LOCUS       sequence-258489-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eGFP mRNA from the Drosophila Ubi-p63e promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Tatomer DC, Elrod ND, Liang D, Xiao MS, Jiang JZ, Jonathan M, Huang 
            KL, Wagner EJ, Cherry S, Wilusz JE
  TITLE     The Integrator complex cleaves nascent mRNAs to attenuate 
            transcription.
  JOURNAL   Genes Dev. 2019 Sep 17. pii: gad.330167.119. doi: 
            10.1101/gad.330167.119.
  PUBMED    31530651
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 17. pii: gad.330167.119. doi: 10.1101/gad.330167.119."
COMMENT     Sequence Label: Hy_pUbi-p63e eGFP SV40
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          14..79
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             209..229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    654..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(2819..2821,2822..2824,2825..3538)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2819..2821,2822..2824,2825..3535)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2864..2885)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3125..3144)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3472..3493
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3547..3668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4378..4395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4549..5137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4629..4648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5308..6099,6100..6168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5931..5950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6169..6273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6341..6359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7755..8034
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             8080..9105
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8080..9105
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
       61 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
      121 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
      181 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
      241 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
      301 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
      361 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
      421 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
      481 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
      541 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      601 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      781 tctggattaa ttaacttgtc gccggaacgc agcgacagag attccaatgt gtccgtatct
      841 ttcaggcttt tgcccttcag ttccagacga agcgactggc gattcgcgtg tggggtctgc
      901 ttcagggtct tgtgaattag ggcgcgcaga tcgccgatgg gcgtggcgcc ggagggcacc
      961 ttcaccttgc cgtacggctt gctgttcttc gcgttcaaaa tctccagctc cattttgctt
     1021 tcggtgcgct tgcaatcagt actgtccaaa atcgaaaatc gccgaaccgt agtgtgaccg
     1081 tgcggggctc tgcgaaaata aactttttta ggtatatggc cacacacggg gaaagcacag
     1141 tggattatat gttttaatat tataatatgc aggttttcat tacttatcca gatgtaagcc
     1201 cacttaaagc gatttaacaa ttatttgccg aaagagtata aacaaatttc acttaaaaat
     1261 ggattaagaa aagctagctt gtgtaagatt atgcgcagcg ttgccagata gctccattta
     1321 aaacacttca aaaacaataa gttttgaaaa tatatacata aatagcagtc gttgccgcaa
     1381 cgctcaacac atcacacttt taaaacaccc tttacctaca cagaattact ttttaaattt
     1441 ccagtcaagc tgcgagtttc aaaattatag ccggtagaga agacagtgct atttcaaaag
     1501 caaactaaca agggtcttaa attccaaaac accaatccta acaagccttg gacttttgta
     1561 agtttagatc aaaggtggca ttgcattcaa tgtcatggta agaagtaggt cgtctaggta
     1621 gaaatcctca ttcagccggt caagtcagta cgagaaaggt ctcaatttga aattgtctta
     1681 aaaatatttt attgttttgt actgtggtga gtttaaacga aaaacacaaa aaaaaagtga
     1741 tacacagaaa tcataaaaaa ttttaataca aggtattcgt acgtatcaaa aacatttcgg
     1801 cacaattttt tttctctgta ctaaagtgtt acgaacacta cggtattttt tagtgatttt
     1861 caacggacac cgaaggtata taaacagcgt tcgcgaacgg tcgccttcaa aaccaattga
     1921 catttgcagc agcaagtaca agtagaaagt aaagcgcaat cagcgaaaaa tttatactta
     1981 attgttggtg attaaagtac aattaaaaga acattctcga aagtcacaag aaacgtaagt
     2041 ttttaactcg ctgttaccaa ttagtaataa gagcaacaag acgttgagta atttcaagaa
     2101 aaactgcatt tcaaggtctt tgttcggcca ttttttttta ttcaacgctc tacgtaatta
     2161 caaaataaga aattggcagc cacgcatctt gttttcccaa tgaattggca tcaaaacgca
     2221 aacaaatcta taaataaaac ttgcgtgttg attttcgcca agatttattg gcaaattgtg
     2281 aaattcgcag tgacgcattt gaaaattcga gaaatcacga acgcactcga tcgagcattt
     2341 gtgtgcatgt tattagttag ttgtttagtt aattgaagta ttttaccaac gaaatccact
     2401 tatttttagc tgaaatagag taggttgctt aaacaaagcc acgtctgaaa atttcttatt
     2461 gcttgtagtt gtgacgtcac catatacaca caaaataatg tgtatgcatg catttcagct
     2521 gtgtatatat acatgcacac actcgcaaca cgaaaacgat gacgaagcaa cggaacaaag
     2581 gtttctcaac taccctttgt tccctgtttc ttcgctttcc tttgttccaa tattcgtaga
     2641 gggttaatag gggtttctca acaaagttgg cgtcgataaa taagtttccc atttttattc
     2701 cccagccagg aagttagttt caatagtttt gtaatttcaa cgaaactcat ttgatttcgt
     2761 actaattttc cacatctcta ttttcttccc gcagaataat ccatctagag atatcaccat
