LOCUS       sequence-258301-        8158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Hygro)
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..351
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     324..348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             392..448
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        476..986
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     905..923
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1004..2026
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1004..2026
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2042..2095
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2096..2713,2714..2719,2720..2755,2756..2758,2759..2794,
                     2795..2797,2798..2833,2834..2842)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2882..2902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3300..3311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3516..3749
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3821..3942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3858..3877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3912..3931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3982..4117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4117..4188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4189..4257,4258..5049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4407..4426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5220..5808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5709..5728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5962..5979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6096..6117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6132..6137,6138..6155,6156..6162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6170..6186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6175..6197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6194..6210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6194..6210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6229..6249
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6231..6249
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6277..6503
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6504..6684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6731..6856
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7349..7582
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7767..7811
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8024..8141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta tcagtgatag
       61 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
      241 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
      301 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
      361 ggctagcgaa ttcgtcgaca ccggttccgg agccacgaac ttctctctgt taaagcaagc
      421 aggagacgtg gaagaaaacc ccggtcctag gctgcagacg cgttgtacag gatccggggt
      481 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
      541 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
      601 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
      661 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
      721 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
      781 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
      841 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
      901 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
      961 accgaatcac cgacctctct ccccagcaat tcacccgcgc gcgatgaaaa agcctgaact
     1021 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     1081 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     1141 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     1201 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat ttagcgagag
     1261 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     1321 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     1381 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     1441 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     1501 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     1561 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     1621 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     1681 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     1741 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     1801 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     1861 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     1921 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     1981 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaattcg aaggaagcgg
     2041 agaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc
     2101 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat
     2161 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     2221 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     2281 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     2341 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     2401 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     2461 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     2521 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     2581 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     2641 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     2701 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
     2761 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     2821 ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact
     2881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3241 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3361 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     3421 tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt
     3481 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3541 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     3781 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     3841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     3961 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4081 ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg
     4141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5101 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5881 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5941 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6001 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6061 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6181 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6241 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6301 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6361 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6421 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6481 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6541 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6601 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6661 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6721 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6781 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6841 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6901 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6961 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7021 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7081 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7141 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7201 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7261 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7321 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7381 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7441 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7501 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7561 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7621 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7681 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7741 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7801 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7861 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7921 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7981 gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     8041 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     8101 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacaggga
//
