LOCUS       sequence-258694-        9526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of 
            INS-1::GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH1922
FEATURES             Location/Qualifiers
     source          1..9526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..32
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    149..170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(185..190,191..208,209..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    223..239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     247..263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      5030..5039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5036..5038,5039..5041,5042..5755)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5036..5038,5039..5041,5042..5752)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5081..5102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5342..5361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5689..5710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5780..5804)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5830..5871
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6697..6714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6697..6713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6706..6728)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6926..7381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7013..7032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7223..7244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7398..7417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7517..7539
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7577..7595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7663..7767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7768..7836,7837..8628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7986..8005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8799..9387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9288..9307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
       61 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      241 ttcacacagg aaacagctat gacatgatta cgaattcgaa tggccatggg acgtcgacgc
      301 ttgcatgcct gcagcgtttc cgataccccc ttatatcagc acacattcag tcatcatacc
      361 acaaaatgtt gtgatagtga taggcaaaac taccgtattt cctctattaa tcttgcatgc
      421 aagactaatt ttcgattaac ccgtaggggt gcaagactaa tagagactgc aagactatta
      481 gaggctgaaa tactaatttt cgtatgctca ataattttgg aaattggcct attttttgta
      541 gaaacttgat accgtttaaa caaggaaaaa tacacacttt ttaatatttc atcaataatt
      601 tgaacgattt tgtgatttta agttcaattt gcccaaaaaa agacaatttt tctgacgtta
      661 ccgcaaagta atgctggtca ggcaaaaaga gcggtgcaaa aatataagag actgcaatac
      721 taatagagga aatacggtaa ttgaatttta gtgaaacttg cgacagtttt cctcattttt
      781 ttgttattcc ggcatacgag tgtggcatac gagtgaggtc atcttttgtt tcttccgttt
      841 cttcatcgct tttgaaaaaa aatgttgaag aaaacttcgt agccgatagc cggataatgt
      901 ttggcacggc gttccaacat atatcattgg aaatttttta agatttttgc gaaaaatcac
      961 attcttcacg atgagaacac gttattgaag gaatataaat caagaataac atatagttat
     1021 atttctctat tactttaacg ttaaatatga gcaaatttga gcattttgat tgcgatgaaa
     1081 agcagaatcg agtcaactga aatccgttca aaaacctaag ctcggttcgc ccgaaagtcg
     1141 atttttccaa tagccgaaca gcactgcttc tttattttcc aggtattttt ggggtgtaag
     1201 aattatatct tctttgtttg ggtatttacg tttccgaatt ccctttcttg atctgtcgct
     1261 atttttcaga aaccagaaca gtttatcctt tttcatgaga acctgaatag ctcaaaaacc
     1321 cgtttaattt cttttcatca tctcatttga aactttccta gtcttcttaa tgatttccag
     1381 gctcctccta catttgtatc tcaagatgca tacactttat ttccgctcca attcgaatca
     1441 tctacttctc ttttttcttc aactttctac ttttttccat tcttttcttt caaatgttcg
     1501 acgtcttcaa gtgactgctc ttcattctcc tcatttttct cgaatttcat tcttgtctct
     1561 tttttgctca aaaatggaaa atgaaagtga cgtgagattt ggacggcggg acacgggggc
     1621 agtagaagca gcaaaaagga gagaaagagg acacaaataa gaagaacgaa ttcaaaaata
     1681 agcggagagg agctatttcc gtcaattcta cctccccaat cttcatcaat tcggctcaat
     1741 tgaatgacgt cacagggaga taaaccgttt ggatgagcgc cgtccatcac tgacgccatc
     1801 ccgtttggga caagaaaaga gaaaaaagag cacaaagttt ttggtgacgg atcttgtcaa
     1861 tcatatgaaa gttgttctga ttgattgtca gtttttttcc tacttttttg gattctatcc
     1921 acttctgaac ttttgacaag tttcaaactt tctgaatcat tttctatgca ttttcctgga
     1981 attcttttta tgtaaaatat gaaatagaat gtttttgaat tcaagttctg cttttttctt
     2041 cttttctgtt ctgttcgggc ttgggtatgc tttttttaaa aattattttg cacatcgacc
     2101 aataagtgcg caacttataa aattaattta tttttgttaa tttttgaaat acttgtattg
     2161 ctttaagtga tctgacctcg ccctgagctt tccacgtagt tatcaaatac aaatcctcca
     2221 agggtaacgt acctatatta ctgatcttta taataacttt atcacctgtc cagttccaga
     2281 ggaattctgt taagcttata ggcacagaag gagtcatttc tgctggtttt taatgatcca
     2341 aatctttatt tcaagtaaaa aactgaacac ttgcgaataa aactatcaga ttaaccattc
     2401 accaaaaatg tgtttgaatc taaaactttc tcagtattcc aaatatagaa ataaataacc
     2461 acgacattgc taaaatctgt ctgaattgtg tactccttac cgtgaagtaa taatggatat
     2521 aatgaatcgt ttgaaatgaa tgatcagcac atttttggtg aaagatcaca aataaggaat
     2581 aagcgtcgga aaataaaacg attatttcgg atcaaaaatt tgttgaagat catatacact
     2641 cgagaccaag attattctag acaattttca aattggcttc tttgtttgca aatcttcata
     2701 ataatctgtg aagtttggaa atttgaattt ttaatctttt ttcatagatt atagttttta
     2761 tttctttgca aaactatatt aaaaaccgat gcattgtttt agggaaatta atgagccttt
     2821 tgttcaacac taaaaacaat aaaattaaaa ttttggcttc atcatttgac cttttttaag
     2881 ttcgaaaact tttctcgtat ttctgaaccg ccaatttttt cacacatctc tagacttttg
