LOCUS       sequence-258629-       10541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, 
            Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S
  TITLE     Optimisation of bioluminescent reporters for use with mycobacteria.
  JOURNAL   PLoS One. 2010 May 24;5(5):e10777.
  PUBMED    20520722
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-05-24"; volume: "5(5)"; pages: "e10777"
COMMENT     Sequence Label: pMV306hsp+LuxG13
FEATURES             Location/Qualifiers
     source          1..10541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      109..697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     598..617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1367..2449
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGVTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             2464..3447
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAAADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             3934..5376
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             5389..6312
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVALRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             6337..7449
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQAELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      7510..7556
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(9493..9512)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9504..10319
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9577..9596)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
       61 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      241 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
      541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      841 gaccgagcgc aacgcgtgcg gccgctctag acggtgacca caacgcgccc gctttgatcg
      901 gggacgtctg cggccgacca tttacgggtc ttgttgtcgt tggcggtcat gggccgaaca
      961 tactcacccg gatcggaggg ccgaggacaa ggtcgaacga ggggcatgac ccggtgcggg
     1021 gcttcttgca ctcggcatag gcgagtgcta agaataacgt tggcactcgc gaccggtgag
     1081 tgctaggtcg ggacggtgag gccaggcccg tcgtcgcagc gagtggcagc gaggacaact
     1141 tgagccgtcc gtcgcgggca ctgcgcccgg ccagcgtaag tagcggggtt gccgtcaccc
     1201 ggtgaccccc gtttcatccc cgatccggag gaatcacttc gcaatggcca agacaattgc
     1261 ggatcccccg ggctgcagga attcgaatgg ccatgggacg tcgacctgag gtaattataa
     1321 cccgggccct atatatggat ccaattcgtc gacaggagga ctctctatga aatttggaaa
     1381 ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt
     1441 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca
     1501 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc
     1561 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg
     1621 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat
     1681 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg
     1741 cgccttagcg gaatgctggt acgggctgat aaagaatggc gtgacagagg gatatatgga
     1801 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag
     1861 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca
     1921 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact
     1981 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg
     2041 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa
     2101 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga
     2161 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca
     2221 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca
     2281 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg
     2341 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc
     2401 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa
     2461 gaaatgaaat ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa
     2521 agtatagttc gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt
     2581 ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct
     2641 ggttttctgc tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca
     2701 actcatcatc ctgtccgcat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg
     2761 agatttattt tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc
     2821 ccggttgaat atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta
     2881 acaacaggct attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat
     2941 ccccatgctt atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt
     3001 gttgagtggg cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat
     3061 gttagatatg aatatgctga aagatataaa gccgctgcgg ataaatatga cgttgaccta
     3121 tcagagatag accatcagtt aatgatatta gttaactata acgaagatag taataaagct
