LOCUS       sequence-258663-       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of human MCOLN1 in mammalian cells, GFP-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Kasitinon SY, Eskiocak U, Martin M, Bezwada D, Khivansara V, 
            Tasdogan A, Zhao Z, Mathews T, Aurora AB, Morrison SJ
  TITLE     TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively 
            Regulating MAPK and mTORC1 Signaling.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2293-2305.e9. doi: 
            10.1016/j.celrep.2019.07.086.
  PUBMED    31461647
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2293-2305.e9"
COMMENT     Sequence Label: pLOC-MCOLN1
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4598..5170
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4765..4782)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4992..5011
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5176..5196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5197..5889
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="ESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMK
                     STKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLH
                     VSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRT
                     FSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFK
                     TPDADAGEE"
     misc_feature    6439..7027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6492..6512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6910..6921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7234..7467
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7568..7585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7574..7798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7844..8272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7931..7950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8141..8162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8281..8301)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8286..8615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8466..8601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8528..8547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(8752..9340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8832..8851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9511..10302,10303..10371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10134..10153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10372..10476)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10524..10658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10561..10580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10615..10634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagatta attaagttta aacttaagct tggtaccgag
     2821 ctcggatcca tgacagcccc ggcgggtccg cgcggctcag agaccgagcg gcttctgacc
     2881 cccaaccccg ggtatgggac ccaggcgggg ccttcaccgg cccctccgac acccccagaa
     2941 gaggaagacc ttcgccgtcg tctcaaatac tttttcatga gtccctgcga caagtttcga
     3001 gccaagggcc gcaagccctg caagctgatg ctgcaagtgg tcaagatcct ggtggtcacg
     3061 gtgcagctca tcctgtttgg gctcagtaat cagctggctg tgacattccg ggaagagaac
     3121 accatcgcct tccgacacct cttcctgctg ggctactcgg acggagcgga tgacaccttc
     3181 gcagcctaca cgcgggagca gctgtaccag gccatcttcc atgctgtgga ccagtacctg
     3241 gcgttgcctg acgtgtcact gggccggtat gcgtatgtcc gtggtggggg tgacccttgg
     3301 accaatggct cagggcttgc tctctgccag cggtactacc accgaggcca cgtggacccg
     3361 gccaacgaca catttgacat tgatccgatg gtggttactg actgcatcca ggtggatccc
     3421 cccgagcggc cccctccgcc ccccagcgac gatctcaccc tcttggaaag cagctccagt
     3481 tacaagaacc tcacgctcaa attccacaag ctggtcaatg tcaccatcca cttccggctg
     3541 aagaccatta acctccagag cctcatcaat aatgagatcc cggactgcta taccttcagc
     3601 gtcctgatca cgtttgacaa caaagcacac agtggacgga tccccatcag cctggagacc
     3661 caggcccaca tccaggagtg taagcacccc agtgtcttcc agcacggaga caacagcttc
     3721 cggctcctgt ttgacgtggt ggtcatcctc acctgctccc tgtccttcct cctctgcgcc
     3781 cgctcactcc ttcgaggctt cctgctgcag aacgagtttg tggggttcat gtggcggcag
     3841 cggggacggg tcatcagcct gtgggagcgg ctggaatttg tcaatggctg gtacatcctg
     3901 ctcgtcacca gcgatgtgct caccatctcg ggcaccatca tgaagatcgg catcgaggcc
     3961 aagaacttgg cgagctacga cgtctgcagc atcctcctgg gcacctcgac gctgctggtg
     4021 tgggtgggcg tgatccgcta cctgaccttc ttccacaact acaatatcct catcgccaca
     4081 ctgcgggtgg ccctgcccag cgtcatgcgc ttctgctgct gcgtggctgt catctacctg
     4141 ggctactgct tctgtggctg gatcgtgctg gggccctatc atgtgaagtt ccgctcactc
     4201 tccatggtgt ctgagtgcct gttctcgctc atcaatgggg acgacatgtt tgtgacgttc
     4261 gccgccatgc aggcgcagca gggccgcagc agcctggtgt ggctcttctc ccagctctac
     4321 ctttactcct tcatcagcct cttcatctac atggtgctca gcctcttcat cgcgctcatc
     4381 accggcgcct acgacaccat caagcatccc ggcggcgcag gcgcagagga gagcgagctg
     4441 caggcctaca tcgcacagtg ccaggacagc cccacctccg gcaagttccg ccgcgggagc
     4501 ggctcggcct gcagccttct ctgctgctgc ggaagggacc cctcggagga gcattcgctg
     4561 ctggtgaatt gagctagcta atgaaccggg cgcgccgccc ctctccctcc ccccccccta
     4621 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     4681 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     4741 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     4801 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     4861 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     4921 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     4981 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5041 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5101 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5161 cacgataata ccatgcccaa gaagaagaga aaggtggaga gcgacgagag cggcctgccc
     5221 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5281 ggcggcggag agggcacccc cgagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5341 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     5401 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     5461 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     5521 taccgctacg aggccggccg cgtgatcggc gacttcaagg tgatgggcac cggcttcccc
     5581 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5641 caccccatgg gcgataacga tctggatggc agcttcaccc gcaccttcag cctgcgcgac
     5701 ggcggctact acagctccgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5761 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga ggatcacagc
     5821 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agaccccgga tgcagatgcc
     5881 ggtgaagaaa gatctcgagc tcaagcttcg aattctgcag tcgagcagct gttgaatttt
     5941 gaccttctta agcttgcggg agacgtcgag tccaaccctg ggcccggtac caggagggcc
     6001 accatgaaga ccttcaacat ctctcagcag gatctggagc tggtggaggt cgccactgag
     6061 aagatcacca tgctctatga ggacaacaag caccatgtcg gggcggccat caggaccaag
     6121 actggggaga tcatctctgc tgtccacatt gaggcctaca ttggcagggt cactgtctgt
     6181 gctgaagcca ttgccattgg gtctgctgtg agcaacgggc agaaggactt tgacaccatt
     6241 gtggctgtca ggcaccccta ctctgatgag gtggacagat ccatcagggt ggtcagcccc
     6301 tgtggcatgt gcagagagct gatctctgac tatgctcctg actgctttgt gctcattgag
     6361 atgaatggca agctggtcaa aaccaccatt gaggaactca tccccctcaa gtacaccagg
     6421 aactaataat aagttaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6841 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7021 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     7081 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7141 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7201 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     7261 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7321 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7381 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7441 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7501 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgt ttaaacccgc
     7561 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7681 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7741 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7801 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     7861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     8281 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8341 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8461 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8521 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     8581 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     8641 ttttcggatc tgatcagcac gtgatgaaaa agcctgaact caccgcgacg tcatgtgagc
     8701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10501 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    10561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10621 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10681 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10741 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10801 aaatatgtac tacaaactta gtagt
//
