LOCUS       sequence-258386-        2495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Research the effect of gene orientation of transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Yeung E, Dy AJ, Martin KB, Ng AH, Del Vecchio D, Beck JL, Collins 
            JJ, Murray RM
  TITLE     Biophysical Constraints Arising from Compositional Context in 
            Synthetic Gene Networks.
  JOURNAL   Cell Syst. 2017 Jul 26;5(1):11-24.e12. doi: 
            10.1016/j.cels.2017.06.001. Epub 2017 Jul 19.
  PUBMED    28734826
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2017.06.001"; journalName: "Cell Syst"; date: 
            "2017-07-26- 26"; volume: "5"; issue: "1"; pages: "11-24.e12"
COMMENT     Sequence Label: pEYD1A
FEATURES             Location/Qualifiers
     source          1..2495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(607..1398,1399..1467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1230..1249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1468..1572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1640..1658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    1795..1813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1820..1838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1991..2007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2014..2030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(join(2343..2495,1..436))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2423..2442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
       61 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      121 gccagaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
      961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1201 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     1261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     1621 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattctg
     1681 gcgaatcctc tgaccaatta gtgtgtttag ggatgacaga aaagcccgcc tttcggcggg
     1741 ctttgggatc cattcgttac ctggctctcg ccagtcggga tcctgtgctc agtatctcta
     1801 tcactgatag ggatgtcaat ctctatcact gatagggatc ttcaccttaa tccattatac
     1861 ataactcata cattccaaac taatctaact tcttccatca ttaaccacac ctctctatca
     1921 tcacctattc ccctctatct ccctcaaatt ctttcacata ttatatccta acactcatac
     1981 acccatacaa ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc
     2041 acagcccgga tagctcagtc ggtagagcag cggccggacg caactgaatg aaatggtgaa
     2101 ggacgggtcc aggtgtggct gcttcggcag tgcagcttgt tgagtagagt gtgagctccg
     2161 taactagtcg cgtccggccg cgggtccagg gttcaagtcc ctgttcgggc gccacaaagc
     2221 ccgccgaaag gcgggctttt ctgttacccc cttacaaaac cacaggataa cgcaggaaag
     2281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2461 cgctctcctg ttccgaccct gccgcttacc ggata
//
