttta
     1861 atggcaaggt gctgaagcag ctgggcaccg tgaccacaac cgagcacgag aacgccctgc
     1921 tgcggagctt cgacaagttt acaacctact tctccggctt ttatgagaac aggaagaacg
     1981 tgttcagcgc cgaggatatc agcacagcca tcccacaccg catcgtgcag gacaacttcc
     2041 ccaagtttaa ggagaattgt cacatcttca cacgcctgat caccgccgtg cccagcctgc
     2101 gggagcactt tgagaacgtg aagaaggcca tcggcatctt cgtgagcacc tccatcgagg
     2161 aggtgttttc cttccctttt tataaccagc tgctgacaca gacccagatc gacctgtata
     2221 accagctgct gggaggaatc tctcgggagg caggcaccga gaagatcaag ggcctgaacg
     2281 aggtgctgaa tctggccatc cagaagaatg atgagacagc ccacatcatc gcctccctgc
     2341 cacacagatt catccccctg tttaagcaga tcctgtccga taggaacacc ctgtctttca
     2401 tcctggagga gtttaagagc gacgaggaag tgatccagtc cttctgcaag tacaagacac
     2461 tgctgagaaa cgagaacgtg ctggagacag ccgaggccct gtttaacgag ctgaacagca
     2521 tcgacctgac acacatcttc atcagccaca agaagctgga gacaatcagc agcgccctgt
     2581 gcgaccactg ggatacactg aggaatgccc tgtatgagcg gagaatctcc gagctgacag
     2641 gcaagatcac caagtctgcc aaggagaagg tgcagcgcag cctgaagcac gaggatatca
     2701 acctgcagga gatcatctct gccgcaggca aggagctgag cgaggccttc aagcagaaaa
     2761 ccagcgagat cctgtcccac gcacacgccg ccctggatca gccactgcct acaaccctga
     2821 agaagcagga ggagaaggag atcctgaagt ctcagctgga cagcctgctg ggcctgtacc
     2881 acctgctgga ctggtttgcc gtggatgagt ccaacgaggt ggaccccgag ttctctgccc
     2941 ggctgaccgg catcaagctg gagatggagc cttctctgag cttctacaac aaggccagaa
     3001 attatgccac caagaagccc tactccgtgg agaagttcaa gctgaacttt cagatgccta
     3061 cactggcctc tggctgggac gtgaataagg agaagaacaa tggcgccatc ctgtttgtga
     3121 agaacggcct gtactatctg ggcatcatgc caaagcagaa gggcaggtat aaggccctga
     3181 gcttcgagcc cacagagaaa accagcgagg gctttgataa gatgtactat gactacttcc
     3241 ctgatgccgc caagatgatc ccaaagtgca gcacccagct gaaggccgtg acagcccact
     3301 ttcagaccca cacaaccccc atcctgctgt ccaacaattt catcgagcct ctggagatca
     3361 caaaggagat ctacgacctg aacaatcctg agaaggagcc aaagaagttt cagacagcct
     3421 acgccaagaa aaccggcgac cagaagggct acagagaggc cctgtgcaag tggatcgact
     3481 tcacaaggga ttttctgtcc aagtatacca agacaacctc tatcgatctg tctagcctgc
     3541 ggccatcctc tcagtataag gacctgggcg agtactatgc cgagctgaat cccctgctgt
     3601 accacatcag cttccagaga atcgccgaga aggagatcat ggatgccgtg gagacaggca
     3661 agctgtacct gttccagatc tataacaagg actttgccaa gggccaccac ggcaagccta
     3721 atctgcacac actgtattgg accggcctgt tttctccaga gaacctggcc aagacaagca
     3781 tcaagctgaa tggccaggcc gagctgttct accgccctaa gtccaggatg aagaggatgg
     3841 cacaccggct gggagagaag atgctgaaca agaagctgaa ggatcagaaa accccaatcc
     3901 ccgacaccct gtaccaggag ctgtacgact atgtgaatca cagactgtcc cacgacctgt
     3961 ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa ggaggtgtct cacgagatca
     4021 tcaaggatag gcgctttacc agcgacaagt tctttttcca cgtgcctatc acactgaact
     4081 atcaggccgc caattcccca tctaagttca accagagggt gaatgcctac ctgaaggagc
     4141 accccgagac acctatcatc ggcatcgatc ggggcgagag aaacctgatc tatatcacag
     4201 tgatcgactc caccggcaag atcctggagc agcggagcct gaacaccatc cagcagtttg
     4261 attaccagaa gaagctggac aacagggaga aggagagggt ggcagcaagg caggcctggt
     4321 ctgtggtggg cacaatcaag gatctgaagc agggctatct gagccaggtc atccacgaga
     4381 tcgtggacct gatgatccac taccaggccg tggtggtgct ggagaacctg aatttcggct
     4441 ttaagagcaa gaggaccggc atcgccgaga aggccgtgta ccagcagttc gagaagatgc
     4501 tgatcgataa gctgaattgc ctggtgctga aggactatcc agcagagaaa gtgggaggcg
     4561 tgctgaaccc ataccagctg acagaccagt tcacctcctt tgccaagatg ggcacccagt
     4621 ctggcttcct gttttacgtg cctgccccat atacatctaa gatcgatccc ctgaccggct
     4681 tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga gagccgcaag cacttcctgg
