LOCUS       sequence-258608-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPlk_N + pSiMPlk_C
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatgctga aaaaaatcct gaaaatcgaa gagctggatg aacgtgaact gatcgatatt
       61 gaggtgtccg gtaaccacct gttttacgct aacgatattc tgacccacaa ctcttcttct
      121 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      181 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      241 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      301 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
      361 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
      421 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
      481 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      541 aataaattgc agtttcattt gatgctcgat gagtttttct aa
//
