LOCUS       sequence-258287-        5748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfected into cell of interest for membrane expression. The 
            encoded mgSrtA could enable labeling of the interacting cells which 
            is useful for recording cell-cell interactions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ge Y, Chen L, Liu S, Zhao J, Zhang H, Chen PR
  TITLE     Enzyme-Mediated Intercellular Proximity Labeling for Detecting 
            Cell-Cell Interactions.
  JOURNAL   J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 
            10.1021/jacs.8b10286. Epub 2019 Jan 29.
  PUBMED    30676735
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.8b10286"; journalName: "J Am Chem Soc"; date: 
            "2019-02-6- 6"; volume: "141"; issue: "5"; pages: "1833-1837"
COMMENT     Sequence Label: pDisplay-mgSrtA
FEATURES             Location/Qualifiers
     source          1..5748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             409..555
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(577..594)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    583..807
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(939..1527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1019..1038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1856..1903
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1928..1947
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2135..2929)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2135..2929)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2246..2265)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2856..2875
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(3028..3047)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        3126..3317
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     3274..3294
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(3377..4168,4169..4237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4000..4019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4238..4342)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4369..4824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4456..4475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4666..4687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        4836..5215
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5216..5419
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5370..5390
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5464..5483
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5464..5482
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     5563..5622
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             5623..5646
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccggacccg ccacaagaga acaactgaac agaggagtgt ccttcgccaa agaaaaccag
       61 agcctggacg accaaaacat cagcatcgcc ggccacacct tcatcggaag acccaactac
      121 cagttcacca acctgaaggc cgccaaaaag ggaagcatgg tgtacttcaa ggtgggcaac
      181 gaaaccagaa aatacaagat gacctccata agaaacgtga agcccaccgc cgtgggcgtc
      241 ctggacgaac aaaagggaaa ggacaagcag ctgaccctga tcacctgcga tgatctgaac
      301 agagaaaccg gcgtgtggga gaccagaaaa atcctggtgg ccacagaagt gaagggatct
      361 acaagcggga gcgggaagcc cggcagcgga gaaggaagca caaagggagc tgtgggccag
      421 gacacgcagg aggtcatcgt ggtgccacac tccttgccct ttaaggtggt ggtgatctca
      481 gccatcctgg ccctggtggt gctcaccatc atctccctta tcatcctcat catgctttgg
      541 cagaagaagc cacgttaggc ggccgctcga gatcagcctc gactgtgcct tctagttgcc
      601 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      661 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      721 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      781 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
      841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1681 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     1741 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     1801 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     1861 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     1921 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     1981 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     2041 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     2101 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     2161 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     2221 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     2281 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     2341 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     2401 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     2461 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     2521 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     2581 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     2641 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     2701 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     2761 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     2821 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     2881 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     2941 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     3001 tacttctgga atagctcaga ggccgggcct cggcctctgc ataaataaaa aaaattagtc
     3061 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     3121 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     3181 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     3241 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     3301 ggttctttcc gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt
     3361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     4381 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4441 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4501 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4561 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4621 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4681 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4741 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4801 acaaaatatt aacgcttaca atttacgcgc gcgttgacat tgattattga ctagttatta
     4861 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     4921 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     4981 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5041 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     5101 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5161 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     5221 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     5281 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     5341 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     5401 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     5461 aattaatacg actcactata gggagaccca agcttggtac cgagctcgga tccactagta
     5521 acggccgcca gtgtgctgga attcggcttg gggatatcca ccatggagac agacacactc
     5581 ctgctatggg tactgctgct ctgggttcca ggttccactg gtgactacaa ggacgacgac
     5641 gacaagggaa gcggactgga gcaggcaaag ccacagatac ccaaggacaa gagcaaggtg
     5701 gcaggataca tagagatacc agacgctgac ataaaagagc ccgtgtac
//
