LOCUS       sequence-258300-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting in marmoset cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, 
            Kishi N, Shiozawa S, Okano H
  TITLE     Robust and efficient knock-in in embryonic stem cells and 
            early-stage embryos of the common marmoset using the CRISPR-Cas9 
            system.
  JOURNAL   Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w.
  PUBMED    30728412
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-6"; volume: "9(1):1528. doi"; pages: " 
            10.1038/s41598-018-37990-w"
COMMENT     Sequence Label: pKI-cjACTB-EGFP-5'3'short
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(1848..1881)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1909..2482
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1932..2484
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2076..2093)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2303..2322
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      2502..2511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2508..2510,2511..2513,2514..3224)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2508..2510,2511..2513,2514..3224)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2553..2574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2814..2833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3161..3182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3249..3314
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3321..4115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(4133..4166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(6005..6024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6006..6024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6031..6048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6031..6047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6040..6062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6185..6487
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6366..6385
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6836..7630
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6836..7630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      7830..7839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7836..8210
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8348..8936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8837..8856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9090..9107
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcagatctg
      301 gcaccacact ttttacaacg agctgcgtgt ggctcctgag gagcaccccg tgctgcttac
      361 tgaggccccc ctgaacccca aggccaaccg tgagaagatg acccaggtga gtgatctgcc
      421 acctcccctg gtggccgcct ccctccttcc tggcctcccg gagctgcgcc ctttctcact
      481 ggttctctct tctgccgttc tccgtaggac tctcttctct gacctgagtc tcctttggaa
      541 ctctgcaggt tctatttgct ttttcccaga tgaggtcttt ctggtgtttg cctttctgac
      601 taggtgtcta aaacagacag tgctgtgggt gtaggtacta acactggctc gtgtgacaag
      661 cccatgaggc tggtgtaaag cagtcttgga gtgtgtatac agtaggcgca cagtaggtct
      721 gaacagaatc cccatcccaa gaccccagca caactagcgg tgttcttgca cttactgcat
      781 gtcccctgtc tgctcttggc tgtccctagt gacttcctca gtgtgacacg gtgcatctct
      841 ttctacagat catgtttgag accttcaaca ccccagccat gtatgtggcc atccaggctg
      901 tgctttcctt gtacgcctct ggccgtacca ctggcatcgt gatggactcc ggtgacgggg
      961 tcacccacac tgtgcccatc tatgaggggt atgccctccc ccacgccatc ctacgtctgg
     1021 acctggctgg ccgggacctg actgactacc tcatgaagat cctcaccgag cgcggctaca
     1081 gcttcaccac cacagctgag cgggaaatcg tgcgtgacat caaggagaag ctgtgctacg
     1141 tcgccctgga cttcgagcag gagatggcca cggcggcctc cagctcctcc ctggagaaga
     1201 gctatgagct gcccgatggt caggtcatca ccatcggcaa cgagcggttc cgctgccctg
     1261 aggcactctt ccagccttct ttcctgggta agtggagacc actgcctagc cctgcctgac
     1321 atgaggttac cttggggctg tgctgtggaa gcttaagtct gggtctcatt tccccctcag
     1381 gcatggaatc ttgtggcatc cacgaaacta cctttaactc catcatgaaa tgtgacgtgg
     1441 acatccgcaa agacctgtat gccaacacag tgctgtctgg tggtaccacc atgtaccctg
     1501 gcatcgctga caggatgcag aaggagatca ccgccctggc acccagcaca atgaagatca
     1561 aggtgggtgt ccttccagcc tgagctgacc tgggcagggg gcatggggcc ctgtggtttg
     1621 tggggagctg tcatatcaag ggtcctcact gcctgttccc tccactcctc agatcattgc
     1681 tcctcccgag cgcaagtact cagtgtggat tggtggctcc atcctggcct cgctgtccac
     1741 cttccagcag atgtggatca gcaagcagga gtatgatgag tccggcccct ccatcgtcca
     1801 ccgcaaatgc ttctaggcgg actgtgactt agtgaattcg cccttggata acttcgtata
     1861 gcatacatta tacgaagtta tagatctccc ttttttttcc acagtccgcc cctctccctc
     1921 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     1981 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     2041 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     2101 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     2161 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     2221 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     2281 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     2341 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     2401 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     2461 cctttgaaaa acacgatgat aatatggcca cggatctgct agccaccatg gtgagcaagg
     2521 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2581 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2641 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2701 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2761 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2821 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2881 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2941 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3001 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3061 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3121 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3181 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct cgcgctaagc
     3241 gggcaccggt gaaacagact ttgaattttg accttctcaa gttggcggga gacgtggagt
