LOCUS       sequence-25865-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Haudenshield JS, Hartman GL
  TITLE     Exogenous Controls Increase Negative Call Veracity in Multiplexed, 
            Quantitative PCR Assays for Phakopsora pachyrhizi.
  JOURNAL   Plant Dis. 2011 Mar;95(3):343-352. doi: 10.1094/PDIS-01-10-0023.
  PUBMED    30743502
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Dis"; date: "2011-03"; volume: "95"; issue: "3"; pages: 
            "343-352"
COMMENT     Sequence Label: pJSH-B14
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..30
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12..30
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     59..75
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     60..76
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             217..228
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(257..273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(257..273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(302..321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(303..321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(331..348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(331..347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(340..362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgccagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc
       61 gaggtcgacg gtatcgataa gcttgatatc cactgtggaa ttcgcccttc acgcctagga
      121 cgagaactcc cacatcgagc ttgacgcaaa cgaccacgcc aggaccatgg caaacatcaa
      181 caccgagcgc aacgccttgt gctgaaacac ccgctgattg aagggcgaat tccacattgg
      241 tcgctgcagc ccgggggatc cactagttct agagcggccg caccgcggga gctccaattc
      301 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
      361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      421 gcgtaatagc gaagaggccc gcaccgatta aattttggtc atgagattat caaaaaggat
      481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      721 agatttatca gcaataaacc agcc
//
