LOCUS       sequence-258622-         381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPlh_N + pSiMPlh_C
FEATURES             Location/Qualifiers
     source          1..381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatgctga aaaaaatcct gaaaatcgaa gagctggatg aacgtgaact gatcgatatt
       61 gaggtgtccg gtaaccacct gttttacgct aacgatattc tgacccacaa ctcttcttct
      121 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
      181 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
      241 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
      301 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
      361 cgtccgaggg caaaggaata a
//
