LOCUS       sequence-258452-        4469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CmR, Para-popZ-rbs-msfGFPC-H3H4. Replicative plasmid for the 
            expression of bait. C-terminal GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Lim HC, Bernhardt TG
  TITLE     A PopZ-Linked Apical Recruitment Assay for Studying Protein-Protein 
            Interactions in the Bacterial Cell Envelope.
  JOURNAL   Mol Microbiol. 2019 Sep 24. doi: 10.1111/mmi.14391.
  PUBMED    31550057
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2019 Sep 24. doi: 10.1111/mmi.14391."
COMMENT     Sequence Label: pHCL150
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..303
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     226..245
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2227..2418
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2412..2434)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2520..2660
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2575..2594
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2675..2692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2846..3434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2926..2945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4177..4196
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4263..4365)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gaattccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
       61 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
      121 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
      181 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
      241 tatccataag attagcgaat cctacctgac gctttttatc gcaactctct actgtttctc
      301 catacccgtt tttttgggtc tagcaggaga tacatatatg tccgatcagt ctcaagaacc
      361 tacaatggag gaaatcctcg cctccattcg acgcatcatc tcggaggatg acgcgccggc
      421 ggagcctgcg gccgaagcgg cgcccccgcc gccgccggaa cccgaacctg aaccggtgtc
      481 gttcgacgac gaggttctgg aattgacgga cccgatcgcg cccgagcccg agctgccgcc
      541 gctggagact gtcggcgaca tcgacgtcta ttcgccgccg gaacctgagt cggaaccggc
      601 ctacacgccg ccgccggcgg ctccggtgtt tgatcgcgac gaagtcgccg agcagctggt
      661 cggcgtttcg gccgcttcgg ccgcggcgag cgccttcggc agcctgagct cggccctgct
      721 gatgcccaag gacggtcgga cgctggaaga cgtcgtacgc gagctgctgc gcccgctgct
      781 caaggagtgg ctggaccaga acctgccgcg catcgtcgag accaaggttg aggaagaagt
      841 gcagcgtatc tctcggggac gcggctaacg ctgtctagat ttaagaagga gatatacata
      901 tgctcgaggg tggaggctct ggtggcggtt ctaagggaga agagttgttt acgggtgttg
      961 taccgattct tgtcgaactg gacggagatg tgaatggaca taaattttcg gtccgtggcg
     1021 agggagaggg tgacgctacc aacggaaaac tcactcttaa gttcatctgt accaccggca
     1081 agctgcctgt cccatggcca acccttgtga cgactctgac ttatggcgtg caatgttttt
     1141 cgcggtatcc cgaccacatg aaacagcatg attttttcaa gtcggcgatg ccagaaggat
     1201 acgtgcaaga gcggacgatc tccttcaagg atgacggcac ctacaagacc cgtgccgaag
     1261 ttaagtttga gggcgatact ttggtcaatc ggattgagct gaaaggaatc gatttcaaag
     1321 aagacggcaa tatcctggga cacaagctcg aatataattt taacagccac aatgtatata
     1381 ttactgcgga taaacagaag aatggcatca aggcaaactt taaaattcgc cataacgtag
     1441 aagatggatc ggtccaactc gccgaccact accagcagaa cactccaatc ggagatggcc
     1501 cggttttgct tccagataac cattatctca gcactcaatc aaagctcagc aaagacccca
     1561 acgagaagcg ggatcacatg gtactgttgg agttcgtaac cgctgccggt atcacgcacg
     1621 gtatggacga attgtataaa ggaggtggtt ctggtggcgg atccggaggc ggtagtgcgt
     1681 tattaatgcc taaggatggc cgcacacttg aagatgttgt tagagaactt ctgcgtcctt
     1741 tgcttaaaga atggcttgat caaaatttgc ctcgcattgt tgagacgaaa gtggaagaag
     1801 aggttcaacg tatttcgcgg ggtagaggct aagcttatca ccgatacgcg agcgaacgtg
     1861 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     1921 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     1981 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     2041 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     2101 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     2161 cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
     2221 catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     2281 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     2341 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     2401 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2461 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2521 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     2581 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2641 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     3061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagcgg ccgccttcat ttaaatggcg
     3601 cgccttacgc cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg
     3661 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     3721 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     3781 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     3841 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     3901 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     3961 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     4021 tcaccgtctt tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg
     4081 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     4141 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     4201 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     4261 attttagctt ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat
     4321 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     4381 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     4441 aagtgatctt ccgtcacagg tagagatct
//
