LOCUS       sequence-258335-       14132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting in marmoset cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, 
            Kishi N, Shiozawa S, Okano H
  TITLE     Robust and efficient knock-in in embryonic stem cells and 
            early-stage embryos of the common marmoset using the CRISPR-Cas9 
            system.
  JOURNAL   Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w.
  PUBMED    30728412
REFERENCE   2  (bases 1 to 14132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-6"; volume: "9(1):1528. doi"; pages: " 
            10.1038/s41598-018-37990-w"
COMMENT     Sequence Label: pKI-cjPLP1e5-P216S
FEATURES             Location/Qualifiers
     source          1..14132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..54
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2861..2894
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3091..3590
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3604..4407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3667..3686)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4277..4296
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    4721..4754
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9017..9516
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     5'UTR           9558..9597
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             9615..10745
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(10743..10762)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10787..10835
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(11353..11372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11354..11372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11382..11399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11382..11398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11391..11413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11540..11995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11627..11646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11837..11858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12021..12125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12126..12194,12195..12986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12344..12363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13157..13745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13646..13665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13899..13916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14033..14054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14069..14074,14075..14092,14093..14099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14107..14123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(14112..14132,1..2)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(14131..14132,1..15)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(14131..14132,1..15)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
       61 aagctgggta cggccaatgc ggccgctaga gaatccctag ctttgttagg gtgcctgctc
      121 tggcgtatac cacatttatg tgagtaaagg agctgggtct tcaattaata cgattccttc
      181 gtctctttca tctccctgtt actgcaggat ccatgccttc cagtatgtca tctatggaac
      241 tgcctctttc ttcttccttt atggggccct cctgctggct gagggcttct acaccaccgg
      301 cgcagtcagg cagatctttg gcgactacaa gaccaccatc tgcggcaagg gcctgagcgc
      361 aacggtaaca gggggccaga aggggagggg ttccagaggc caacatcaag ctcattcttt
      421 ggagcgggtg tgtcattgtt tgggaaaatg gctaggacat cccgacaagg tgatcatcct
      481 caggattttg tggcaataac aaggggtggg ggaaaattgg gcgtgagtct gtggcctcgt
      541 ccccacccaa ggctgggtcc tctctagggg cccggcattt gagtgaggaa gcgatggctg
      601 cagccgaacg agaagatcag gaagaacgtg gtgcccagct ggcttagcct cacctttcaa
      661 aggttcccta agcaaatttc ttttcaaaat ggaaagcatg agttatgtag gatgctttgt
      721 acaatcagac catttctaag ccacctgttg gtatcccttt actcccttcc tagcagggac
      781 cacaagagtg gatataactg gacaagagtc taaaatactg ctcgtgagat tgagacttgg
      841 acacctgatc tgagaaggag gattgataat aaaaattaaa taattgactc caaggtgaaa
      901 tttaccatat tccgggatcc tactctgttg gaagtcccag agggaattgg agtagatctg
      961 catgttgaat gttttatcat tgtctgaggg ttatccatct gtgctttgag tggggtgcac
     1021 attggttttc agtatctgga gttaaagatc ctttgagggc tgaacatgtg caggttctag
     1081 ccatgtcttt ttgtggccag cagtcagctt agaaaggttt tgtatatgaa attttaatct
     1141 cctattggct ttgttcaatg actagggaac aaaaatgttc ccgtggcaag gaacattttc
     1201 taagctcagc ctaaggcaca aaacggcacg actttctttc aagatctatt ttgatttctt
     1261 tacaccttat ctgcccagaa acatctcctg aagcctttct aacccaggga tcctcctcac
     1321 tcctccccta cccattcccc ccaccctccg tcacacttgg ggccagttct ctagtagata
     1381 ctgccaatga cccttggcag aggtgccctg ctcactaatt taatttgggg gagccctgga
     1441 atctgatttt aatatctggc acatgccagt acaggatctc ccagtttgtg tttctacatc
     1501 tgcagacgga tactgatttc taaccacccc atgtcaatca ttttagtttg tgggcatcac
     1561 ctatgccctg accgttgtgt ggctcctggt gtttgcctgc tctgctatac ctgtgtacat