     4741 agggcttcga ctttctgcac tacgacgtga aaaccggcga cttcatcctg cactttaaga
     4801 tgaacagaaa tctgtccttc cagaggggcc tgcccggctt tatgcctgca tgggatatcg
     4861 tgttcgagaa gaacgagaca cagtttgacg ccaagggcac ccctttcatc gccggcaaga
     4921 gaatcgtgcc agtgatcgag aatcacagat tcaccggcag ataccgggac ctgtatcctg
     4981 ccaacgagct gatcgccctg ctggaggaga agggcatcgt gttcagggat ggctccaaca
     5041 tcctgccaaa gctgctggag aatgacgatt ctcacgccat cgacaccatg gtggccctga
     5101 tccgcagcgt gctgcagatg cggaactcca atgccgccac aggcgaggac tatatcaaca
     5161 gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg gtttcagaac ccagagtggc
     5221 ccatggacgc cgatgccaat ggcgcctacc acatcgccct gaagggccag ctgctgctga
     5281 atcacctgaa ggagagcaag gatctgaagc tgcagaacgg catctccaat caggactggc
     5341 tggcctacat ccaggagctg cgcaacaaaa ggccggcggc cacgaaaaag gccggccagg
     5401 caaaaaagaa aaagggatcc ggtgggggtg ggtccagcgg gttgcctaat gggttggatg
     5461 gtgacgagga ttttagcgat atcgccgata tggacttttc agctctcctc agccagataa
     5521 gctctggaag tagtgggctt ccaaatgggc ttgacggtga cgaagatttc tctgatatag
     5581 cggacatgga cttctcagct ctgctctccc agatttcttc agggtccagc ggcttgccca
     5641 acggcctcga tggcgacgag gatttttctg acatagcaga catggacttt tccgcactcc
     5701 tcagtcagat ttcttctgga ggcggtggaa gcggcttcag cgtggacacc agtgccctgc
     5761 tggacctgtt cagcccctcg gtgaccgtgc ccgacatgag cctgcctgac cttgacagca
     5821 gcctggccag tatccaagag ctcctgtctc cccaggagcc ccccaggcct cccgaggcag
     5881 agaacagcag cccggattca gggaagcagc tggtgcacta cacagcgcag ccgctgttcc
     5941 tgctggaccc cggctccgtg gacaccggga gcaacgacct gccggtgctg tttgagctgg
     6001 gagagggctc ctacttctcc gaaggggacg gcttcgccga ggaccccacc atctccctgc
     6061 tgacaggctc ggagcctccc aaagccaagg accccactgt ctccggatcc tacccatacg
     6121 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     6181 ccgactatgc ctaagaattc gattcgtcag tagggttgta aaggtttttc ttttcctgag
     6241 aaaacaacct tttgttttct caggttttgc tttttggcct ttccctagct ttaaaaaaaa
     6301 aaaagcaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat tggtctgcga
     6361 aatttctact cttgtagatt gaagagccag gaaacagcta tgacgctctt ctaatttcta
     6421 ctcttgtaga ttctagaaaa gaattcgata tcaagcttat cgataatcaa cctctggatt
     6481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6601 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6661 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6721 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6781 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6841 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     6901 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6961 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7021 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgcta ataaaagatc
     7081 tttattttca ttagatctgt gtgttggttt tttgtgtcac gtgcggaccg agcggccgca
     7141 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     7201 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     7261 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7321 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     7381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     7441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7561 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7681 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     7741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7801 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     7861 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     7921 gcttacagac aagctgtgac cttctccggg agctgcatgt gtcagaggtt ttcaccgtca
     7981 tcaccgaaac gcgcgatacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9841 cggttcctgg ccttttgctg gccttttgct cacatgt
//
