LOCUS       sequence-258465-       12697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6A01
FEATURES             Location/Qualifiers
     source          1..12697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             826..1617
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1769..1844
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2458..2802
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2985..3050
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3057..3077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3102..3125,3126..3128,3129..5615,5616..5618,
                     5619..7202)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7115..7123
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7203..7250
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7269..7289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7305..7325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7332..7481
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7488..7508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7515..7541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      7594..7846
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7963..8308
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8381..9406
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    9447..9621
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9771..9795
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10043..10066)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(10074..10101)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(10193..10279)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      10448..10883
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     rep_origin      complement(10998..11586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11078..11097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11676..12485)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12399..12418
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12477..12496
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
       61 gaaggcggga aacgacaatc tgatccaagc tcaagctaag ctcacgtgac ggaattaagc
      121 ttcgacttgc cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt
      181 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt
      241 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga
      301 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata
      361 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata
      421 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa
      481 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
      541 gtgattggga gaccaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      601 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      661 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
      721 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      781 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
      841 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
      901 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
      961 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1021 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1081 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1141 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1201 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1261 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1321 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1381 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1441 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1501 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1561 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     1621 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     1681 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     1741 tttaagcgtg cataataagc cggtctcggt tttagagcta gaaatagcaa gttaaaataa
     1801 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ttgcaaaatt
     1861 ttccagatcg atttcttctt cctctgttct tcggcgttca atttctgggg ttttctcttc
     1921 gttttctgta actgaaacct aaaatttgac ctaaaaaaaa tctcaaataa tatgattcag
     1981 tggttttgta cttttcagtt agttgagttt tgcagttccg atgagataaa ccaataagct
     2041 tgcatgcctg caggtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     2101 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     2161 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     2221 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     2281 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     2341 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga
     2401 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
     2461 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
     2521 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
     2581 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2641 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2701 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     2761 cccttcctct atataaggaa gttcatttca tttggagagg acctcgacct caacacaaca
     2821 tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa
     2881 tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatactc
     2941 gagggggatc cccaatactt gtatggccgc ggccgctcta gatggattac aaggaccacg
     3001 acggggatta caaggaccac gacattgatt acaaggatga tgatgacaag atggctccga
     3061 agaagaagag gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg
     3121 gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc
     3181 cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca
     3241 ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg
     3301 cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca
     3361 acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg
     3421 aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct
     3481 accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata
     3541 aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt
     3601 tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc
     3661 tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg
     3721 cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc
     3781 agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc
     3841 tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca
     3901 aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg
     3961 acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg
     4021 tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc
     4081 accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca
     4141 aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct
     4201 ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg
     4261 agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg
     4321 gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg
     4381 acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga
     4441 tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca
     4501 agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg
     4561 ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc
     4621 tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga
     4681 agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca
     4741 tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact
     4801 acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca
     4861 acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg
     4921 acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg
     4981 accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca
     5041 tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca
     5101 acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct
     5161 tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata
     5221 tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg
     5281 ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg
     5341 tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc
     5401 agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca
     5461 ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga
     5521 atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc
     5581 tggatattaa caggctcagc gattacgacg tcgatgccat cgttccacag tcattcctga
     5641 aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg
     5701 ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga
     5761 atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc
     5821 tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca
     5881 ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca
     5941 agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga
     6001 aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt
     6061 acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt
     6121 tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg
     6181 agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga
     6241 cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg
     6301 agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct
     6361 ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg
     6421 agtcaattct gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc
     6481 cgaagaagta cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga
     6541 aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga
     6601 ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca
     6661 aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga
     6721 acggcaggaa gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc
     6781 tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca
     6841 gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg
     6901 agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg
     6961 ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga
     7021 atatcattca tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg
     7081 acacaactat cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca
     7141 tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg
     7201 acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag tccgggtcat
     7261 caggcagccc taagaagaag aggaaggtcg gcagcagcgg cagccccaag aagaagagga
     7321 aggtcgggtc ggacgccctc gacgatttcg acctcgatat gctgggctcc gacgccctgg
     7381 acgacttcga cctcgatatg ctcgggagcg acgcgctcga cgactttgac ctcgatatgc
     7441 tcgggtcgga cgctctggac gattttgacc tcgatatgct ggggtcgcct aagaagaagc
     7501 ggaaggttgg ctcataccca tacgatgtgc ccgactacgc ctcctgagct caggcctcca
     7561 tacaagtatt ggggatccac tacgaatttc cccgatcgtt caaacatttg gcaataaagt
     7621 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     7681 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     7741 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     7801 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attcgaggcg
     7861 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
     7921 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     7981 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     8041 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     8101 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     8161 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     8221 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     8281 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
     8341 atctctctcg agctttcgca gatccggggg gcaatgagat atgaaaaagc ctgaactcac
     8401 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     8461 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     8521 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     8581 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta gcgagagcct
     8641 gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc ctgaaaccga
     8701 actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg cggccgatct
     8761 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     8821 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     8881 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     8941 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     9001 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     9061 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     9121 ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct
     9181 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     9241 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     9301 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     9361 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta gatgccgacc
     9421 gggatctgtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat
     9481 aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag
     9541 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     9601 tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta cattaaaaac
     9661 gtccgcaatg tgttattaag ttgtcactag tcaggttaac tcaattcggc gttaattcag
     9721 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     9781 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     9841 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     9901 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     9961 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    10021 ctgcctgtga tcacttaagt aactaactaa caggaagagt ttgtagaaac gcaaaaaggc
    10081 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
    10141 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
    10201 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
    10261 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcttagta gttagacgtc
    10321 cccgagatcc atgctagacc atgaatccag aagcccgaga ggttgccgcc tttcgggctt
    10381 tttctttttc aaaaaaaaaa atttataaaa cgatctgttg cggccggccg ccgggttgtg
    10441 ggcaaaggcg ctcgacggtg ggcaaccgct tgcggttgtc cacgggcgga gccggtgcgc
    10501 gtagcgcatt gtccacaagc caagggcgac caataattga tatatatatt cataattgaa
    10561 aagctaattg aacatactac ttgctgtaac tacttgccgg agcgaggggt gtttgcaagc
    10621 tgttgatctg aaagggctat tagcgttctc acgtgccttt ttgattagcg atttcacgtg
    10681 accttattag cgatttcacg tactccgatt agcgatttca cgtaccctga ttagcgattt
    10741 cacgtggata gtttttggag cgggccggaa agccccgtga atcaaggctt tgcggggcat
    10801 tagcggtttc acgtggataa ctaccctcta tccacaggct tccggggata aaaaagcccg
    10861 ctcgacggcg ggctgttgga tggggatcta gcggtaatac ggttatccac agaatcaggg
    10921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    11041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    11461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cggggcccaa
    11641 tctgaataat gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
    11701 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
    11761 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
    11821 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
    11881 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
    11941 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
    12001 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
    12061 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag ggacactgcc
    12121 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
    12181 tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
    12241 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
    12301 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
    12361 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
    12421 tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg
    12481 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
    12541 atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgcagt
    12601 ttgatcccga ggggaaccct gtggttgaca tgcacataca aatggacgaa cggataaacc
    12661 ttttcacgcc cttttaaata tccgttattc taataaa
//