     1621 ttacttcaac acctggacca cctgccagtc tattgccttc cccagcaaga cctctgccag
     1681 tataggcagt ctctgtgctg atgccagaat gtatggtgag ttagggcacg ggtgctttgg
     1741 ctctcctacc cactatggaa gcacaatata tttggttatt ttgttagtgt aaggagggtg
     1801 gtgattatga gaaaaagata agatgatgaa tgcttgggtc ttagtttatt aatccttccc
     1861 tactgaaacc agagaggttt attccctgcg aagggaactt ggaggcgatg ggaattttct
     1921 tggccattca cattggccta ctctagttaa ttgctgttct gaaccccaaa gcagcaaatt
     1981 tcaataacaa atacaaagtt gaagcagtgt tcaatactgt cttctctttt ccgtaaacct
     2041 gtagaaaaga ggatcctgat tgttggtaga atccaacttt acagcaagga taattagaga
     2101 tggaagaagg gctctacggg gaagtctcca catggcccat aactctataa agcttcccct
     2161 ggttgatttt tgtgtcttac ttaggtgttc tcccatggaa ttctttctct ggcaaggttt
     2221 gtggctccaa ccttctatcc atctgcaaaa cagctgaggt gagtgcgtta tttgtgttat
     2281 tttgcaacag agtgtgtaat accatacaaa ttatacccat agccttcaat ttcaaggact
     2341 aaaagtttct ctttgctgga ttttgaatta ccagattgcc ttctaaaaaa tattggctaa
     2401 gtatgcctga gccaatgagc acagaaagta aaacacctgt tttccctaga gccacataga
     2461 aagacttccc atcttaaaag agaagtttgt aactttaggt aaagatagag cctaatggca
     2521 aaatccccta gatgaaaatt ttcattcctt tagttaaaca ccatatcaag aaaatgaact
     2581 gtgcacagag acattcatag tttttataaa aacattttca gtttggaact ttttttaatc
     2641 tcaaatgaga ggtaaaataa gtacatttga agaggaaagc actgtattga gaatagatat
     2701 tataaacaaa tgagtcaggg gactaaaagt aagccctggg gagaaaggca gtaaattatt
     2761 tctttggaaa caaaatggtg actttaaatt ttgggcccct agcggtaccg ggccccccct
     2821 cgaccgccgc taattccgat catattcaat aacccttaat ataacttcgt ataatgtatg
     2881 ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagcttt aatgcggtag
     2941 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
     3001 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
     3061 actgccgggc ctcttgcggg gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3121 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     3181 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     3241 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3301 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3361 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3421 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3481 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3541 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3601 aatatgggat cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3661 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3721 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3781 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3841 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3901 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3961 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4021 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4081 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4141 cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4201 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4261 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4321 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4381 tatcgccttc ttgacgagtt cttctgaggg gatccgctgt aagtctgcag aaattgatga
     4441 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
     4501 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
     4561 tggggtggga ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga
     4621 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
     4681 attcctccca ctcatgatct atagatccgg aacccttaat ataacttcgt ataatgtatg
     4741 ctatacgaag ttattaggtc cctcgactct aggcacgact gtagggaacc agtatttggg
     4801 agcactgtac tatttcatta gtattctgtg ttctgggtgt tatggggcca ggtgctatga
     4861 tgtacactga agactgggag gcccacactt agggaagtga aaacttagtt agagatgtag
     4921 aatttgcata caagaaaggt atcagcacat tcagaaagct atattcatga tttacagtgg
     4981 ggcatattat tactattgca agaaacagtt cttcctcttg cattttcctg cagttccaaa
     5041 tgaccttcca cctgtttatt gctgcatttg tgggggctgc agctactctg gtttccctgg
     5101 tgagttgact ttgaatgatc ttggcaagta aataggcctg agacagtgtg ggtacagcta
     5161 ttctgaaagg caagaagaca gtctgtttcc atccttgaaa tgctggagag aagcttctgg
     5221 gaagatagcg aagggtagag gctctgtact tttacttgca tagtcaaggt ggaaaaacat
     5281 gacaaattag ataggaacaa gaaaagtggt atgaggaaag aggaggtaag agattcatag
     5341 acacgaatga taatatttag tatttgtaga ccgtgtaatg ttgccaagtg ctttgcagtc
     5401 ataaattacg gttaattctg aatacgttgt gatcagatca gtgacaacac actgaaacat
     5461 gaaacatgac ttgctatagc ctggaattct atatactatc aatacgaatc taccttggat
     5521 actctccaat ggatttagtt actcatgtgg aaatgccggg aggacctcct aacattatca
     5581 gaattgttat gactgtaata caatgctatg tcccaggtct tgctgatagt gttacagtga
     5641 cctgtgaatg tagttgaatg tagtgtgctc attgtgcaga tcagaaacct aaggcactga
     5701 agggtgaagt gatttatctg aagtcattgt ataaacagtg atcagacaag atgagctcac
     5761 agaactcctg gcccctattg ctgaggcttc tatacagact taagtaattt ctcgccttgt
