LOCUS       sequence-258346-       11575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation 
            at H1077Y and D1079A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12052"; journalName: "Nature"; date: "2013-05-2- 2"; 
            volume: "497"; issue: "7447"; pages: "122-6"
COMMENT     Sequence Label: FH-TET3-HxDmut-pEF
FEATURES             Location/Qualifiers
     source          1..11575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1247..1256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1256..1279
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1292..1318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1292..1318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4091..4102
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             6734..6775
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6785..6802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6825..6842)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6831..7055
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7101..7529
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7188..7207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7398..7419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7538..7558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7543..7872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7723..7858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7785..7804
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7939..8733
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7993..8012)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8603..8622
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8909..9030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8946..8965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9000..9019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9079..9095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9079..9095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9092..9114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9127..9133,9134..9151,9152..9157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9470..10058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9550..9569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10229..11020,11021..11089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10852..10871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11090..11194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11269..11288)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgcca ccatggacta
     1261 caaggacgac gatgacaagc tcgatggagg atacccctac gacgtgcccg actacgccgg
     1321 aggactcgac agccagtttc aggtgcccct ggccgtccag ccggacctgc caggccttta
     1381 tgacttccct cagcgccagg tgatggtagg gagcttcccg gggtctgggc tctccatggc
     1441 tgggagtgag tcccaactcc gagggggtgg agatggtcga aagaaacgga aacggtgtgg
     1501 tacttgtgag ccctgccggc ggctggaaaa ctgtggcgct tgcactagct gtaccaaccg
     1561 ccgcacgcac cagatctgca aactgcgaaa atgtgaggtg ctgaagaaaa aagtagggct
     1621 tctcaaggag gtggaaataa aggctggtga aggagccggg ccgtggggac aaggagcggc
     1681 tgtcaagaca ggctcagagc tcagcccagt tgatggacct gttccaggtc agatggactc
     1741 agggccagtg taccatgggg actcacggca gctaagcgcc tcaggggtgc cggtcaatgg
     1801 tgctagagag cccgctggac ccagtctgct ggggactggg ggtccttggc gggtagacca
     1861 aaagcccgac tgggaggctg ccccaggccc agctcatact gctcgcctgg aagatgccca
     1921 cgatctggtg gccttttcgg ctgtggccga agctgtgtcc tcttatgggg cccttagcac
     1981 ccggctctat gaaaccttca accgtgagat gagtcgtgag gctgggaaca acagcagggg
     2041 accccggcca gggcctgagg gctgctctgc tggcagcgaa gaccttgaca cactgcagac
     2101 ggccctggcc ctcgcgcggc atggtatgaa accacccaac tgcaactgcg atggcccaga
     2161 atgccctgac tacctcgagt ggctggaggg gaagatcaag tctgtggtca tggaaggagg
     2221 ggaggagcgg cccaggctcc cagggcctct gcctcctggt gaggccggcc tcccagcacc
     2281 aagcaccagg ccactcctca gctcagaggt gccccagatc tctccccaag agggcctgcc