     3181 aaacaagaga cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat
     3241 ttcgaaaata aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt
     3301 ataactgcgg ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt
     3361 gaaccaatga atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt
     3421 aagaagtacc acatgaaata tacctaatag gtaccgatcg ccactagcgc cgcggtcgga
     3481 atcagcgcac tggccgccgg agcggtcagc tacctgttgc tgcgccgctt cgctttctag
     3541 caccgacgtc ccggcgccag cagctacctc tcgagcaaac gttttgcgcg cccgaaaaat
     3601 aagtcgttgc agaactttca tgaattaggc cttgctgcgc ccaggctcca gtagtagaaa
     3661 tggagtcacg gcagcccggt gaagccaagg tcgaaccgga agagaaggtt cgtcctcccg
     3721 acccgggcac ccagcacggc ccccggaacc cacgcggagt catagccgcg ataatggcag
     3781 aagtgttgcg ggcctgcgta attgcgaaat tcagatggtg ccgacggccc tttgggtggg
     3841 gctgcagcca gaagggtcgc aaaagcgccg aggccaaccc acgcagccca caaatgcacg
     3901 cttggtaacc gaggtaccag gaggaaggca aatatgacta aaaaaatttc attcattatt
     3961 aacggccagg ttgaaatctt tcccgaaggt gatgatttag tgcaatccat taattttggt
     4021 gataatagtg tttacctgcc aatattgaat gactctcatg taaaaaacat tattgattgt
     4081 aatggaaata acgaattacg gttgcataac attgtcaatt ttctctatac ggtagggcaa
     4141 agatggaaaa atgaagaata ctcaagacgc aggacataca ttcgtgactt aaaaaaatat
     4201 atgggatatt cagaagaaat ggctaagcta gaggccaatt ggatatctat gattttatgt
     4261 tctaaaggcg gcctttatga tgttgtagaa aatgaacttg gttctcgcca tatcatggat
     4321 gaatggctac ctcaggatga aagttatgtt cgggcttttc cgaaaggtaa atctgtacat
     4381 ctgttggcag gtaatgttcc attatctggg atcatgtcta tattacgcgc aattttaact
     4441 aagaatcagt gtattataaa aacatcgtca accgatcctt ttaccgctaa tgcattagcg
     4501 ttaagtttta ttgatgtaga ccctaatcat ccgataacgc gctctttatc tgttatatat
     4561 tggccccacc aaggtgatac atcactcgca aaagaaatta tgcaacatgc ggatgttatt
     4621 gtcgcttggg gagggccaga tgcgattaat tgggcggtag agcatgcgcc atcttatgct
     4681 gatgtgatta aatttggttc taaaaagagt ctttgcatta tcgataatcc tgttgatttg
     4741 acgtccgcag cgacaggtgc ggctcatgat gtttgttttt acgatcagcg agcttgtttt
     4801 tctgcccaaa acatatatta catgggaaat cattatgagg aatttaagtt agcgttgata
     4861 gaaaaactta atctatatgc gcatatatta ccgaatgcca aaaaagattt tgatgaaaag
     4921 gcggcctatt ctttagttca aaaagaaagc ttgtttgctg gattaaaagt agaggtggat
     4981 attcatcaac gttggatgat tattgagtca aatgcaggtg tggaatttaa tcaaccactt
     5041 ggcagatgtg tgtaccttca tcacgtcgat aatattgagc aaatattgcc ttatgttcaa
     5101 aaaaataaga cgcaaaccat atctattttt ccttgggagt catcatttaa atatcgagat
     5161 gcgttagcat taaaaggtgc ggaaaggatt gtagaagcag gaatgaataa catatttcga
     5221 gttggtggat ctcatgacgg aatgagaccg ttgcaacgat tagtgacata tatttctcat
     5281 gaaaggccat ctaactatac ggctaaggat gttgcggttg aaatagaaca gactcgattc
     5341 ctggaagaag ataagttcct tgtatttgtc ccataatagg taaaaagtat ggaaaatgaa
     5401 tcaaaatata aaaccatcga ccacgttatt tgtgttgaag gaaataagaa aattcatgtt
     5461 tgggaaacgc tgccagaaga aaacagccca aagagaaaga atgccattat tattgcgtct
     5521 ggttttgccc gcaggatgga tcattttgct ggtctggcgg aatatttatc gcggaatgga
     5581 tttcatgtga tccgctatga ttcgcttcac cacgttggat tgagttcagg gacaattgat
     5641 gaatttacaa tgtctatagg aaagcagagc ttgttagcag tggttgattg gttaactaca
     5701 cgaaaaataa ataacttcgg tatgttggct tcaagcttat ctgcgcggat agcttatgca
     5761 agcctatctg aaatcaatgc ttcgttttta atcaccgcag tcggtgttgt taacttaaga
     5821 tattctcttg aaagagcttt agggtttgat tatctcagtc tacccattaa tgaattgccg
     5881 aataatctag attttgaagg ccataaattg ggtgctgaag tctttgcgag agattgtctt
     5941 gattttggtt gggaagattt agcttctaca attaataaca tgatgtatct tgatataccg
     6001 tttattgctt ttactgcaaa taacgataat tgggtcaagc aagatgaagt tatcacattg
     6061 ttatcaaata ttcgtagtaa tcgatgcaag atatattctt tgttaggaag ttcgcatgac
     6121 ttgagtgaaa atttagtggc cctgcgcaat ttttatcaat cggttacgaa agccgctatc
     6181 gcgatggata atgatcatct ggatattgat gttgatatta ctgaaccgtc atttgaacat
     6241 ttaactattg cgacagtcaa tgaacgccga atgagaattg agattgaaaa tcaagcaatt
     6301 tctctgtctt aaaggatcct gaggaggaaa acaggtatga cttcatatgt tgataaacaa
     6361 gaaattacag caagctcaga aattgatgat ttgatttttt cgagcgatcc attagtgtgg
     6421 tcttacgacg agcaggaaaa aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat
     6481 tataaacatt gtcgagaata tcgtcactac tgtcaggcac acaaagtaga tgacaatatt
     6541 acggaaattg atgacatacc tgtattccca acatcggttt ttaagtttac tcgcttatta
     6601 acttctcagg aaaacgagat tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa
     6661 agtcaggtgg cgcgtgacag attaagtatt gagagactct taggctctgt gagttatggc