     5821 aaaatgaatt gattcattaa gcaggggagg gctatactgc atgatgtagc tgtgtgtcta
     5881 cagcaagcat cctatgactc caagtcactc agacatgtgg atgtggcaaa gcccaaatac
     5941 tgttctcttc cccgatgaaa actcagtact agacaagagg tgtggaataa atctttatga
     6001 tttgattctc tgggcctggg ccattggaag gaaatgctgc agagacatcg gacttccagt
     6061 caagtgtata tggagaaagc caagcctggg atatactgct ttttgcagaa caggggtttt
     6121 ccccttattt agttatgatt ttatttcatc cttcctcatt cccaaaggga tttgaggagg
     6181 gagcgttttc ttttctactc tcattaacat tctctattct gttccctaca gctcaccttc
     6241 atgattgctg ccacttacaa ctttgccgtc cttaaactca tgggccgagg caccaagttc
     6301 tgatcccccg taaaaatccc cctttctcta atagcgaggc tctaaccaca cagccaacaa
     6361 tactgcgtct cccatcttaa ctctttgcct ttgccactga ctggccctct tcttacatga
     6421 tgagtgtaac aagaaaggag agtcttgcag tgattaaggt ctctctgtgg actctcccct
     6481 cttatgtacc tcttttagtc attttgcttc acagctggtt cctgctagaa atgggaaatg
     6541 cctaagaaga tgactcccca actgcaagtc acaaaggaat ggaggctcta attcaatttt
     6601 caagcatctc ctgaggatca gaaagtaatt tcttctcaaa gggtgcttcc actgatggaa
     6661 acaaagtggg aggaaagatg ctcaggtaca gagaaggaat gtctttggtc cccttgccat
     6721 ctgtaggggc caaatatatt ctctttagtg tacaaaacag agaactcact ctggtctgcc
     6781 tattaccact gaagatagaa gaaaaatgaa tgtcagaaaa cccagtaaga gcgtttgccc
     6841 aaatctgcct attgcagctg ggagaagggg gtcaaagcaa ggatctttca ctcacagaaa
     6901 gagagcactg accccggtgg cgatggacta ccaaagccct aattcagcca accttactta
     6961 cagcataagg gagcgtagtg tctgtgtaga cgaagggggc atctggcctt acacctcgtt
     7021 agggaagaga aacagggtgt tgtcagcatc tttttactcc cttcttcttg ataacagcta
     7081 ccatgacaac cccgtggttt ccaaggagct gagaatagaa ggaaactagc ttacatgaaa
     7141 acagactggc ctaaggagca gcagttgctg gtggctaatg gtgtaacctg agatggccct
     7201 ctggtagaca caggatagat aactctttgg atagcatgtc tttttttctg ttaattagtt
     7261 gtgtactctg gcctctgtta tatcttcaca atggtgctct tttcatgggg tattatccat
     7321 ttagtcatcg gaggtgattt gaagatcttg atgtgtttta gaatgatgca cattttgtgt
     7381 attccaattt gtttattact tatttggggt tgcatcagaa atgtctggag aataattctt
     7441 tgattatgac tcttttttaa aataggaaaa ttggacatta agtattacaa atgatattaa
     7501 acattggcta gttagatgaa ccaacctatt gcgattttct acaagtattc tgcctttgca
     7561 gaaacagatt tggtgaattt gaatctcaat ttgagtaatc tgcttgattg ttctttctag
     7621 ctaatggaaa atgattttac ttgcaatgtt atcttggtgt gttaagagtt aggtttaaca
     7681 taaaggttat tttctcctga aatagatcgc ataacagaat gcaccagtca tcagctattc
     7741 agttggtaag cttccaggaa aaaggacagg cagaaagagt ttgaaacctg aatagctccc
     7801 agattttagt cttttcctgt ttttgttaac tttgggttta aacaaaagtc tgattggttt
     7861 taattgaatg acagatttgt actacagctc ttttgttgta aagagttgtg ttgttctttt
     7921 cccccaaagt ggtctcagca atatttaagg agatgtaaga tctttacaaa aagatacttg
     7981 atacttgttt tcaaatcagt atacaagata agcttccagg ctgcacagag ggaggagagg
     8041 gaaagtgttt tgtaagaaac caatcaagat caagaacagt gaagtaatcc gtaccttgtg
     8101 ttttgttttg atttaataac ataacgaata accaaccctt ccctgaaaac ctcacatgca
     8161 tacatacaca tatatacaca cacaaagaga gttaatcaac tgaaagtatt tccttcattt
     8221 ctgatataga attgcaattt taacacacat aaaggataaa cttttagaaa cttatcttac
     8281 aaagtgtatt ttataaaatt aaagaaaata aaattaagaa tgttctcaat caaatatcgt
     8341 gtcctttgtg tgaattgttc tatttgactt cacaataaaa agttaataat tgtaccttgt
     8401 tcaaggagct cattcatttt tcaggttatc caagtcattc tcataacatt tttctgaaat
     8461 aaatagtaga tgaatagatt acgtctattt taatagttaa agacactaag aaatagaagc
     8521 aaagtaattt gcccaaagaa atccagtaag tacatgtctg agcttgggtt agaatgcaga
     8581 tattgaaacc aatttattgc ctacttaggt ttattttctc ttcaaagttg ggtttcagag
     8641 tgttcagagc caactatggc tagtttttaa taccatagtg gtgccccagt ccctggtgca
     8701 tttagatttc gatttaggct attgccacct tctctttaaa ttgtttggat atttcagtaa
     8761 tgatccctta gtacttcata catgactaat atttagtttt ctctggatag tactggttgg
     8821 aagatttcat gtttaaagga cagagcaggt atgaaaaggg caatgcaaat gtaacaggct
     8881 gtggcagggc agtactaaaa taagttagtg acacctacca ccctggatgg ccagttcact
     8941 agcccactgg agacatagca gtaaagccct ttcacagccc tagaatcccg ggtaatgcat
     9001 ctcgaggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     9061 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     9121 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     9181 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     9241 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     9301 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     9361 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     9421 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     9481 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcgaccc gcttaacagc
     9541 gtcaacagcg tgccgcagat cttggtggcg tgaaactccc gcacctcttc ggcaagcgcc
     9601 ttgtagaagc gcgtatggct tcgtacccct gccatcaaca cgcgtctgcg ttcgaccagg
     9661 ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt gcgccctcgc cggcagcaag
