LOCUS       sequence-25833-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Gitler lab plasmids
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCRII-zSynB
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(583..602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(584..602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(609..625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(618..640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cncagctggt ccgagctcgg atccctagta acggccgcca gtgtgctgga attcgccctt
       61 atatagtatc tttgctcaca gtaagtatcc aactcatcac catcatcatc atctttctaa
      121 cctcattgca ctgggtggag gaaactggtc aggtaagaaa gcctccagtt tcagggcagg
      181 taagaaaggc gactttgtct tcatgtaaat ctccaaatca caagcacatg catacgaaat
      241 ctgcacaaat gtgagaatga cacgaaccgc acattataca tatcttatta atattgcata
      301 gctagatagt gttacacaac aatactattt cagtatatct atatgtggcg cgtgaggtaa
      361 gtaaaggctg attgtgagga gaaagcaaga gacgagtgtg tttgttttct gaagttgaag
      421 attatttgtt gttcttcaga ggtttaacat gatgatactc agcagctaat aaactcttct
      481 ctcaggcaag aagcagagca tcatgggaca ttcaagggcg aattctgcag atatccatca
      541 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat
      601 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      661 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      721 accgatcgcc cttcccaaca gttgcgcagc ctgatggana angnancccc cntgnaccgc
      781 ncatanncnc gcgggngtgg ggtacccnca cgggc
//