     2821 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2881 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2941 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3001 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     3061 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     3121 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3181 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     3241 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     3301 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     3361 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     3421 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     3481 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     3541 ggccgcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3661 gtatcttaac tagtggatcc actaggggcc gccgacgcga ggctggatgg ccttccccat
     3721 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     3781 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     3841 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     3901 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     3961 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     4021 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     4081 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
     4141 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     4201 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     4261 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     4321 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgctctt ccgcttcctc
     4381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4981 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     5641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     5941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     6361 ctttcgtctt caagaattca catttgtacg aatttttttt ttatcaaaag ttcgagtttt
     6421 tcaccaattt cctcatcaac cgagcaaggc aaacggcttt gaataatatg gtgttatata
     6481 tacatatatc aaatcgctgc tgactgcgtg attgatggcc ccaagattac atattatcga
     6541 atcaggattc agaaggagat caatgtcaaa tgcggacagg aacatgaaag acgcctgtta
     6601 tgcgcaatta aaaatttggg tttaattgct gtggaaactg ttgttggcgg catcttaagt
     6661 tcctgtttaa caacatcaac tacttatgta cgtagaagcg tttaagccat ttgcatacag
     6721 atgagaactg gcttttgtgc taatcagtca agatgactcc gatgatgatg actcattacc
     6781 tgaccagttt tcgctgcttt cttttcaaca actacttgta tatgtattgt atccaatagc
     6841 aatacattga atttccatgg tctagtcacg tattatcatt taattgacac caagtcgtgt
     6901 tattgttgag ctatcgagtt cagctcaaac atttcttatt cccatgaata agccggcaaa
     6961 aatatgcaat ctatgaaagt taatataagc aaaccttact ttgactcaat accaatgcac
     7021 tttgtgtcga taggttcacg caattgaggc gattattccg ataacccaag cgattgactg
     7081 ttcccgtttc gattccaatt gaaatttgga aatgtacaat agttttgcta tatgctgtca
     7141 agtacgctct tatcttctct gggttttctt cagagtttcg aaacgcttct tctttttttt
     7201 gttttttttt ttttggaatc tcgtattttg gaaggggctc ccctctggaa tttgttacac
     7261 tgtcgttatc attgcgaaca agcggcccga agctatcagc gactttaaca tttacaatgc
     7321 acttttttac gaccaattaa atgtacattt tcctttcttc gcccgttgat aagcgaacgc
     7381 gatgtggcgc aggcaatgtg ttgctcttgc gacacaaacg caatcaaaat ggattcaatt
     7441 tcgctttttc ccagtgaaac gaagaacgaa ccgaccatca tgatatgctc ctctgcatgt
     7501 tgcgtattga atcaatgaca atttcaatta agccgcccgt tcgtcatgcg ttttcgtgcg
     7561 cttcgaaatg ctgataacgc tgctgtcctc caactgcttt gcatgtggac acaattccat
     7621 ttatttaatt cttttatttg gatcggttaa attaaaaagc gccttgttac gcatttaacg
     7681 ttgtttccgg tgcgtggtgg tttcatgctt ctgggaacgg caaatgggtt taggattggg
     7741 aacccctcat catctgttgg aatatactat tcaacctaca aaagtaacgt taaacaacac
     7801 tactttatat ttgatatgaa tggccacacc ttttatgcca taaaacatat tgtaagagaa
     7861 taccactctt tttattcctt ctttccttct tgtacgtttt ttgctgtaag taggtcgtgg
     7921 tgctggtgtt gcagttgaaa taacttaaaa tataaatcat aaaactcaaa cataaacttg
     7981 actatttatt tatttattaa gaaaggaaat ataaattata aattacaaca ggttatggac
     8041 ctgcagccaa gcttggcgct cgtccggggg caatgagata tgaaaaagcc tgaactcacc
     8101 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     8161 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     8221 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     8281 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     8341 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     8401 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     8461 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     8521 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     8581 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     8641 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     8701 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     8761 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     8821 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     8881 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     8941 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     9001 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     9061 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     9121 aacaagagct gatttcgaga acgcctcagc cagcaactcg cgcgagccta gcaaggcaaa
     9181 tgcgagagaa cggccttacg cttggtggca cagttctcgt ccacagttcg ctaagctcgc
     9241 tcggctgggt cgcgggaggg ccggtcgcag tgattcaggc ccttctggat tgtgttggtc
     9301 cccagggcac gattgtcatg cccacgcact cgggtgatct gactgatccc gcagattgga
     9361 gatcgccgcc cgtgcctgcc gattgggtgc agatctttgt gaaggaacct tacttctgtg
     9421 gtgtgacata attggacaaa ctacctacag agat
//
