LOCUS       sequence-258476-       10403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% 
            glucose, induced with 0.3% xylose; for homologous recombination at 
            the xylose locus in Caulobacter crescentus; tetracycline resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT
  TITLE     A CRISPR Interference System for Efficient and Rapid Gene Knockdown 
            in Caulobacter crescentus.
  JOURNAL   mBio. 2020 Jan 14;11(1):e02415-19. doi: 10.1128/mBio.02415-19.
  PUBMED    31937638
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/mBio"; 
            journalName: "mBio"; date: "2020-01-14- 14"; volume: "11"; issue: 
            "1"; pages: "e02415-19"
COMMENT     Sequence Label: pXGFPC-5 Pxyl-dcas9 (Spy)
FEATURES             Location/Qualifiers
     source          1..10403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             join(2869..2895,2896..2898,2899..5385,5386..5388,
                     5389..6975)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7127..7143)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7154..7170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7368..7956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7448..7467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8062..8078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8175..9374)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLSFVRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             9480..10130
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHSTSVPRADDDWRSFLTGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 cgctaccgga ctcagatcca gatcgaaatg taccggctga actggtggaa gcacgttgag
      541 cctcgtcaag gactcatgaa gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag
      601 gtgtcaaaac accgtctgtt catttggcta gacgctagag ttcttccgga tcacaagctc
      661 caggtcgtta cactcgatga cgactgctca ttcggagttt tacactcaag gttccacgag
      721 gtgtgggctc ttgcagcggg gtcgtggcac ggctcaggca atgatccgcg ctacaccatc
      781 tccaccacct tcgagacctt ccccttcccg gaaggcctga cgcccaacat agccgccgtc
      841 gactatgagg gcgacccgcg cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg
      901 ctgcgggaag cctggctcaa tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc
      961 ggctatcccg accgcgtgct gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc
     1021 accctgacca acctctacaa ccagcgcccg gcctggctgg acatggccca ccagcgcctg
     1081 gacgccgccg tcgccgccgc ctacggctgg cccgacggcc tgaccgacga cgagatcctg
     1141 gagcgcctgt tcgccctcaa ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc
     1201 caaaaccccg acctccccgg cgaaggccgg ggtccagtta catccggaac gcttggagtg
     1261 ggcccgccta cggcctcgac caacccgcag tcggcaaggg gttcgatctg gaccccggca
     1321 ttcgccgggg aggtcggcag agaggtcaga accgcgtctt gcagccctgc ttcagatgaa
     1381 ccccgcgcag gaagccgcgg cgggccaggc cggcgtcgac ggcggcgttc atctggcggt
     1441 cggcgggggc agcgccggtg acttcgcgca ccaggccccg gaacgggatg atgctgttga
     1501 tcagggtctt gccaccggcc tcggccagct tgccggctcg gttttcggcg tactcggcgc
     1561 cgaccttgta gtcgggcccc agcgccgcgt tcagcgcctt cacctcattg gccagggcct
     1621 ggcaggactt cagacccgcg aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg
     1681 gcacctcgcg cttggacagg ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct
     1741 tcttgcccgc ctcgccccag cggccggacg gttcctcggg cttggccggc ggcttgacct
     1801 gggcgtgggc gctggaggcc agcagcacgg cggcggctcc ggccatgatc agcagcttgt
     1861 tcggcatcaa aagcgccctc ccgacaaaac acgccacggg aacggcaagc ctcgccgccg
     1921 gttcccgccc gcaagtccct cggcacgaac tctaaggcct gcgcacgatc catcgccacc
     1981 acaaagcgtg tcatcccggc cgaagcgcag cggagagccg ggacccaggg ccacaagcac
     2041 ggcgcttcgt caccccctgg gtcccggata gcgctccgcg cttccgggat gacacgggtt
     2101 taagcacttg ggtgagaagc cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc
     2161 gtcgaaggcg tcgtcgccag gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag
     2221 cgatgaccag atgaaggcca tctgctccat gatcgtcggg acgctggccg gatcgaagcc
     2281 ggggtagagg tcttccacca gccacaggcc cgtgccggga tcgaaggcga agcggcagcc
     2341 gatcaggcgg aactgggcgc gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc
     2401 gtccaggtcg tcgtggtcca gcaccaggca gcggatctgc agccagccgt ggtcgggcag
     2461 caggtagaag gcgccctcgt cctgatcctc gcccgaaacc tccagccccc ggtcgatggc
     2521 ttcgacgaca tagccggccg cgcggcaggt gtcggtgagc gcggccagca gggcggcttc
     2581 ctggtcaggg gtcaggtcgg tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt
     2641 ctagcatgga ccgcccgcgc ccgtgaggcc gaggatttcg cgctggtcag acaacctact
     2701 tgccgtcccc acatgttagc gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg
     2761 gcgcttcaga cgctcgagtt ttggggagac gaccatatgc ctgcaggcgc cttaattaat
     2821 atgcatggta ccttaagatc tcgagctcct gactcaggag gtatcgatat ggataagaaa
     2881 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
     2941 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
     3001 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
     3061 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     3121 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     3181 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     3241 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     3301 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     3361 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     3421 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     3481 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     3541 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     3601 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     3661 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     3721 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     3781 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     3841 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     3901 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     3961 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     4021 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     4081 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     4141 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     4201 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     