LOCUS       sequence-25859-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 3-G6HTnos-2
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(112..364)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(384..401)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(442..462)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgcgcgctg gaggcaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtatcagta
      121 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
      181 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      241 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      301 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      361 gatcggggaa attcgagttc ttagtggtgg tggtggtggt gtttgtatag ttctaccatg
      421 ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc tctcttttcg
      481 ttgggatctt tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg taaaaggaca
      541 gggccatcgc caattggagt attttgttga taatgatcag cgagttgcac gccgccgtct
      601 tcgatgttgt ggcgggtctt gaagttggct ttgatgccgt tcttttgctt gtcggcccat
      661 gatgtatacg ttgtgggagt tgtagttgta ttccaacttg tggccgagga tgtttccgtc
      721 ctccttgaaa tcgattccct taagctcgat cctggtg
//
