LOCUS       sequence-25834-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Gitler lab plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCRII-zSynC
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(546..565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(572..589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(572..588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(581..603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cnagctggtc cgagctcgga tccctagtaa cggccgccag tgtgctggaa ttcgcccttc
       61 cgaggcgtaa aagcgtcaca ttccaagccc aaatccagcc agcagcacaa aaagtttata
      121 tcaataccaa gtacaaatcc taaacacaat aaaaacctgt tgcaaaaaaa acaaaaaaaa
      181 ggtgaaaaaa atccaaaaaa cgtaaatcta tattccagtt aatcctgccg agaaatgttc
      241 ttcctatcag atatctactt ttatgatgat ctattgtaaa tgtcatgttc tgacggtaac
      301 gtgttggatg tagcgctatg ctaactgccg gaggactctc cgtctcgttt ccttgaatcc
      361 ctcccaatga aacgagaccc gttctccatc gtgaatcttt gtactcgtga acctgcattt
      421 ctatatgtgt aatagatgtt tcatatcctc caatggtata ttggggattc gaatataagg
      481 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca
      541 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      601 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      721 gcgaatggac gcgccctgta gcggcgcata cggggggggn ggggtgttcc ccnagntgnc
      781 cntacttngc agcgcctagg ccgctcttcg nttntccttc tttccgc
//
