LOCUS       sequence-25855-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 4r-DsRed-3r
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..152)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(216..239)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     631..651
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta
       61 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      121 cgtttctcgt tcaactttat tatacaaagt tgtctacagg aacaggtggt ggcggccctc
      181 ggtgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      241 ggcgtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt
      301 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtggg tctcgccctt
      361 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tcgtcttctt
      421 ctgcatcacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa
      481 gcagccgtcc tgcagggagg agtcctgggt cacggtcgcc acgccgccgt cctcgaagtt
      541 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
      601 ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtncnn
      661 nnnnnaggnn gnnggcgggc cccccttggn ccnntnnact ttccanggtg ntngnggccc
      721 ctcaaggggg ggcnnccttc nc
//