     9721 aagccacgga agtccgcctg gagcagaaaa tgcccacgct actgcgggtt tatatagacg
     9781 gtcctcacgg gatggggaaa accaccacca cgcaactgct ggtggccctg ggttcgcgcg
     9841 acgatatcgt ctacgtaccc gagccgatga cttactggca ggtgctgggg gcttccgaga
     9901 caatcgcgaa catctacacc acacaacacc gcctcgacca gggtgagata tcggccgggg
     9961 acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat gccgtgaccg
    10021 acgccgttct ggctcctcat atcggggggg aggctgggag ctcacatgcc ccgcccccgg
    10081 ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac ccggccgcgc
    10141 gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc ctcatcccgc
    10201 cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga cacatcgacc
    10261 gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg gccgcgattc
    10321 gccgcgttta cgggctgctt gccaatacgg tgcggtatct gcagggcggc gggtcgtggc
    10381 gggaggattg gggacagctt tcggggacgg ccgtgccgcc ccagggtgcc gagccccaga
    10441 gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt cgggcccccg
    10501 agttgctggc ccccaacggc gacctgtaca acgtgtttgc ctgggccttg gacgtcttgg
    10561 ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg cccgccggct
    10621 gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc accacccccg
    10681 gctccatacc gacgatctgc gacctggcgc gcacgtttgc ccgggagatg ggggaggcta
    10741 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    10801 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    10861 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    10921 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    10981 ggcggcaagc cctgccatag ccacgggccc cgtgggttag ggacggggtc ccccatgggg
    11041 aatggtttat ggttcgtggg ggttattatt ttgggcgttg cgtggggtca ggtccacgac
    11101 tggactgagc agacagaccc atggtttttg gatggcctgg gcatggaccg catgtactgg
    11161 cgcgacacga acaccgggcg tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg
    11221 cgcggatttc tggcgccgcc ggacgaacta aacctgacta cggcatctct gccccttctt
    11281 cgctggtacg aggagcgctt ttgttttgta ttggtcacca cggccgagtt tccgcggtgg
    11341 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    11401 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    11461 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    11521 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    11581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    11641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    11701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    11761 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    11821 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    11881 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    11941 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    12001 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    12061 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    12121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    12181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    12241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    12301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    12361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    12421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    12481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    12541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    12601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    12661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    12721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    12781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    12841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    12901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    12961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    13021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    13081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    13141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    13201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    13261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    13321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    13381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    13441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    13501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    13561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    13621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    13681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    13741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    13801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    13861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    13921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    13981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    14041 gttagctcac tcattaggca ccccaggctt tacactttat gctcccggct cgtatgttgt
    14101 gtggaattgt gagcggataa caatttcaca ca
//