     6721 atgaaatatg ttggtagttg gtttgatcat caaatagaat tagtcaattt gggaccagat
     6781 agatttaatg ctcataatat ttggtttaaa tatgttatga gtttggtgga attgttatat
     6841 cctacgacat ttaccgtaac agaagaacga atagattttg ttaaaacatt gaatagtctt
     6901 gaacgaataa aaaatcaagg gaaagatctt tgtcttattg gttcgccata ctttatttat
     6961 ttactctgcc attatatgaa agataaaaaa atctcatttt ctggagataa aagcctttat
     7021 atcataaccg gaggcggctg gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc
     7081 aatcatcttt tatttgatac tttcaatctc agtgatatta gtcagatccg agatatattt
     7141 aatcaagctg aactcaacac ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg
     7201 ccgtgggtat atgcgcgagc gcttgatcct gaaacgttga aacctgtacc tgatggaacg
     7261 ccggggttga tgagttatat ggatgcgtca gcaaccagtt atccagcatt tattgttacc
     7321 gatgatgtcg ggataattag cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt
     7381 ttacgtcgcg tcaatacgag gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt
     7441 gatagttgat atccatcaag cttatcgatg tcgacgtagt taactagcgt acgatcgact
     7501 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatgcca
     7561 tcatggccgc ggtgatcagc tagagccgtg aacgacaggg cgaacgccag cccgccgacg
     7621 gcgagggttc cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact
     7681 gcaccagctc caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat
     7741 tcgtttgtca gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg
     7801 tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa
     7861 caccctctga ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa
     7921 tgggtgtctg ccgaccacat atatgggccg gtcaagatag gtttttaccc cctctcggct
     7981 gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc
     8041 acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc
     8101 ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg
     8161 caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc
     8221 tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg
     8281 gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg
     8341 gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc
     8401 tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg
     8461 tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg
     8521 gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag
     8581 cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc
     8641 gccgctgaga tcttcgtgca ctaccggatc gcggcataca tcctggcgtg gacgagcctc
     8701 cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg
     8761 aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc
     8821 aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga
     8881 gcgcacatga aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg
     8941 acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc
     9001 aagatcggtc ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc
     9061 gctcaggcag gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg
     9121 atggcgatga agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg
     9181 tccaagctgg ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc
     9241 taaagagggg ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac
     9301 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
     9361 cgccagctag ccacctgacg tcgggggggg gggaaagcca cgttgtgtct caaaatctct
     9421 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     9481 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
     9541 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     9601 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt
     9661 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     9721 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     9781 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat
     9841 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     9901 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     9961 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
    10021 ggcctgttga acaagtctgg aaagaaatgc ataatctttt gccattctca ccggattcag
    10081 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
    10141 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
    10201 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
    10261 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
    10321 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
    10381 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
    10441 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
    10501 caaagctctc accaaccgtg gctccctcac tttctggctg g
//
