LOCUS       sequence-258522-       10943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH66
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2029..2049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2062..2127
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2152..6255
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2152..6255
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      6346..6598
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6646..6670
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6826..7974)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8310..8898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8390..8409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9069..9860,9861..9929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9692..9711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9930..10034)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10121..10752
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10844..10868
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggcacc gaagaagaag
     2041 cgcaaagtgg cccacgtgat ggactataag gaccacgatg gcgactacaa ggaccatgac
     2101 attgactata aggatgacga cgataagaac gcccagagcg gcgccagcgt catggacaag
     2161 aagtacagca tcggcctgga catcgggacc aacagcgtgg ggtgggcggt tatcacggac
     2221 gagtacaagg tgccaagcaa gaagttcaag gtgctgggca acaccgaccg ccactccatc
     2281 aagaagaacc tcatcggagc cctcctcttc gacagcggcg aaacggctga ggctaccagg
     2341 ctcaagagga ccgctaggcg caggtacacc cgcaggaaga acaggatctg ctacctccaa
     2401 gaaatcttca gcaacgagat ggcgaaggtg gacgactcct tcttccaccg cctggaggag
     2461 agcttcctcg tcgaggaaga taagaagcac gagaggcacc caatcttcgg caacatcgtg
     2521 gacgaggtcg cctaccacga gaagtaccca accatctacc acctgaggaa gaagctcgtg
     2581 gacagcaccg acaaggccga cctccgcctg atctacctcg ccctggccca catgattaag
     2641 ttcaggggcc acttcctgat cgagggcgac ctcaacccag acaactccga cgtggacaag
     2701 ctgttcatcc aactcgtcca gacctacaac caactcttcg aggagaaccc aatcaacgct
     2761 tccggcgtgg acgctaaggc tatcctgagc gccaggctct ccaagtcccg caggctggag
     2821 aacctgatcg cccagctccc aggcgagaag aagaacggcc tgttcggcaa cctcatcgct
     2881 ctctccctgg gcctcacccc aaacttcaag agcaacttcg acctcgctga ggacgccaag
     2941 ctgcaactca gcaaggacac ctacgacgac gacctcgaca acctcctggc ccagatcggc
     3001 gaccaatacg ccgacctgtt cctcgccgcc aagaacctgt ccgacgccat cctcctgagc
     3061 gacatcctcc gcgtgaacac cgagatcacc aaggccccac tctccgccag catgatcaaa
     3121 cgctacgacg agcaccacca ggacctgacc ctcctgaagg ccctggtcag gcaacagctc
     3181 ccagagaagt acaaggaaat cttcttcgac cagtccaaga acggctacgc tggctacatc
     3241 gacggcggag cgagccaaga ggagttctac aagttcatca agccaatcct ggagaagatg
     3301 gacggcaccg aggagctgct ggtgaagctc aacagggagg acctcctgag gaagcagcgc
     3361 accttcgaca acggctccat cccacaccaa atccacctcg gcgagctgca cgctatcctc
     3421 cgcaggcaag aggacttcta cccattcctc aaggacaaca gggagaagat cgagaagatc
     3481 ctgaccttcc gcatcccata ctacgtgggg ccactcgcca ggggcaactc ccgcttcgct
     3541 tggatgaccc gcaagagcga ggaaacgatc accccgtgga acttcgagga agtggtggac
     3601 aagggcgctt ccgctcagag cttcatcgag aggatgacca acttcgacaa gaacctgcca
     3661 aacgagaagg tgctcccaaa gcacagcctc ctgtacgaat acttcaccgt ctacaacgag
     3721 ctgaccaagg tgaagtatgt gaccgagggc atgaggaaac cagccttcct gtccggcgag
     3781 cagaagaagg ccatcgtgga cctcctgttc aagaccaaca ggaaggtgac cgtcaagcaa
     3841 ctcaaggaag attacttcaa gaagatcgag tgcttcgact ccgtggagat cagcggcgtc
     3901 gaggacaggt tcaacgccag cctcggcacc taccacgacc tcctgaagat catcaaggac
     3961 aaggacttcc tggacaacga ggagaacgag gacatcctgg aggacatcgt gctgaccctc
     4021 accctgttcg aggacaggga gatgatcgag gagcgcctca agacctacgc ccacctcttc
     4081 gacgacaaag ttatgaagca actgaagcgc aggcgctaca ccggctgggg caggctgtcc
     4141 cgcaagctca tcaacggcat ccgcgacaag cagtccggca agaccatcct cgacttcctg
     4201 aagagcgacg gcttcgccaa caggaacttc atgcaactga tccacgacga ctccctcacc
     4261 ttcaaggaag atattcagaa ggctcaagtc tccggccagg gcgacagcct gcacgagcac
     4321 atcgctaacc tcgctggctc cccagccatc aagaagggca tcctgcaaac cgtgaaagtc
     4381 gtggacgagc tggtgaaggt catgggcagg cacaagccag agaacatcgt catcgagatg
     4441 gcccgcgaga accaaaccac ccagaagggc caaaagaaca gcagggagcg catgaagcgc
     4501 atcgaggaag gcatcaagga gctgggctcc caaatcctca aggagcaccc agtcgagaac
     4561 acccaactcc agaacgagaa gctctacctg tactacctcc agaacggcag ggatatgtat
     4621 gtggaccaag agctggacat caaccgcctc agcgactacg acgtggacca catcgtccca
     4681 cagtccttcc tgaaggacga cagcatcgac aacaaggtgc tcaccaggtc cgacaagaac
     4741 cggggcaagt ccgacaacgt cccaagcgag gaagtggtca agaagatgaa gaactactgg
     4801 aggcagctcc tgaacgccaa gctcatcacc caaaggaagt tcgacaacct caccaaggct
     4861 gagcgcggcg gcctcagcga gctggacaag gcgggcttca tcaagaggca gctcgtggaa
     4921 acccgccaaa tcaccaagca cgtcgcccaa atcctcgact cccgcatgaa caccaagtac
     4981 gacgagaacg acaagctgat tagggaggtg aaggtcatca ccctgaagtc caagctcgtg
     5041 agcgacttca ggaaggactt ccagttctac aaggtccgcg agatcaacaa ctaccaccac