     3301 ccaacccagg gccggatatc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3361 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3421 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3481 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3541 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3601 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3661 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3721 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3781 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3841 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     3901 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3961 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4021 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4081 tcgccttcta tcgccttctt gacgagttct tctgagcggc cggccgctcg acataacttc
     4141 gtataggata ctttatacga agttatgata tccatcacac tggcggccgc gggcttacct
     4201 gtacactgac ttgagaccag ttgaataaag tgcacacctt aaaaatgagg ccaagcatga
     4261 ctttgtgtcg ggctggggtg tgggcagcag gtgaacccag gggaagggtg gggcagtggg
     4321 ggccagcctg tccctactga gtggaggtgg gctgaggttc tctctccttg acattgagca
     4381 gccttagaat gtgagggaag tttaagattc aggcctctgc tcattgggga gttcctggct
     4441 tggccctgcc acctgtccct aggtacccca gtttctctgg gttcatccag aaggtctaga
     4501 tgctcaggaa gtgctggttg gcacctgtgt ttcttaggag tggagatccc ttagggtcac
     4561 tccgggcagg gggttcttga gtgggagtgt agtgggtgtt cctagctgcc atgcctttgt
     4621 cctgctcact gtattggagt ggagtcgtcc ataggctgag gagaagccat gactcctgct
     4681 tctctaccca gacagggaaa tctgggatca cagtcgaatt gtggtggggg atccatgggg
     4741 ccatgtggtt ggcaggcagc tctggttagc tctggactga gctggattct gccttgtgac
     4801 gagtaggcgc catcctgtgg cctctcaggg cccagtttgt gacccatgct gcagagggag
     4861 atggccagac tcctggacag cagggcatgc ctggcattgc aggtcagatt cttttttttt
     4921 tgagatgggg tctggctttg ttgctcaggc tttagttcag tggtacaacc acagctcact
     4981 gcagcctcag ccttctgggc aagcagttct gcctcagcct cccaaatagc tggaactaca
     5041 caggcacacc cagctagttg tgggtcagat tctcacttgt gcagagctga gtcacttttg
     5101 aaagaaacaa aacctggctt cccttccgct gctagaagct ttgattgata gtagcggaga
     5161 atgaaggaag gagacgaggc tcccttgcgg ggataccctt taccaaaaac accacctggt
     5221 gtatcacagt tgttctggaa atgatctggt ccccaggagg cctggactgc ctctcacttg
     5281 tgcacagtgg ctgctggcag agaggaaggt tgtccctttc tgtgttatct tggaggttcc
     5341 ccgaagggtc tgccaagagc agttctagct cttttggtct ttactgagtg accactagat
     5401 ggtgggcctg aagctactga gatgacctgg acaagaaccc tgccttggag gagctgtcaa
     5461 tctggaaatg aggacttcat tcatttgttc attccttgat ccatttctcc attgaatcac
     5521 tatttgtgct atgtactgtg ggtatggagg agaaccaaac agatgtgttc ctgtcctcac
     5581 caggcaccca atttaaaggg gaagacatca taaacacaaa ggtacacaaa tgagtgtcca
     5641 atgacacggt gtcatcatgg gaagaaaaag cagaggcttt gatgaacacg atcattaggt
     5701 gatattcatt atgttgattg tggtggtcta gacataaatt aacacttatc taggctgggc
     5761 gtggtggctc atgcctgtaa tcccagcact ttgggaggct gaggtgggtg gatcaagaga
     5821 tcgagaccat cctggccaac atagtgaaac cccatcttta ctaaaaatac aaaaattagc
     5881 tgggcgtggt ggtgcacact tgggaggctg aggtaggaga actgcttgaa ctggggaggc
     5941 gaacgttgca gtgagctaag attgtgctac tgtactccag cctggtgcta gagggcccaa
     6001 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     6061 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt
     6181 acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     6241 acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg
     6301 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     6361 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     6421 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     6481 aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     6541 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     6601 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     6661 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     6721 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     6781 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     6841 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6901 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6961 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     7021 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7081 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7141 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7201 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7261 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7321 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     7381 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7441 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7501 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7561 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7621 gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     7681 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7801 aaatgcttca ataatagcac gtgaggaggg ccaccatggc caagttgacc agtgccgttc
     7861 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7921 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7981 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     8041 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     8101 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     8161 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctaa
     8221 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8281 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8341 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8401 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8461 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8521 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8581 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8641 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8701 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8761 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8821 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8881 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8941 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9001 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9061 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
