LOCUS       sequence-258677-        5816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination plasmid containing the 35S:XVE estradiol inducible 
            driver and the A-G overhangs for GreenGate cloning of an expression 
            casette for estradiole-inducible expression in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Denay G, Schultz P, Hansch S, Weidtkamp-Peters S, Simon R
  TITLE     Over the rainbow: A practical guide for fluorescent protein 
            selection in plant FRET experiments.
  JOURNAL   Plant Direct. 2019 Dec 6;3(12):e00189. doi: 10.1002/pld3.189. 
            eCollection 2019 Dec.
  PUBMED    31844834
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2019-12-6"; volume: "3(12):e00189. doi"; pages: " 
            10.1002/pld3.189. eCollection 2019 Dec"
COMMENT     Sequence Label: pGD283
FEATURES             Location/Qualifiers
     source          1..5816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        452..797
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     740..760
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    825..880
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             886..1137
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             1153..1386
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             1396..2331
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1444..2334
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      2391..3032
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    3138..3162
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3253..3841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3333..3352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4012..4800)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5091..5526
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5653..5675
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 cacagatggt tagagaggct tacgcagcac gtctcatcaa gacgatctac ccgagcaata
       61 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      121 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      181 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      241 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      301 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      361 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      421 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      481 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      541 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      601 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      661 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
      721 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
      781 ttcatttgga gagaacacgg gggacgagct aacatatttt tacaacaatt accaacaaca
      841 acaaacaaca aacaacatta caattactat ttacaattac aggctatgaa agcgttaacg
      901 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
      961 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
     1021 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
     1081 attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
     1141 gaaccgtcga gcgccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc
     1201 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     1261 gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg
     1321 gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac
     1381 ggtggggatc cgtctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
     1441 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
     1501 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
     1561 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
     1621 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
     1681 cttctagaat gtgcctggct agagatcctg atgattggac tcgtctggcg ctccatggag
     1741 cacccaggga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
     1801 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
     1861 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
     1921 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
     1981 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
     2041 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
     2101 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
     2161 gacctgctgc tggagatgct ggacgcccac cgcctacatg cgcccactag ccgtggaggg
     2221 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
     2281 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agtctcaggt
     2341 ggatcctaga taacctttac cttcattccc tgtaaattgg taccctgcaa agctttcgtt
     2401 cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca
     2461 ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     2521 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     2581 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     2641 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     2701 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     2761 gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca
     2821 gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt
     2881 tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat
     2941 aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta
     3001 tgacttgcca attgattgac aacatgcatc aatcgactat gagtaggcaa gatgttctgt
     3061 aggaccttga gaccgaaaag gtggtctcag tattctagac caagcatgca cagctggcgt
     3121 tctcagccag atccccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt
     3181 tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3721 gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3961 atgaagtttt aaatcaatct aaagtatata tgtgtaacat tggtctagtg attatttgcc
     4021 gactaccttg gtgatctcgc ctttcacgta gtgaacaaat tcttccaact gatctgcgcg
     4081 cgaggccaag cgatcttctt gtccaagata agcctgccta gcttcaagta tgacgggctg
     4141 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     4201 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     4261 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     4321 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     4381 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     4441 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     4501 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     4561 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     4621 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     4681 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     4741 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     4801 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     4861 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
     4921 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
     4981 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa
     5041 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc ccatccaaca
     5101 gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg tagttatcca
     5161 cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc ccgctccaaa
     5221 aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga gtacgtgaaa
     5281 tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg ctaatagccc
     5341 tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag caagtagtat
     5401 gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc ttggcttgtg
     5461 gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt gcccaccgtc
     5521 gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata aatttttttt
     5581 tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt tccgatcccc
     5641 ggaattagat cttggcagga tatattgtgg tgtaacgttc tcgaactagg ctcggacgaa
     5701 gtaagcggcc gcaaccttaa ttaaacacgg cgcgccaaca gcgatcgcac caggtaccac
     5761 ctccgatcca ttcggtcccc agattagcct tttcaatttc agaaagaatg ctaacc
//