     5101 gcccacgacg cttacctcaa cgctgtggtg ggcaccgccc tcatcaagaa gtacccaaag
     5161 ctggagtccg agttcgtgta cggcgactac aaggtgtacg acgttcgcaa gatgatcgcc
     5221 aagagcgagc aagagatcgg caaggccacc gccaaatact tcttctactc caacattatg
     5281 aacttcttca agaccgagat caccctggct aacggcgaga tcaggaagcg cccactcatc
     5341 gaaacgaacg gcgagactgg cgagatcgtg tgggacaagg gcagggactt cgccaccgtc
     5401 cgcaaggtcc tctccatgcc acaggtgaac atcgtcaaga aaaccgaggt ccagaccggc
     5461 ggcttctcca aggagagcat cctgccaaag aggaactccg acaagctcat cgcccgcaag
     5521 aaggactggg acccaaagaa gtacggcgga ttcgactccc caaccgtggc ctacagcgtc
     5581 ctggtggtcg ccaaggtgga gaagggcaag tccaagaagc tcaagagcgt caaggagctg
     5641 ctgggcatca ccatcatgga gaggagcagc ttcgagaaga acccaatcga cttcctggag
     5701 gccaagggct acaaggaagt gaagaaggac ctgattatca agctcccaaa gtattccctc
     5761 ttcgagctgg agaacggcag gaagcgcatg ttggcttccg ctggcgagct gcaaaagggc
     5821 aacgagctgg ccctgccatc caagtatgtg aacttcctct acctggcctc ccactacgag
     5881 aagctcaagg gcagcccaga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag
     5941 cactacctcg acgagatcat cgagcaaatc tccgagttct ccaagcgcgt gatcctcgcc
     6001 gacgccaacc tggacaaggt cctcagcgcc tacaacaagc acagggacaa gccaatccgc
     6061 gagcaggccg agaacatcat ccacctcttc accctgacca acctcggcgc tccagcggcc
     6121 ttcaaatact tcgacaccac catcgacagg aaacgctaca cctccaccaa ggaagtgctc
     6181 gacgccaccc tcatccatca aagcatcacc gggctctacg aaacgagaat cgacctgtca
     6241 caactgggcg gcgacagttc tctaaacgcc accagcggga agcgcgtgcc taaggaggcc
     6301 ggagccggga ccgacaagaa gaagaaaagt taagcttgtc aagcagatcg ttcaaacatt
     6361 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     6421 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     6481 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     6541 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
     6601 cgctactatt gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg
     6661 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     6721 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca
     6781 ggcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
     6841 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
     6901 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
     6961 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
     7021 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
     7081 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
     7141 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
     7201 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
     7261 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
     7321 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
     7381 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
     7441 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
     7501 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
     7561 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     7621 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     7681 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag
     7741 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     7801 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     7861 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     7921 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     7981 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
     8041 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
     8101 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
     8161 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
     8221 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg
     8281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9181 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     9241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9961 tatcaaggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10021 ataggggttc cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg
    10081 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    10141 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    10201 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    10261 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    10321 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    10381 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    10441 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    10501 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    10561 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    10621 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
    10681 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
    10741 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg
    10801 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    10861 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    10921 ggggtggatg cagtgggccc cac
//