     2341 cctgtcccag agtgccctga gcattgccaa ggaaaaaaac atcagcttgc agaccgccat
     2401 tgccattgag gccctcacac agctctcctc tgccctcccg cagccttctc attccacccc
     2461 ccaggcttct tgcccccttc ctgaggcctt gtcacctcct gcccctttca gatctcccca
     2521 gtcttacctc cgggctccct catggcctgt ggttcctcct gaagagcact catcttttgc
     2581 tcctgatagc tctgccttcc ctccagcaac tcctagaact gagttccctg aagcctgggg
     2641 cactgacacc cctccagcaa cgccccggag ctcctggccc atgcctcgcc caagccccga
     2701 tcccatggct gaactggagc agttgttggg cagcgccagt gattacatcc agtcagtatt
     2761 caagcggcct gaggccctgc ctaccaagcc caaggtcaag gtggaggcac cctcttcctc
     2821 cccggccccg gccccatccc ctgtacttca gagggaggct cccacgccat cctcggagcc
     2881 cgacacccac cagaaggccc agaccgccct gcagcagcac ctccaccaca agcgcagcct
     2941 cttcctagaa caggtgcacg acacctcctt ccctgctcct tcagagcctt ctgctcctgg
     3001 ctggtggccc ccaccaagtt cacctgtccc acggcttcca gacagaccac ccaaggagaa
     3061 gaagaagaag ctcccaacac cagctggagg tcccgtggga acggagaaag ctgcccctgg
     3121 gatcaagccc agtgtccgaa agcccattca gatcaagaag tccaggcccc gggaagcaca
     3181 gcccctcttc ccacctgtcc gacagattgt cctggaaggg cttaggtccc cagcctccca
     3241 ggaagtgcag gctcatccac cggcccctct gcctgcctca cagggctctg ctgtgcccct
     3301 gcccccagaa ccttctcttg cgctatttgc acctagtccc tccagggaca gcctgctgcc
     3361 ccctactcag gaaatgaggt cccccagccc catgacagcc ttgcagccag gctccactgg
     3421 ccctcttccc cctgccgatg acaagctgga agagctcatc cggcagtttg aggctgaatt
     3481 tggagatagc tttgggcttc ccggcccccc ttctgtgccc attcaggacc ccgagaacca
     3541 gcaaacatgt ctcccagccc ctgagagccc ctttgctacc cgttccccca agcaaatcaa
     3601 gattgagtct tcgggggctg tgactgtgct ctcaaccacc tgcttccatt cagaggaggg
     3661 aggacaggag gccacaccca ccaaggctga gaacccactc acacccaccc tcagtggctt
     3721 cttggagtca cctcttaagt acctggacac acccaccaag agtctgctgg acacacctgc
     3781 caagagagcc caggccgagt tccccacctg cgattgcgtc gaacaaatag tggagaaaga
     3841 tgaaggtcca tattatactc acttgggatc tggccccacg gtcgcctcta tccgggaact
     3901 catggaggag cggtatggag agaaggggaa agccatccgg atcgagaagg tcatctacac
     3961 ggggaaggag ggaaagagct cccgcggttg ccccattgca aagtgggtga tccgcaggca
     4021 cacgctggag gagaagctac tctgcctggt gcggcaccgg gcaggccacc actgccagaa
     4081 cgctgtgatc gtcatcctca tcctggcctg ggagggcatt ccccgtagcc tcggagacac
     4141 cctctaccag gagctcaccg acaccctccg gaagtatggg aaccccacca gccggagatg
     4201 cggcctcaac gatgaccgga cctgcgcttg ccaaggcaaa gaccccaaca cctgtggtgc
     4261 ctccttctcc tttggttgtt cctggagcat gtacttcaac ggctgcaagt atgctcggag
     4321 caagacacct cgcaagttcc gcctcgcagg ggacaatccc aaagaggaag aagtgctccg
     4381 gaagagtttc caggacctgg ccaccgaagt cgctcccctg tacaagcgac tggcccctca
     4441 ggcctatcag aaccaggtga ccaacgagga aatagcgatt gactgccgtc tggggctgaa
     4501 ggaaggacgg cccttcgcgg gggtcacggc ctgcatggac ttctgtgccc acgcctacaa
     4561 ggcccagcat aacctctaca atgggtgcac cgtggtctgc accctgacca aggaagacaa
     4621 tcgctgcgtg ggcaagattc ccgaggatga gcagctgcat gttctccccc tgtacaagat
     4681 ggccaacacg gatgagtttg gtagcgagga gaaccagaat gcaaaggtgg gcagcggagc
     4741 catccaggtg ctcaccgcct tcccccgcga ggtccgacgc ctgcccgagc ctgccaagtc
     4801 ctgccgccag cggcagctgg aagccagaaa ggcagcagcc gagaagaaga agattcagaa
     4861 ggagaagctg agcactccgg agaagatcaa gcaggaggcc ctggagctgg cgggcattac
     4921 gtcggaccca ggcctgtctc tgaagggtgg attgtcccag caaggcctga agccctccct
     4981 caaggtggag ccgcagaacc acttcagctc cttcaagtac agcggcaacg cggtggtgga
     5041 gagctactcg gtgctgggca actgccggcc ctccgaccct tacagcatga acagcgtgta
     5101 ctcctaccac tcctactatg cacagcccag cctgacctcc gtcaatggct tccactccaa
     5161 gtacgctctc ccgtctttta gctactatgg ctttccatcc agcaaccccg tcttcccctc
     5221 tcagttcctg ggtcctggtg cctgggggca tagtggcagc agtggcagtt ttgagaagaa
     5281 gccagacctc cacgctctgc acaacagcct gagcccggcc tacggtggtg ctgagtttgc
     5341 cgagctgccc agccaggctg ttcccacaga cgcccaccac cccactcctc accaccagca
     5401 gcctgcgtac ccaggcccca aggagtatct gcttcccaag gcccccctac tccactcagt
