LOCUS       sequence-25858-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 3-6HTnos-2
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..95
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      115..367
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(366..465)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(519..535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(519..535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(637..656)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtataa tattgcgcac caccaccacc accactaaga gctcgaattt ccccgatcgt
      121 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
      181 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
      241 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
      301 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
      361 ctgataccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      421 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccgtc cggntgcagg
      481 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
      541 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      601 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
      661 aacgggaa
//
