LOCUS       sequence-25832-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho 
            KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, 
            Sarpong R, Keasling JD
  TITLE     Production of the antimalarial drug precursor artemisinic acid in 
            engineered yeast.
  JOURNAL   Nature. 2006 Apr 13. 440(7086):940-3.
  PUBMED    16612385
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 13. 440(7086):940-3."
COMMENT     Sequence Label: pESC-leu2d(empty)
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..72
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      102..291
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(363..380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(614..633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accggacgta tcgactcact atagggcccg ggcgtcgaca tggaacagaa gttgatttcc
       61 gaagaagacc tcgagtaagc ttggtaccgc ggctagctaa gatccgctct aaccgaaaag
      121 gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta
      181 ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca
      241 tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa gatccagctg
      301 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gc
//