     5461 gtccagggac ccctccccct ttgcccagag ctccaactgc tacaacagat ccatcaagca
     5521 agagccagta gacccgctga cccaggctga gcctgtgccc agagacgctg gcaagatggg
     5581 caagacacct ctgtccgagg tgtctcagaa tggaggaccc agtcaccttt ggggacagta
     5641 ctcaggaggc ccaagcatgt cccccaagag gactaacggt gtgggtggca gctggggtgt
     5701 gttctcgtct ggggagagtc ctgccatcgt ccctgacaag ctcagttcct ttggggccag
     5761 ctgcctggcc ccttcccact tcacagatgg ccagtggggg ctgttccccg gtgaggggca
     5821 gcaggcagct tcccactctg gaggacggct gcgaggcaaa ccgtggagcc cctgcaagtt
     5881 tgggaacagc acctcggcct tggctgggcc cagcctgact gagaagccgt gggcgctggg
     5941 ggcaggggat ttcaactcgg ccctgaaagg tagtcctggg ttccaagaca agctgtggaa
     6001 ccccatgaaa ggagaggagg gcaggattcc agccgcaggg gccagccagc tggacagggc
     6061 ctggcagtcc tttggtctgc ccctgggatc cagcgagaag ctgtttgggg ctctgaagtc
     6121 agaggagaag ctgtgggacc ccttcagcct ggaggagggg ccggctgagg agccccccag
     6181 caagggagcg gtgaaggagg agaagggcgg tggtggtgcg gaggaggaag aggaggagct
     6241 gtggtcggac agtgaacaca acttcctgga cgagaacatc ggcggcgtgg ccgtggcccc
     6301 agcccacggc tccatcctca tcgagtgtgc ccggcgggag ctgcacgcca ccacgccgct
     6361 taagaagccc aaccgctgcc accccacccg catctcgctg gtcttctacc agcacaagaa
     6421 cctcaaccag cccaaccacg ggctggccct ctgggaagcc aagatgaagc agctggcgga
     6481 gagggcacgg gcacggcagg aggaggctgc ccggctgggc ctgggccagc aggaggccaa
     6541 gctctacggg aagaagcgca agtggggggg cactgtggtt gctgagcccc agcagaaaga
     6601 gaagaagggg gtcgtcccca cccggcaggc actggctgtg cccacagact cggcggtcac
     6661 cgtgtcctcc tatgcctaca cgaaggtcac tggcccctac agccgctgga tctagtctag
     6721 agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     6781 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6841 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6901 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6961 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7021 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7081 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7501 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7561 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7621 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7681 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7741 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7801 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7861 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     7921 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7981 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8041 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8101 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8161 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8221 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8281 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8341 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8401 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8461 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8521 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8581 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8641 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8701 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac
     8761 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     8821 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8881 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9001 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9061 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11221 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    11281 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    11341 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    11401 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
    11461 acattgatta ttgactaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac
    11521 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
