LOCUS       sequence-258647-        7019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-UBF1 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Newhart A, Powers SL, Shastrula PK, Sierra I, Joo LM, Hayden JE, 
            Cohen AR, Janicki SM
  TITLE     RNase P protein subunit Rpp29 represses histone H3.3 nucleosome 
            deposition.
  JOURNAL   Mol Biol Cell. 2016 Apr 1;27(7):1154-69. doi: 
            10.1091/mbc.E15-02-0099. Epub 2016 Feb 3.
  PUBMED    26842893
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-02-0099"; journalName: "Mol Biol Cell"; date: 
            "2016-04-1- 1"; volume: "27"; issue: "7"; pages: "1154-69"
COMMENT     Sequence Label: YFP-UBF1-C1
FEATURES             Location/Qualifiers
     source          1..7019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3430..3441
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    3807..3928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3844..3863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3898..3917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3935..4390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4072..4093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4284..4303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4417..4521
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4523..4880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4523..4714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4546..4566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4731..4866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4915..5709
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4915..5709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4969..4988)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5579..5598
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5897..5916)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5941..5988
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6317..6905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6806..6825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atgaacggag aagccgactg ccccacagac ctggaaatgg ccgcccccaa aggccaagac
     1441 cgttggtccc aggaagacat gctgactttg ctggaatgca tgaagaacaa ccttccatcc
     1501 aatgacagct ccaagttcaa aaccaccgaa tcacacatgg actgggaaaa agtagcattt
     1561 aaagactttt ctggagacat gtgcaagctc aaatgggtgg agatttctaa tgaggtgagg
     1621 aagttccgta cattgacaga attgatcctc gatgctcagg aacatgttaa aaatccttac
     1681 aaaggcaaaa aactcaagaa acacccagac ttcccaaaga agcccctgac cccttatttc
     1741 cgcttcttca tggagaagcg ggccaagtat gcgaaactcc accctgagat gagcaacctg
     1801 gacctaacca agattctgtc caagaaatac aaggagcttc cggagaagaa gaagatgaaa
     1861 tatattcagg acttccagag agagaaacag gagttcgagc gaaacctggc ccgattcagg
     1921 gaggatcacc ccgacctaat ccagaatgcc aagaaatcgg acatcccaga gaagcccaaa
     1981 accccccagc agctgtggta cacccacgag aagaaggtgt atctcaaagt gcggccagat
     2041 gccactacga aggaggtgaa ggactccctg gggaagcagt ggtctcagct ctcggacaaa
     2101 aagaggctga aatggattca taaggccctg gagcagcgga aggagtacga ggagatcatg
     2161 agagactata tccagaagca cccagagctg aacatcagtg aggagggtat caccaagtcc
     2221 accctcacca aggccgaacg ccagctcaag gacaagtttg acgggcgacc caccaagcca
     2281 cctccgaaca gctactcgct gtactgcgca gagctcatgg ccaacatgaa ggacgtgccc
     2341 agcacagagc gcatggtgct gtgcagccag cagtggaagc tgctgtccca gaaggagaag
     2401 gacgcctatc acaagaagtg tgatcagaaa aagaaagatt acgaggtgga gctgctccgt
     2461 ttcctcgaga gcctgcctga ggaggagcag cagcgggtct tgggggaaga gaagatgctg
     2521 aacatcaaca agaagcaggc caccagcccc gcctccaaga agccagccca ggaagggggc
     2581 aagggcggct ccgagaagcc caagcggccc gtgtcggcca tgttcatctt ctcggaggag
     2641 aaacggcggc agctgcagga ggagcggcct gagctctccg agagcgagct gacccgcctg
     2701 ctggcccgaa tgtggaacga cctgtctgag aagaagaagg ccaagtacaa ggcccgagag
     2761 gcggcgctca aggctcagtc ggagaggaag cccggcgggg agcgcgagga acggggcaag
     2821 ctgcccgagt cccccaaaag agctgaggag atctggcaac agagcgttat cggcgactac
     2881 ctggcccgct tcaagaatga ccgggtgaag gccttgaaag ccatggaaat gacctggaat
     2941 aacatggaaa agaaggagaa actgatgtgg attaagaagg cagccgaaga ccaaaagcga
     3001 tatgagagag agctgagtga gatgcgggca cctccagctg ctacaaattc ttccaagaag
     3061 atgaaattcc agggagaacc caagaagcct cccatgaacg gttaccagaa gttctcccag
     3121 gagctgctgt ccaatgggga gctgaaccac ctgccgctga aggagcgcat ggtggagatc
     3181 ggcagtcgct ggcagcgcat ctcccagagc cagaaggagc actacaaaaa gctggccgag
     3241 gagcagcaaa agcagtacaa ggtgcacctg gacctctggg ttaagagcct gtctccccag
     3301 gaccgtgcag catataaaga gtacatctcc aataaacgta agagcatgac caagctgcga
     3361 ggcccaaacc ccaaatccag ccggactact ctgcagtcca agtcggagtc cgaggaggat
     3421 gatgaagagg atgaggatga cgaggacgag gatgaagaag aggaagatga tgagaatggg
     3481 gactcctctg aagatggcgg cgactcctct gagtccagca gcgaggacga gagcgaggat
     3541 ggggatgaga atgaagagga tgacgaggac gaagacgacg acgaggatga cgatgaggat
     3601 gaagataatg agtccgaggg cagcagctcc agctcctcct cctcagggga ctcctcagac
     3661 tctgactcca actgacggga tccaccggat ctagataact gatcataatc agccatacca
     3721 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3781 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3901 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3961 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4021 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4081 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4141 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4201 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4261 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4321 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4381 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4501 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4561 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4621 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4681 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4741 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4801 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4861 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4921 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4981 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5041 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     5101 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5161 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5221 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5281 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5341 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5401 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     5461 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5521 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5581 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5641 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5701 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5761 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5821 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5881 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5941 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     6001 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     6061 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     6121 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     6181 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6241 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
