LOCUS       sequence-25856-e         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEFS 4r-3r
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..135)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    396..519
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag ctctgttttt
       61 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt
      121 ttctatacaa agttgctgag ctcccgggct cgagtaccca ggtgtgacaa tagttgttat
      181 ttcagttctc tcttactttt agtgtanctg taatatagtt tggttatgag tgaacttgtt
      241 tgatgatttg gttgaccagc ttgntgcaat tggaacatat gaacttgtta tattcacacc
      301 aagacatggc atatgacttg tccgcacagc tgtctttagc gtactcccgt ataggttatt
      361 ttgtaccata tggtaaaggt gtgggcactc tagaacaact ttgtataata aagttgaacg
      421 agaaacgtan aatgatatna atatcaatat attaaattag attttgcata aaaaacagac
      481 tacatactgc tgtaaaccac aacatatgca gtcacgatga atcaactact tggatggtag
      541 t
//
