LOCUS       sequence-258621-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPla_N + pSiMPla_C
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatgctga aaaaaatcct gaaaatcgaa gagctggatg aacgtgaact gatcgatatt
       61 gaggtgtccg gtaaccacct gttttacgct aacgatattc tgacccacaa ctcttcttct
      121 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      181 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      241 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      301 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tacagcaatg
      361 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      421 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      481 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      541 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      601 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      661 cattggtaa
//