     2941 gtgcccgttc cagaaagtta agtaattgct attctaagaa agttctcaac attgtttttt
     3001 agttctgatt gaattctgat gttccaggaa tatattttaa atttaatatt ctttgcacta
     3061 tttctatact aactaaataa taaatagtct aagtatgttc aatgagaaca aaaaagctct
     3121 atctatattt ttctatccta tttcatttat atccttttca ttttgaactc accagcttct
     3181 cttcttcttc ttgtccatat acttcttaaa agtctcgaac ttctctctct cactctccat
     3241 caatttcttt atggctaccg cttgcgctct gccgcttccg aagaaagaag gcgttggcag
     3301 agctctcaat tctatttttt ctcgccgtag gctatcgatt tctcagcttc tcttctctct
     3361 tcctcactcc accaccacca cttcgggctt cttcttttct tcattttcgt cttcttcttc
     3421 atcctcttct ttttttttca gttccccctc actcctcccc ttatatgcgc gtgcgagagg
     3481 ggtgcaaaag cagcgcgcat ccgagaattg aaacgagaaa acggagacgc agcagcagtt
     3541 cgtcctcaga aaaatagcca gtaaaaagag aaaaagatag agagagacct atcgcatttt
     3601 attttcaaat tgcaattcct gcaattcatg tgtgcgtgtg cccattttca attcttcccg
     3661 ttatttttca gaccattacc aacgttcttt ctagatcttg agacaatctt ccttctgctc
     3721 aatcgttcgt cgtgaagacg gcatcgacga cgacgacgat cagcagtaaa ggatcccatg
     3781 tactggtttc gtcaagttta caggtgagct tactttgaat cttgacgtcg aaaaagtcca
     3841 gacattattc atcttttacc ctgcatctta attcgaataa tttttccaga ccctcgttct
     3901 tctttggctt tctcgcgatc cttctcctct cgtcgccgac gccttcagac gcatcgattc
     3961 gactatgcgg atcacgtctc acaacaaccc ttttagcagt atgccggaat cagctgtgca
     4021 ctggattaac cgctttcaaa cgttagtaat tctcgatggg gtctccgggg gtggaaaacc
     4081 ttatgaaaca ttcacgatgc tcaaaaaagc tttttcttca aagataccaa tgttgaagtc
     4141 catattaatg cacttttgag atacctactg tttgatagat tttgagtttt gattgatggt
     4201 aattaactac gtcaagcttg tcatttgata gttgtctaga aaaagagaga taattataac
     4261 cacaagacaa ttttaatgtt agatttttta tattgtaagt catgagtgtt gagaatacgt
     4321 agacaaaaaa ccaaatggat gttgtcactt ttaaagtttg aaataggttc tgaagttgaa
     4381 aatcattaaa tgacgtataa ttataattca ctaatttagt cactgaatta gcttgaaatc
     4441 gaataaagtg catatatatt ccaaaataga agcaagagac aaaaataaat caagtattta
     4501 taagttaggg acaatgaata agtttttgaa atgattatga aactattaaa aacactgata
     4561 gactatcaat tacttgattt ttgtgagaaa aaaaaaaaca aatttgatac ggattgaaat
     4621 gatcagacaa taaaaaaacc gaaaccacac atgactcgtt cagcagaagt atggattgag
     4681 ctccacacag ccacacctca tattttgaat agatcacaac tcagatccat attacaaaaa
     4741 atatacaaac gcctgtaggc ctgtaggcac aagtaaacct ctgtgtgttc aatcaatata
     4801 atatattttg aaactcctca gtatttattc tccaatcaaa actacaatca aaatgtatta
     4861 ttcaggttcc gccgaccaat cctatgcacc aacaactcgc gatctttttc acattcacca
     4921 ccaacaaaag cgaggcggaa ttgcgacaga atgttgtgag aagcgatgtt catttgcata
     4981 tctcaaaaca ttctgctgca atcaggacga taatggggat ccaccggtcg ccaccatggt
     5041 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5101 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5161 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5221 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5281 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5341 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5401 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5461 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5521 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5581 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5641 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5701 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     5761 ccgcactcga gatatctaga cccagctttc ttgtacaaag tggttgatct agagggcccg
     5821 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     5881 tagtaatgag tttatctgag ctccgcatcg gccgctgtca tcagatcgcc atctcgcgcc
     5941 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     6001 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     6061 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     6121 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     6181 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     6241 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     6301 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     6361 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     6421 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     6481 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     6541 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     6601 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcta
     6661 ctagtggcca cgtgggccgt gcaccttaag cttggcactg gccgtcgttt tacaacgtcg
     6721 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6781 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6841 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6901 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6961 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7021 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7081 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7141 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7201 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7261 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7321 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     7381 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7441 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7501 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7561 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7621 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7681 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7981 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8281 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagcc
//
