LOCUS       sequence-25861-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 3-CFP-2
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(98..124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    137..202
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(245..266)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     594..613
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtaagc gtaatctgga acatcgtatg
      121 ggtatctaga tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt
      181 gagctcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
      241 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      301 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      361 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      421 tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctga
      481 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      541 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      601 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      661 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gcctgaagca ctgcacgccc
      721 c
//
