LOCUS       sequence-258525-       10916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH67
FEATURES             Location/Qualifiers
     source          1..10916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2029..2049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      2098..2107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2104..6207
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2104..6207
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6211..6237
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6217..6243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6256..6276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6283..6303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6319..6571
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6619..6643
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6799..7947)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8283..8871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8363..8382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9042..9833,9834..9902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9665..9684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9903..10007)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10094..10725
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10817..10841
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggggcc gaagaagaag
     2041 aggaaggtgg gtagggtgtg tcggattagc agccttcggt acaggggtcc tggcattgcc
     2101 accatggaca agaagtactc tatcgggttg gatataggaa cgaatagtgt gggttgggct
     2161 gtcatcactg acgagtacaa ggtgccatcg aagaaattta aagtgctggg caacaccgac
     2221 aggcactcca tcaagaagaa cctaataggt gcgttgctct ttgattccgg ggagacagcc
     2281 gaagcgaccc gcctcaagcg cacagcaaga aggcgctata cgcgtagaaa gaacaggatc
     2341 tgctacctac aagagatctt ttccaacgaa atggcgaaag tggatgactc cttcttccat
     2401 cggctcgagg aaagcttcct cgtagaagag gacaagaagc acgaacgcca tccgatattc
     2461 gggaacattg tcgacgaagt cgcataccac gagaagtatc ctaccatcta tcacctgagg
     2521 aagaagctcg tagatagcac tgacaaggct gatttgcgcc tcatctattt ggccctggcc
     2581 catatgatta agttccgtgg gcacttcctc atcgaaggcg accttaatcc ggacaattcc
     2641 gatgttgaca aactcttcat ccaactggtc cagacataca atcagctctt tgaagagaac
     2701 cccatcaacg cgtcaggagt tgatgcgaag gcaatcttga gtgctcggct gtcgaaaagc
     2761 cggaggctcg aaaatctgat agcccaactg ccaggcgaaa agaagaacgg attgtttggc
     2821 aaccttattg cccttagtct agggctcacc cctaacttca agtctaactt tgacctggcc
     2881 gaggatgcca agctccaact gtccaaagac acgtacgacg acgatctcga caatctgctt
     2941 gcccaaatcg gcgaccagta tgcggacctt ttccttgcag cgaagaactt gtcagatgca
     3001 atcctcttgt ccgatattct gcgcgttaac actgagatta ccaaggctcc tctttcggca
     3061 tccatgatca aacggtacga cgaacatcac caggatctca cgctcctcaa ggctctcgtc
     3121 agacaacagc tgccggagaa gtacaaagag atcttcttcg atcaatcaaa gaacgggtat
     3181 gctgggtaca tcgacggtgg cgcgtcacag gaggagttct acaagtttat caagcccatt
     3241 ctcgagaaga tggatggcac tgaagagctc ctggttaagc tcaatcgcga ggatctactc
     3301 cgcaagcagc gtaccttcga caatggaagc attccacacc aaatccatct gggcgagctt
     3361 cacgccatac ttcgtaggca agaggacttt tacccattcc tcaaagacaa ccgggagaaa
     3421 atcgagaaga tcctcacatt cagaatcccc tactatgtgg gcccattggc aaggggcaac
     3481 tctaggttcg cgtggatgac tagaaaatcg gaggaaacga ttaccccatg gaacttcgag
     3541 gaggtggtgg acaagggcgc ttctgcccaa agcttcatcg agaggatgac caacttcgac
     3601 aagaatctgc caaacgagaa ggtcctcccc aaacattccc tactgtacga gtacttcacc
     3661 gtctacaacg agctgaccaa ggtcaagtac gttactgagg gtatgaggaa accggccttt
     3721 ctgtcaggag agcagaagaa ggccatagtg gatctccttt tcaagaccaa tcgcaaggtc
     3781 acagtcaagc agctaaagga ggactacttt aagaaaatcg agtgctttga ctctgtggag
     3841 ataagcggcg ttgaggatcg cttcaacgcc tcgctgggaa cgtaccacga cttgctcaag
     3901 atcattaagg ataaagactt cctcgataac gaagagaacg aggacatctt ggaggacatc
     3961 gttctgacgc ttactctctt cgaggaccgc gagatgatcg aagagaggct caagacgtat
     4021 gcgcacctct ttgacgacaa agtgatgaag cagctgaagc gcaggcgcta cacaggttgg
     4081 ggcaggctga gcaggaagct gatcaatggc attcgcgata agcagtcggg caagaccatc
     4141 ctcgacttcc tcaagtccga tgggttcgca aacaggaact ttatgcagct aatccacgat
     4201 gactccttga ccttcaagga ggacattcag aaggctcagg tttcaggcca aggcgattcg
     4261 ctccacgagc atatcgcgaa tttggccgga tctccagcca tcaagaaggg tatacttcaa
     4321 acagtgaagg ttgtcgatga gctggtcaaa gtgatgggta gacataagcc cgagaatatc
     4381 gtgattgaga tggctcggga gaaccaaacc acacagaagg ggcagaagaa ctccagggaa
     4441 aggatgaaga gaatcgaaga aggcatcaag gagctcggat cacaaattct caaggagcat
     4501 ccggtcgaaa acacccaact gcaaaacgag aagctctacc tctactacct ccaaaacgga
     4561 cgcgatatgt acgtggacca ggagctcgac atcaatcgcc tatctgacta cgatgtcgat
     4621 catatagtac cacaaagctt cctcaaagac gactctatcg acaacaaggt gctgaccagg
     4681 tccgacaaga accgcgggaa gtcagacaac gtgccttctg aggaagtcgt caagaagatg
     4741 aagaactact ggaggcaact cctcaatgca aagctcatta cacaaaggaa gttcgacaac
     4801 cttaccaagg cggagagagg cggcctcagt gagctagata aggccggatt tatcaagcgc
     4861 caactggttg agacacggca aatcaccaag cacgttgcac agatcctgga ctcacgcatg
     4921 aacacaaaat acgatgagaa tgacaagcta atccgggaag tcaaagttat tactctgaaa
     4981 tctaaactgg tgtccgactt tcggaaggac ttccagttct ataaggtccg tgagatcaac
     5041 aactaccatc acgcccatga tgcctatctc aatgctgtgg ttggcactgc actcatcaag
     5101 aaatatccca agcttgagtc ggagttcgtc tacggtgact acaaggttta tgacgtaagg
     5161 aagatgattg cgaagagtga gcaggaaatc ggcaaggcca ccgccaagta cttcttttac
     5221 agcaacataa tgaacttctt caagactgag atcaccctgg ccaatggaga gatcagaaaa