4261 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     4321 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     4381 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     4441 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     4501 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     4561 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     4621 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     4681 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     4741 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     4801 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     4861 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     4921 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     4981 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     5041 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     5101 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     5161 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     5221 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     5281 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     5341 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
     5401 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     5461 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     5521 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     5581 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     5641 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     5701 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     5761 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     5821 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     5881 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     5941 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     6001 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     6061 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     6121 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga
     6181 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     6241 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     6301 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     6361 gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     6421 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     6481 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     6541 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     6601 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     6661 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     6721 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     6781 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     6841 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     6901 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     6961 ctaggaggtg actaaatact ctggccattt ttctttaccg cctttgagtg agctgatacc
     7021 gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gctgattagc taagcagaag
     7081 gccatcctga cggatgcctt tttcgcttgc tagctgcagc ccgggggatc cactagttct
     7141 agagcggcca ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     7201 cccaacttaa tcgccttgca gcacatcccc ctttcgccag cggtatcagc tcactcaaag
     7261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat acgaggccct
     8041 ttcgtcttca agaattaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8101 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagca gatccgtccc
     8161 tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc cgctccaaag cccgcgacgc
     8221 agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca cccgttccac
     8281 gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg
     8341 ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc cacctgcctg
     8401 gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag gaccatgatc
     8461 gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca
     8521 atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg gccggtgatc
     8581 attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc
     8641 gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg
     8701 acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat gccccgggcc
     8761 caccgaacga agctgagcgg gttgagagcc tcccggcgta acggccggcg ttcgcctttg
     8821 tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa ggctgccgcg
     8881 gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac aggtcccgcg
     8941 accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca
     9001 tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt gatgccggcc
     9061 acgatccgcc cgatatagag aacccaaagg aaaggcgctg tcgccatgat ggcgtagtcg
     9121 acagtggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg atccgacagc
     9181 gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag cagaatgcca
     9241 tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc
     9301 ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt
     9361 atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga
     9421 ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca
     9481 tgacaaagtt gcagccgaat acagtgatcc gtgccgccct agacctgttg aacgaggtcg
     9541 gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc
     9601 tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg
     9661 cggagaatca tagcacttcg gtgccgagag ccgacgacga ctggcgctca tttctgactg
     9721 ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg
     9781 ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct
     9841 gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc agctacttca
     9901 ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag cgcggcggca
     9961 ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag
    10021 ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa
    10081 ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga tcaggaccgc
    10141 tgccggagcg caacccactc actacagcag agccatgtag acaacatccc ctcccccttt
    10201 ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct gccctagcgt
    10261 ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctcct gctgcggcgt
    10321 ccgctcgtat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
    10381 aacgtggctt tccccccccc ccc
//