     5281 cgtccgctga tagagactaa cggagaaact ggtgagatag tgtgggacaa ggggagagac
     5341 tttgctactg tccggaaggt gctaagcatg ccacaagtca acatcgtcaa gaaaacggaa
     5401 gtccaaaccg gcggcttctc gaaagagtca atccttccga agcgcaatag cgataagctt
     5461 atagcccgta agaaagactg ggatccaaag aagtacggtg gctttgacag tcctacagtg
     5521 gcgtactccg tgctggttgt tgccaaggtg gaaaagggga agtcgaagaa gctgaagagc
     5581 gtaaaggagt tgctcgggat taccattatg gagcgttcca gctttgagaa gaatcccatt
     5641 gatttcttgg aggcgaaagg atacaaggaa gtcaagaaag atctcatcat aaagttgccg
     5701 aaatacagct tgttcgagct ggagaatggg agaaagagga tgcttgccag cgcaggcgag
     5761 ttgcaaaagg ggaacgagct ggcactccca tccaagtacg tcaacttcct gtatctcgcc
     5821 tcacactatg agaaacttaa gggttcccca gaggacaatg agcaaaagca gctgtttgtg
     5881 gagcaacaca agcactacct cgatgagatc atcgaacaga tcagtgagtt ctcgaagagg
     5941 gtgattcttg ctgacgctaa ccttgacaag gtgttgtccg cctacaacaa gcatcgggac
     6001 aaacccatcc gggagcaggc agaaaacatc atacacttgt tcacgttgac gaaccttggc
     6061 gctcctgcgg cgttcaaata ttttgacacg acgattgata ggaagcgcta taccagcacc
     6121 aaagaagtac tcgatgccac cctaattcac cagtctatta caggcctgta cgaaacgagg
     6181 atcgacttgt cgcaactagg aggagatgcc tatccgtatg acgtgccaga ttacgcctcc
     6241 cttgggtctg gttcccctaa gaagaagcgg aaagtggagg atcccaagaa gaagcgcaaa
     6301 gtctaagctt gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     6361 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     6421 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     6481 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     6541 tcgcgcgcgg tgtcatctat gttactagat cgacgctact attgtcttct gcacgaagtg
     6601 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     6661 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     6721 atgtgcatgc caaccacagg gttccccaga tcaggcgctg gctgctgaac ccccagccgg
     6781 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     6841 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     6901 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     6961 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     7021 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     7081 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     7141 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     7201 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     7261 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     7321 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     7381 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     7441 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     7501 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     7561 ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag
     7621 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     7681 cgacttcgcc aaacctgccg cctcctgttc aagacgacgc gaacgctcca cggcggccga
     7741 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
     7801 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
     7861 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
     7921 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
     7981 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
     8041 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
     8101 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
     8161 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
     8221 ggcatcgttg cgccacatct aggatctgcc aggaaccgta aaaaggccgc gttgctggcg
     8281 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8341 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8401 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8461 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8521 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8581 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8641 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8701 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8761 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8821 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8881 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8941 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9001 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9061 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9181 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9301 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9901 atactcttcc tttttcaata ttattgaagc atttatcaag gttattgtct catgagcgga
     9961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac gaattggcca
    10021 gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg
    10081 ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg
    10141 cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta
    10201 aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg
    10261 ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca
    10321 gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc
    10381 aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc
    10441 gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga
    10501 aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca
    10561 acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg
    10621 cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag
    10681 acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcgcaaa ggagatcctg
    10741 atctgactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    10801 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    10861 ttaataacac attgcggacg tttttaatgt actggggtgg atgcagtggg ccccac
//
